SPECTROPHOTOMETRY OF FAIRLEY’S NEW BLOOD PIGMENT, METHEMALBUMIN. Fox CL.J Clin Invest. 1941 Sep;20(5):603-6. doi: 10.1172/JCI101253.PMID: 16694865 Free PMC article. No abstract available.

Blackwater Fever and Methæmalbumin. Lowe J.Ind Med Gaz. 1944 Oct;79(10):483-484.PMID: 29014105 Free PMC article. No abstract available.

METHEMALBUMIN. I. APPEARANCE DURING ADMINISTRATION OF PAMAQUINE AND QUININE. Rosenfeld M, Zubrod CG, Blake WD, Shannon JA.J Clin Invest. 1948 May;27(3 Pt 2):138-43. doi: 10.1172/JCI101953.PMID: 16695627 Free PMC article. No abstract available. 

METHEMALBUMIN. II. EFFECT OF PAMAQUINE AND QUININE ON PATHWAYS OF HEMOGLOBIN METABOLISM. Blake WD.J Clin Invest. 1948 May;27(3 Pt 2):144-50. doi: 10.1172/JCI101954.PMID: 16695628 Free PMC article. No abstract available. 

Methemalbumin; appearance during administration of pamaquine and quinine. ROSENFELD M, ZUBROD CG, et al.J Clin Invest. 1948 May;27(3 Pt1):138-43.PMID: 18860193 No abstract available.

Methemalbumin; effect of pamaquine on pathways of hemoglobin metabolism. BLAKE WD.J Clin Invest. 1948 May;27(3 Pt1):144-50.PMID: 18860194 No abstract available.

[Quantitative determination of methemoglobin and of methemalbumin in the blood]. FLEISCH H.Helv Physiol Pharmacol Acta. 1959;17:318-28.PMID: 13823405 French. No abstract available.

[The measure of hemolysis by the determination in the plasma of hemoglobin, methemalbumin and their derivatives]. FLEISCH H.Helv Med Acta. 1960 Jul;27:383-407.PMID: 13700177 French. No abstract available.

[Haptoglobin-methemoglobin bonds and formation of methemalbumin in the blood of the newborn infant and of normal children]. VECCHIO F, MIRAGLIA DEL GIUDICE M, RIGILLO N.Boll Soc Ital Biol Sper. 1960 Nov 30;36:1245-9.PMID: 13780527 Italian. No abstract available.

[Haptoglobulin-hemoglobin bonds and formation of methemalbumin in the blood of normal children]. VECCHIO F, MIRAGLIA DEL GIUDICE M, RIGILLO N.Boll Soc Ital Biol Sper. 1960 Nov 30;36:1239-42.PMID: 13780528 Italian. No abstract available.

[Haptoglobin-hemoglobin bonds and formation of methemalbumin in the blood of the newborn]. VECCHIO F, RIGILLO N, MIRAGLIA DEL GIUDICE M.Boll Soc Ital Biol Sper. 1960 Nov 30;36:1243-5.PMID: 13780530 Italian. No abstract available.

Haemin-protein binding in peroxidase and methaemalbumin. MAEHLY AC.Nature. 1961 Nov 18;192:630-2. doi: 10.1038/192630a0.PMID: 14468162 No abstract available.

Methaemalbumin in the differential diagnosis of acute haemorrhagic and oedematous pancreatitis. NORTHAM BE, ROWE DS, WINSTONE NE.Lancet. 1963 Feb 16;1(7277):348-52. doi: 10.1016/s0140-6736(63)91380-1.PMID: 13938985 No abstract available.

Methaemalbumin in the diagnosis of pancreatitis. RICHARDSON RW, GLICK S, BATES A, SHINTON NK.Lancet. 1963 Mar 16;1(7281):608-9. doi: 10.1016/s0140-6736(63)92724-7.PMID: 13982128 No abstract available.

[HEMOGLOBIN, METHEMALBUMIN AND HAPTOGLOBINEMIA IN PLASMA OF NEWBORNS WITH HEMOLYTIC JAUNDICE DUE TO ERYTHROCYTE GLUCOSE-6-PHOSPHATE DEHYDROGENASE DEFICIENCY. ELECTROPHORETIC RESEARCH]. RIGILLO N.Pediatria (Napoli). 1964 Jan-Feb;72:64-70.PMID: 14160781 Italian. No abstract available.

[STATISTICAL EVALUATION OF THE SERUM HAPTOGLOBIN AND METHEMALBUMIN TITERS IN NORMAL SUBJECTS AND IN CONSTITUTIONALLY HYPERHEMOLYTIC SUBJECTS]. CONTI C, MUZZOLINI M.Rass Fisiopatol Clin Ter. 1964 Mar-Apr;36:117-42.PMID: 14161800 Italian. No abstract available.

[STATISTICAL FINDINGS ON THE SERUM LEVEL OF HAPTOGLOBIN AND METHEMALBUMIN IN NORMAL SUBJECTS]. CENTURELLI G, NUZZI R, DALFABBRO G, MUZZOLINI M.Prog Med (Napoli). 1964 Mar 15;20:129-35.PMID: 14214822 Italian. No abstract available.

[STUDIES ON THE HAPTOGLOBIN AND METHEMALBUMIN IN THE BLOOD, BLOOD TRACES AND SPERMATOZOA WITH STARCH GEL ELECTROPHORESIS]. HEIFER U.Blut. 1964 Jun;10:113-9. doi: 10.1007/BF01630795.PMID: 14250503 German. No abstract available.

THE EFFECT OF CHONDROMUCOPROTEIN ON THE SOLUBILITY AND PEROXIDATIC PROPERTIES OF HAEMOGLOBIN AND METHAEMALBUMIN IN AQUEOUS SOLUTION AND BLOOD. ANDERSON AJ.Biochem J. 1965 Feb;94(2):401-9. doi: 10.1042/bj0940401.PMID: 14348200 Free PMC article.

Insoluble complexes, formed by electrostatic interaction between chondromucoprotein and chromoproteins (haemoglobin, methaemalbumin), were studied by measurement of precipitated pigment and by decrease in peroxidatic activity, maximum formation from aqueous solution occurr …

• Chondromucuprotein is a very interesting word that as far as I can tell has been replaced by others similar (or not). Rarely a good deed. I don’t have much to add on that at this time. I’m going to include wikipedia notes on “mucoprotein” and at least one article before I wrap up this list.
  • A mucoprotein is a glycoprotein composed primarily of mucopolysaccharides. Mucoproteins can be found throughout the body, including the gastrointestinal tract, reproductive organs, airways, and the synovial fluid of the knees. They are called mucoproteins because the carbohydrate quantity is more than 4% unlike glycoproteins where the carbohydrate quantity is less than 4%. Mucoprotein is produced in the cecum of the gastrointestinal tract. During gallbladder cancer, mucoprotein is over expressed. Sustaining a brain injury will lead to decreased mucoprotein production. The result is an alteration of gut microbiota as seen in mice.
  • Function – Mucoproteins are the proteins that are the building blocks of mucus, which is a protective barrier to the epithelia of cells. It is semipermeable, so it acts as a barrier to most bacteria and pathogens, while allowing for the uptake of nutrients, water, and hormones.
    • Cone, Richard A. (2009-02-27). “Barrier properties of mucus”. Advanced Drug Delivery Reviews. 61 (2): 75–85. doi:10.1016/j.addr.2008.09.008. ISSN 0169-409X. PMID 19135107.
  • Protein Structure – Mucoproteins are composed of o-linked carbohydrates as well as highly glycosylated proteins, which are held together by disulfide bonds. The viscosity of the mucus depends on the strength of the disulfide bonds. When these disulfide bonds are broken, the viscosity of the mucus secretions is reduced.
    • Aksoy, Murat; Guven, Suleyman; Tosun, Ilknur; Aydın, Faruk; Kart, Cavit (2012-09-01). “The effect of ethinyl estradiol and drospirenone-containing oral contraceptives upon mucoprotein content of cervical mucus”. European Journal of Obstetrics & Gynecology and Reproductive Biology. 164 (1): 40–43. doi:10.1016/j.ejogrb.2012.05.002. ISSN 0301-2115. PMID 22633169.
    • Houlden, A.; Goldrick, M.; Brough, D.; Vizi, E.S.; Lénárt, N.; Martinecz, B.; Roberts, I.S.; Denes, A. (October 2016). “Brain injury induces specific changes in the caecal microbiota of mice via altered autonomic activity and mucoprotein production”. Brain, Behavior, and Immunity. 57: 10–20. doi:10.1016/j.bbi.2016.04.003. PMC 5021180. PMID 27060191.
    • Kumar, Puneet; Shukla, Priyesh; Kumari, Soni; Dixit, Ruhi; Narayan, Gopeshwar; Dixit, V. K.; Khanna, A. K. (2021-06-15). “Expression of Mucoproteins in Gallbladder Cancer”. Indian Journal of Surgery. 84 (3): 456–462. doi:10.1007/s12262-021-02989-7. ISSN 0972-2068. S2CID 235442291.
  • Clinical Significance – Mucolytic medications will break through the disulfide bonds and lower the viscosity of the mucus, thus allowing the hypersecreted mucus to be more manageable. A hypersectretion of mucus is often a symptom of pulmonary diseases or respiratory infections.
    •  “Acetylcysteine”, Meyler’s Side Effects of Drugs, Elsevier, pp. 23–25, 2016, doi:10.1016/b978-0-444-53717-1.00217-1, ISBN 9780444537164, retrieved 2022-05-06
  • There are two subgroups in mycolytic medications and each one works differently to control the hypersecreted mucus.
    • Classic mucolytic medications these medications change the disulfide bond by reducing it to a thiol bond, thus thoroughly breaking down the mucoproteins and making the mucus more manageable.
    • Peptide mucolytic medications these medications depolymerize DNA polymer and F-actin links that are present when the mucus hypersecretes. This preserves the mucins that are helpful to the epithelial tissue of the lungs.
      • Gupta, Rishab; Wadhwa, Roopma (2022), “Mucolytic Medications”, StatPearls, Treasure Island (FL): StatPearls Publishing, PMID 32644589, retrieved 2022-05-06
• MALAWISTA, I, and M SCHUBERT. “Chondromucoprotein: new extraction method and alkaline degradation.” The Journal of biological chemistry vol. 230,1 (1958): 535-44.
• An earlier study showed that from bovine nasal cartilage a product could be isolated consisting of chondroitin sulfate, an equivalent amount of inorganic cation, and protein (1). Three lines of evidence indicated that the polysaccharide and protein were combined as a mucoprotein. It will be convenient to distinguish this as bovine nasal chondromucoprotein. The extraction method was mild but tedious…The present report describes a new method for the extraction of chondromucoprotein from cartilage with a high speed homogenizer which, in a single extraction with water, can yield in 1 day an amount of apparently undegraded chondromucoprotein accounting for over 80 per cent of the total hexosamine initially present. In addition, a study of the alkaline degradation of chondromucoprotein led to a greatly simplified method for the preparation of chondroitin sulfate as a crystalline barium salt…A method is described by which the chondromucoprotein of fresh or dried beef nasal cartilage can be extracted with water in yields up to 50 per cent of the dry weight of the cartilage. This yield accounts for 80 per cent of the total hexosamine of the original cartilage. The essential feature of the method is the thorough disintegration of the cartilage in a large volume of water by the use of a high speed homogenizer. A simple method is described for degrading this chondromucoprotein to give CHONDROITIN SULFATE as the crystalline barium salt in a yield of over 75 per cent of the amount contained in the chondromucoprotein.
  • BIBLIOGRAPHY
    • Shatton, J., and Schubert, M., J. Biol. Chem., 211, 565 (1954).
    • Schloss, B., Anal. Chem., 23, 1321 (1951).
    • Einbinder, J., and Schubert, M., J. Biol. Chem., 185, 725 (1950).
    • Troll, W., and Cannan, R. K., J. Biol. Chem., 200, 803 (1953).
    • Troll, W., and Lindsley, J., J. Biol. Chem., 216, 655 (1955).
    • Glegg, R. E., Eidinger, D., and Leblond, C. P., Science, 120, 839 (1954).
    • Beaven, G. H., and Holiday, E. R., Advances in Protein Chem., 7, 319 (1952).
    • Mathews, M. B., and Losaityte, I., Federation Proc., 16, 310 (1956).
    • Hadidian, Z., and Pirie, N. W., Biochem. J., 42, 260 (1948).
    • Einbinder, J., and Schubert, M., J. Biol. Chem., 191, 591 (1951).
    • Muir, H., Biochem. J., 66, 33P (1957).
    • Jorpes, E., Biochem. Z., 204, 354 (1929).
    • Hass, G. M., and Garthwaite, B., Arch. Path., 33, 145 (1942).
• I’m going to add chondroitin wikipedia notes…A chondroitin is a chondrin derivative. Types include:
  • CHONDROITIN SULFATE
  • DERMATAN SULFATE
• Chondroitin as a supplement is now commonly used (often in combination with glucosamine) in treating the joint disease of osteoarthritis. In contrast to the symptomatic treatments, chondroitin can modify the progression of a disease process in the patient which it can be used as an alternative medicine. Chondroitin’s effect toward the articular cartilage integrity as it is part of the proteoglycan molecules. The cartilage proteoglycan synthesis can speed up as chondroitin is going through the pathway of the alimentary canal. Research has been conducted to show the effectiveness of chondroitin and results indicate that it helps to manage pain in knee and hip, slow down the progression and also recovery. However, the effectiveness of the drugs is still doubtful.
  • Chondroitin at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
  • Vasiliadis, Haris S; Tsikopoulos, Konstantinos (2017). “Glucosamine and chondroitin for the treatment of osteoarthritis”. World Journal of Orthopedics. 8 (1): 1–11. doi:10.5312/wjo.v8.i1.1. ISSN 2218-5836. PMC 5241539. PMID 28144573.
  • Tavakol, Kamran. “Glucosamine and Chondroitin for Treatment of Osteoarthritis: A Systematic Quality Assessment and Meta-analysis”. Physical Therapy. 80 (10): 1049.
  • Zhu, Xiaoyue; Sang, Lingli; Wu, Dandong; Rong, Jiesheng; Jiang, Liying (December 2018). “Effectiveness and safety of glucosamine and chondroitin for the treatment of osteoarthritis: a meta-analysis of randomized controlled trials”. Journal of Orthopaedic Surgery and Research. 13 (1): 170. doi:10.1186/s13018-018-0871-5. ISSN 1749-799X. PMC 6035477. PMID 29980200.

Methemalbumin in acute pancreatitis. Winstone NE.Br J Surg. 1965 Oct;52(10):804-8. doi: 10.1002/bjs.1800521025.PMID: 5829770 No abstract available.

Determination of methaemalbumin in plasma. Chong GC, Owen JA.J Clin Pathol. 1967 Mar;20(2):211-2. doi: 10.1136/jcp.20.2.211-b.PMID: 5602514 Free PMC article. No abstract available.

Spectral absorption characteristics of human methemalbumin. Daniels CA, Wagner C, Hartmann RC, Jenkins DE Jr.Am J Clin Pathol. 1967 May;47(5):631-7. doi: 10.1093/ajcp/47.5.631.PMID: 6022994 No abstract available.

Methemalbumin in the diagnosis of acute pancreatitis. Joseph WL, Stevens GH, Longmire WP Jr.J Surg Res. 1968 May;8(5):206-10. doi: 10.1016/0022-4804(68)90087-5.PMID: 5300526 No abstract available.

The significance of methemalbumin in fetal fluids. Halitsky V, Krumholz BA, Wiener MF.Obstet Gynecol. 1968 Sep;32(3):339-49.PMID: 5754582 No abstract available.

Assessment of methemalbumin as a diagnostic test for acute pancreatitis. Anderson MC, Toronto IR, Needleman SB, Gramatica L.Arch Surg. 1969 Jun;98(6):776-80. doi: 10.1001/archsurg.1969.01340120124023.PMID: 5768077 No abstract available.

The evolution of methaemalbumin. Baur EW.Comp Biochem Physiol. 1969 Aug 15;30(4):657-64. doi: 10.1016/0010-406x(69)92143-4.PMID: 5390337 No abstract available.

[A study of serum methemalbumin in acute pancreatitis]. Lashchevker VM.Vestn Khir Im I I Grek. 1970 Feb;104(2):51-3.PMID: 5429935 Russian. No abstract available.

[Pathological hemin pigment of the serum–methemalbumin (diagnosis of hemorrhagic pancreatitis). Review of the literature]. Lashchevker VM.Klin Khir (1962). 1970 Apr;4:89-94.PMID: 4920634 Review. Russian. No abstract available.

Amniotic fluid analysis in erythroblastosis fetalis. I. The effect of oxyhemoglobin, methemalbumin, and meconium. Halitsky V, Krumholz BA.Am J Obstet Gynecol. 1970 Apr 15;106(8):1209-13. doi: 10.1016/0002-9378(70)90519-3.PMID: 5437814 No abstract available.

Methaemalbumin in pancreatic and other diseases. Battersby C, Green MK.Gut. 1970 Nov;11(11):979.PMID: 5312806 Free PMC article. No abstract available.

[Value of methemalbumin in diagnosis and treatment of acute pancreatitis]. Kiekens R, Govaerts JP, Van Geertruyden J.Acta Gastroenterol Belg. 1971 Jan;34(1):96-104.PMID: 5087799 French. No abstract available.

The enzymatic degradation of hemoglobin to bile pigments by macrophages. Pimstone NR, Tenhunen R, Seitz PT, Marver HS, Schmid R.J Exp Med. 1971 Jun 1;133(6):1264-81. doi: 10.1084/jem.133.6.1264.PMID: 4396973 Free PMC article.

Homogenates of pure populations of these cells were devoid of heme-oxygenase activity, unless before harvesting the macrophages had been exposed to methemalbumin, microcrystalline hemin, or hemoglobin in vivo. In macrophages exposed to heme pigments, the specific activity …

The surgical significance of methaemalbuminaemia. Battersby C, Green MK.Gut. 1971 Dec;12(12):995-1000. doi: 10.1136/gut.12.12.995.PMID: 5316584 Free PMC article.

A quantitative estimation of plasma methaemalbumin can be useful. In pancreatitis, it usually indicates severe and haemorrhagic disease, and is thus of prognostic importance, as well as indicating the need for the full therapeutic regime for conservative management of the …

Methemalbumin in acute pancreatitis: an experimental and clinical appraisal. Kelly TR, Klein RL, Porquez JM, Homer GM.Ann Surg. 1972 Jan;175(1):15-8. doi: 10.1097/00000658-197201000-00003.PMID: 5060853 Free PMC article. No abstract available.

Acute pancreatitis. Geokas MC.Calif Med. 1972 Aug;117(2):25-39.PMID: 4559467 Free PMC article. Review.

Elevated serum or urinary alpha-amylase is the most important finding in diagnosis. The presence of methemalbumin in serum and in peritoneal or pleural fluid supports the diagnosis of the hemorrhagic form of the disease in patients with a history and enzyme studies suggest …

Amniocentesis and the significance of methemalbumin. Ayres RE.J Am Osteopath Assoc. 1972 Dec;72(4):385-9.PMID: 4485862 No abstract available.

[Indices of oxidation-reduction processes and methemalbumin in the blood in rheumatism in relation to the hemodynamic state]. Kruchanitsia MI.Fiziol Zh. 1973 May-Jun;19(3):411-3.PMID: 4543453 Ukrainian. No abstract available.

Measurement of methaemalbumin in plasma. Murray K, Knight M, Owen JA.J Clin Pathol. 1973 Jun;26(6):446-7. doi: 10.1136/jcp.26.6.446.PMID: 4718969 Free PMC article.

Using the method of Chong and Owen (1967), the normal range of methaemalbumin in plasma was 0 to 0.6 mg/100 ml, expressed as milligrams of haematin per cent. …

Determination of serum methemalbumin.Walberg CB, Geokas MC, Rinderknecht H.Clin Chim Acta. 1973 Oct 12;48(2):229-32. doi: 10.1016/0009-8981(73)90370-7.PMID: 4758885 No abstract available.

Methemalbumin in the diagnosis of acute hemorrhagic pancreatitis.Geokas MC, Rinderknecht H, Walberg CB, Weissman R.Ann Intern Med. 1974 Oct;81(4):483-6. doi: 10.7326/0003-4819-81-4-483.PMID: 4414654 No abstract available.

Letter: Methemalbumin and pancreatitis: a warning.Schow PD, Englert E.Ann Intern Med. 1975 Feb;82(2):281-2. doi: 10.7326/0003-4819-82-2-281.PMID: 1115454 No abstract available.

The degradative effects of porphyrins and heme compounds on components of the microsomal mixed function oxidase systemMaines MD, Kappas A.J Biol Chem. 1975 Mar 25;250(6):2363-9.PMID: 1117008 Free article.

The effects of in vitro treatment of the hepatic microsomal fraction with various porphyrin compounds on the activity and the content of the heme-containing components of the mixed function oxidase system were studied. The compounds examined were hematin, methemalbumin (wi …

A spectrophotometric scanning technique for the rapid determination of plasma hemoglobin.Blakney GB, Dinwoodie AJ.Clin Biochem. 1975 Apr;8(2):96-102. doi: 10.1016/s0009-9120(75)91005-x.PMID: 236098

The spectrophotometric scanning technique offers a rapid and accurate means of assessing plasma hemoglobin concentrations even in the presence of usual interferences by bilirubin, turbidity and methemalbumin….

Acute pancreatitis ten years experience in the Waikato district.Opie JC.N Z Med J. 1975 Aug 27;82(546):126-9.PMID: 1081208

Abnormal biochemistry is discussed and related to mortality. Additional tests, serum catalase/methaemalbumin are promoted to assist in the diagnosis and indicating the severity of the disease. …

Clinical determination of methemalbumin.Andres SF, Kregoski TJ, Frey CF, Joseph RR, McCann DS.Clin Chem. 1975 Sep;21(10):1506-10.PMID: 1157322

Results may be expressed either in terms of micrograms of methemalbumin iron per gram of albumin or in milligrams of methemalbumin per liter. The reproducibility of the method is of the order of +/- 7% (SD). Normal persons have essentially no methemalbumin ir …

[Methemalbumin as a possible prognostic test after laparotomy].Giler S, Abu-Dalu J, Urca I.Med Chir Dig. 1976;5(3):153-6.PMID: 1004045 French. No abstract available.

Interaction of serum albumin with normal and sickle hemoglobins.Adachi K, Asakura T.Biochim Biophys Acta. 1976 Apr 14;427(2):536-48. doi: 10.1016/0005-2795(76)90196-3.PMID: 5129

After purification by Sephadex G-200 column chromatography, this compound was identified as methemalbumin. Comparison of the rates of formation of methemalbumin from hemoglobin with various ligand states and human serum albumin showed that the rate of formation from … 

Determination of bilirubin in amniotic fluid: a comparison of some current methods.

Simkins A, Worth HG.Ann Clin Biochem. 1976 Sep;13(5):510-5. doi: 10.1177/000456327601300151.PMID: 1033728

The within-batch precision and recovery of bilirubin by each method has been studied, and the effects of the presence of haemoglobin, methaemoglobin, and methaemalbumin in varying concentrations have been investigated. Measurements on amniotic fluid from patients with rhes …

Transfer of heme from heme-albumin to hemopexin.Morgan WT, Liem HH, Sutor RP, Muller-Ebergard U.Biochim Biophys Acta. 1976 Sep 24;444(2):435-45. doi: 10.1016/0304-4165(76)90387-1.PMID: 971416

However, more than 80% of heme was transferred from methemalbumin to hemopexin within 24 h. Heme added to a 1 : 70 mixture of the apo-proteins is initially bound by albumin; but more than 90% is bound by hemopexin in 24 h. Addition of dithionite causes nearly all of the he …

Diagnosis of intravascular haemolysis using starch gel electrophoresis.Ali MA, Vanderlinder B.Scand J Haematol. 1977 Oct;19(4):343-6. doi: 10.1111/j.1600-0609.1977.tb01484.x.PMID: 72403

Decreased serum haptoglobins, increased free plasma haemoglobin and the presence of methaemalbumin was documented in all 15 patients. It is suggested that starch gel electrophoresis and staining with haem specific stain is a simple and reliable method for confirming intrav …

Differential spectrophotometry for the determination of serum methemalbumin.Kempisty CC, Zak B.Clin Chim Acta. 1977 Oct 1;80(1):187-92. doi: 10.1016/0009-8981(77)90278-9.PMID: 908141

A method for the direct determination of methemalbumin in serum by means of difference scanning spectrophotometry is described. …

Haptoglobins in some Galliformes birds.Musquera S, Planas J.Poult Sci. 1977 Nov;56(6):1777-82. doi: 10.3382/ps.0561777.PMID: 611487 Free article.

Paper, acetate and starch-gel electrophoresis were carried out and two benzidine stain regions were observed when haemoglobin (Hb) was added to normal plasma (Hb-Hp complex and methemalbumin) besides the free Hb when the binding capacity of these proteins were surpassed. . … 

Hemorrhagic shock: concept of toxic elements.Friedman-Mor Z, Chalon J, Turndorf H, Orkin LR.N Y State J Med. 1977 Dec;77(14):2217-9.PMID: 271777 No abstract available.

Evaluation of methaemalbumin in acute pancreatitis.Lankisch PG, Koop H, Otto J, Oberdieck U.Scand J Gastroenterol. 1978;13(8):975-8. doi: 10.3109/00365527809181378.PMID: 725521

Methaemalbumin (MHA) was measured in 62 patients with acute pancreatitis. … 

[Methemalbumin: a further diagnostic and prognostic parameter in acute pancreatitis].Lankisch PG, Koop H, Otto J, Oberdieck U.Verh Dtsch Ges Inn Med. 1978;(84):1046-9.PMID: 741874 German. No abstract available.

A new method for determination of microsomal haem oxygenase (EC 1.14.99.3) based on quantitation of carbon monoxide formation. Cavallin-Ståhl E, Jönsson GI, Lundh B.Scand J Clin Lab Invest. 1978 Feb;38(1):69-76. doi: 10.3109/00365517809108405.PMID: 24261

In the present study a method is described where MHO activity is assessed from the rate of carbon monoxide formation (VCO) in tissue homogenates with methaemalbumin as substrate. Formed CO is bound to haemoglobin present in the tissue homogenate. …

Studies on the ascites fluid of acute pancreatitis in man. Geokas MC, Rinderknecht H, Brodrick JW, Largman C.Am J Dig Dis. 1978 Feb;23(2):182-8. doi: 10.1007/BF01073198.PMID: 623083

In eight patients methemalbumin was detected in ascites and serum, supporting the diagnosis of hemorrhagic pancreatitis. …Substantial amounts of alpha2-macroglobulin-bound trypsin and elastase 2, entering the circulation from the peritoneal cavity, might be responsible f …

Abnormal heme-protein patterns in hemorrhagic shock. Friedman-Mor Z, Chalon J, Gorstein F, Turndorf H, Chuba JV, Orkin LR.J Trauma. 1978 Feb;18(2):104-7. doi: 10.1097/00005373-197802000-00005.PMID: 633416

As shock deepened and mortality rose, hemopexin-heme and methemalbumin appeared. The highest mortality rate (4 out of 5 cases) was found when both hemopexin-heme and methemalbumin were present. …

Cerebral arterial spasm. II. Etiology and treatment of experimental cerebral vasospasm. Morooka H.Acta Med Okayama. 1978 Apr;32(1):39-49.PMID: 207156

Spasmogenic substances which contained a heme component, such as methemoglobin, methemalbumin and catalase enhanced DBH activity in human serum as measured by a one step chemical spectrophotometric assay. The concentration which gave the highest DBH activity caused the max …

Methaemalbuminaemia as a cause of cyanosis.Drysdale HC, Hunton J.Br Med J. 1978 Apr 15;1(6118):962. doi: 10.1136/bmj.1.6118.962.PMID: 638552 Free PMC article. No abstract available. 

Determination of plasma or serum haemoglobin by peroxidase activity employing 2,2′-azino-di-(3-ethyl-benzthiazolinsulphonate-6) as chromogen.Marklund S.Scand J Clin Lab Invest. 1978 Oct;38(6):543-7. doi: 10.1080/00365517809108817.PMID: 30165

The peroxidase activity of myoglobin is similar to that of haemoglobin, whereas haemin in free solution, bound to haemopexin or to albumin (methaemalbumin) shows much lower activity. The precision within run is satisfactory, +/- 5%….

[Heme binding by the serum proteins of fish].Giebelmann R, Garske R, Gibb B.Arch Exp Veterinarmed. 1979 Jan;33(1):21-6.PMID: 454088 German.

Hemorrhagic pancreatitis. Frey CF.Am J Surg. 1979 May;137(5):616-23. doi: 10.1016/0002-9610(79)90034-5.PMID: 453456

Early recognition of patients with hemorrhagic pancreatitis can be facilitated by the routine use of amylase and methemalbumin determinations and peritoneal lavage. Translocation of large volumes of albumin-rich fluid from the intravascular compartment to the retroperitone …

Degradation of cytochrome P-450 haem by carbon tetrachloride and 2-allyl-2-isopropylacetamide in rat liver in vivo and in vitro. Involvement of non-carbon monoxide-forming mechanisms. Guzelian PS, Swisher RW.Biochem J. 1979 Dec 15;184(3):481-9. doi: 10.1042/bj1840481.PMID: 120199 Free PMC article.

By comparison with breakdown of intrinsic haem, catabolism of [(14)C]methaemalbumin by microsomal haem oxygenase in vitro produced equimolar quantities of (14)CO and bilirubin, although these catabolites reflected only 18% of the degraded [(14)C]haem. …

A compaative study of methods for the prediction of severity of attacks of acute pancreatitis. McMahon MJ, Playforth MJ, Pickford IR.Br J Surg. 1980 Jan;67(1):22-5. doi: 10.1002/bjs.1800670107.PMID: 7357236

By 48 h after admission clinical assessment was comparable to systems using multiple criteria in its predictive value, success rates being 83 per cent and 82 per cent respectively for prediction of severe attacks. We did not find either the presence of methaemalbumin in th …

[Methemalbulinemia: usefulness and specificity of its determination in the prognosis of acute pancreatitis]. Borda F, Gamboa J, Uribarrena R, Rivero Puente A.Rev Esp Enferm Apar Dig. 1980 Jul;58(1):35-40.PMID: 7403632 Spanish. No abstract available.

Kinetics and mechanism of the interaction between human serum albumin and monomeric haemin. Adams PA, Berman MC.Biochem J. 1980 Oct 1;191(1):95-102. doi: 10.1042/bj1910095.PMID: 7470101 Free PMC article.

The results obtained under conditions of albumin saturation of haemin and under pseudo-single turnover conditions indicate that methaemalbumin is formed in a two-stage, single-intermediate process. …

Haem-binding proteins in Gallus domesticus. Csuka J.Folia Biol (Praha). 1981;27(1):37-40.PMID: 7202766

Three electrophoretically different forms of the haem-binding proteins were observed. The permanent haem-binding protein (methaemalbumin) occurred from hatching until late age and was present in the blood serum and in some other organs. …

The cytochromes in microsomal fractions of germinating mung beans. Hendry GA, Houghton JD, Jones OT.Biochem J. 1981 Mar 15;194(3):743-51. doi: 10.1042/bj1940743.PMID: 7306021 Free PMC article.

Of the b cytochromes, cytochrome b5 alone undergoes a rapid degradation after day 4, Changes in cytochrome b concentrations were confined to the microsomal fraction: mitochondrial b cytochrome concentrations were unaltered with age. Protohaem degradation (of exogenous methaema …

Mechanisms of hemolysis. Zipursky A.Mead Johnson Symp Perinat Dev Med. 1982;(19):17-24.PMID: 6821465 Review.

Determination of serum methemalbumin by second-derivative spectroscopy. Bertrand A, Cox C, Foucart P, Buret J.Clin Chim Acta. 1982 Aug 4;123(1-2):121-6. doi: 10.1016/0009-8981(82)90120-6.PMID: 7116634 No abstract available.

Effect of iron and hemoproteins on hydrogen peroxide-supported styrene oxidation to styrene oxide. Cantoni L, Blezza D, Belvedere G.Experientia. 1982 Oct 15;38(10):1192-4. doi: 10.1007/BF01959735.PMID: 6814946 No abstract available.

Uptake and degradation of proteins by isolated rat liver lysosomes. Suggestion of a microautophagic pathway of proteolysis.

Ahlberg J, Marzella L, Glaumann H.Lab Invest. 1982 Dec;47(6):523-32.PMID: 6755063

Heme oxygenase activity in liver microsomes of the bullfrog, Rana catesbeiana, and its change during the tadpole development. Okazaki T, Watanabe E, Suzuki S, Shukuya R.Comp Biochem Physiol B. 1983;74(3):533-7. doi: 10.1016/0305-0491(83)90224-9.PMID: 6601562

The specific activity of the enzyme of premetamorphic tadpole liver was comparable to that of adult frog liver. The apparent Km for methemalbumin was about 25 microM for both tadpole and frog enzymes. 2. The enzyme activity was stimulated in vivo by the injection of met …

[Voluntary poisoning by intravenous injection of caustic soda]. Blin F, Rochette J, Taulet G, Pellerin M, Marsepoil T, Starkman M.Ann Fr Anesth Reanim. 1983;2(2):97-9. doi: 10.1016/s0750-7658(83)80010-0.PMID: 6625254 French.

[Differential diagnostic and prognostic value of methemalbumin determination in acute pancreatitis]. Bornschein W.Leber Magen Darm. 1983 Mar;13(2):64-6.PMID: 6621243 German.

Methemalbumin (MHA) levels of serum or ascites were measured in a prospective study involving 40 patients with acute abdominal symptomatology suspected to have acute pancreatitis. ..

[Methemalbuminemia (pseudomethemoglobinemia): a cause of cyanosis with normal arterial oxygen pressure. Role of massive hemolysis]. Blin F, Taulet G, Marsepoil T, Rochette J.Presse Med. 1984 Jan 14;13(1):48-9.PMID: 6231555 French. No abstract available.

Investigation of the haemolytic effects of Ancylostoma ceylanicum: observations on infected dogs in vivo and human and dog blood in vitro. Carroll SM, Pryor J, Kennett DW, Grove DI.Southeast Asian J Trop Med Public Health. 1984 Mar;15(1):129-34.PMID: 6740375

Erythrocytes from the latter animals showed significantly greater autohaemolysis in the presence of added glucose. When serum bilirubin and methaemalbumin, plasma haemoglobin, urinary urobilinogin and osmotic fragility of their red cells were measured, however, no evidence …

Current concepts. Diagnostic tests and procedures in acute pancreatitis. Moossa AR.N Engl J Med. 1984 Sep 6;311(10):639-43. doi: 10.1056/NEJM198409063111005.PMID: 6472342 No abstract available. 

The presence of a meconium-like substance in second-trimester amniotic fluid. Alger LS, Kisner HJ, Nagey DA.Am J Obstet Gynecol. 1984 Oct 15;150(4):380-5. doi: 10.1016/s0002-9378(84)80143-x.PMID: 6486201

Methene bridge carbon atom elimination in oxidative heme degradation catalyzed by heme oxygenase and NADPH-cytochrome P-450 reductase. Docherty JC, Firneisz GD, Schacter BA.Arch Biochem Biophys. 1984 Dec;235(2):657-64. doi: 10.1016/0003-9861(84)90241-8.PMID: 6440489

The enzymatic mechanisms of heme degradation in the presence and absence of heme oxygenase were compared by analyzing the recovery of 14CO from the degradation of [14C]heme. 14CO recovery from purified NADPH-cytochrome P-450 reductase-catalyzed degradation of [14C]methemalbumi …

Percutaneous peritoneal dialysis as an early treatment of acute necrotic hemorrhagic pancreatitis. Reynaert MS, Bshouty ZH, Otte JB, Kestens PJ, Tremouroux J.Intensive Care Med. 1985;11(3):123-8. doi: 10.1007/BF00258536.PMID: 3998272

The introduction of methemalbuminemia and the presence of specific ascites rich in amylase, lipase and methemalbumin as early indicators of the presence of ANHP enabled us to confirm the diagnosis in 53 patients, after which early institution of PPD was possible. …

A characterization of haem uptake and intracellular distribution by isolated hepatocytes. Lodola A.Biosci Rep. 1985 Jul;5(7):609-14. doi: 10.1007/BF01117074.PMID: 4041567

An increase in cell haem content occurred after a challenge with 5, 10 or 20 microM haem, supplied as methaemalbumin. The rate of haem uptake was temperature dependent; no non-specific binding occurred. …

Diagnosis of acute pancreatitis. Lempinen M, Schröder T.Eur Surg Res. 1986;18(3-4):230-7. doi: 10.1159/000128531.PMID: 2424764

Quantitative determination of serum methemalbumin. Cowley DM, Powell VR.Pathology. 1986 Jul;18(3):310-2. doi: 10.3109/00313028609059482.PMID: 3785980

We have devised a simple, sensitive quantitative method for the determination of serum methemalbumin. The method uses a modified Allen correction to correct the alpha band of methemalbumin at 623 nm for background turbidity. …

Pancreatitis causes brownish-black peritoneal dialysate due to the presence of methaemalbumin. Connacher AA, Stewart WK.Nephrol Dial Transplant. 1987;2(1):45-7.PMID: 3110698

Examination of the second patient’s dialysate by recording spectrophotometry showed the discoloration to be due to methaemalbumin. Haemorrhagic pancreatitis, by releasing proteolytic enzymes and perhaps by using the lesser sac as an anatomical cul-de-sac for chemical react …

Biochemical studies in peritoneal fluid from patients with acute pancreatitis. Relationship to etiology. Dubick MA, Mayer AD, Majumdar AP, Mar G, McMahon MJ, Geokas MC.Dig Dis Sci. 1987 Mar;32(3):305-12. doi: 10.1007/BF01297058.PMID: 3816484

Coupled with elevated ribonuclease activity (27.4 +/- 3.4 units), a positive methemalbumin test in 23 of 24 patients (1.1 +/- 0.4 mg hematin/100 ml), and an average protein concentration of 4.0 +/- 0.2 g/100 ml, these observations demonstrate that abdominal paracentesis an …

[Laboratory diagnosis of acute pancreatitis]. Bode JC, Bode C.Dtsch Med Wochenschr. 1987 Jul 31;112(31-32):1220-2. doi: 10.1055/s-2008-1068226.PMID: 2440657 German. No abstract available

Haem arginate: a new stable haem compound. Tenhunen R, Tokola O, Lindén IB.J Pharm Pharmacol. 1987 Oct;39(10):780-6. doi: 10.1111/j.2042-7158.1987.tb05119.x.PMID: 2891815

Haem arginate and haem lysinate were found to be as good substrates as methaemalbumin. Stock solutions of haem arginate were stable for 2 years at +6 degrees C. …

Heme oxygenase activity as measured by carbon monoxide production. Vreman HJ, Stevenson DK.Anal Biochem. 1988 Jan;168(1):31-8. doi: 10.1016/0003-2697(88)90006-1.PMID: 3364715

Tissue homogenates were centrifuged and the 13,000g supernatants were incubated in septum-sealed vials with methemalbumin in the presence and absence of NADPH at 37 degrees C for 15 min. …

Methemalbumin: reality and myth. George JW.Vet Clin Pathol. 1988;17(2):43-6. doi: 10.1111/j.1939-165x.1988.tb00489.x.PMID: 15162335

Actually, only albumin from a limited number of species makes methemalbumin. Lack of recognition of interspecies differences has led to the myth of methemalbumin in domestic animals. The literature on methemalbumin is reviewed including the chemical character …

The mechanism of the suicidal, reductive inactivation of microsomal cytochrome P-450 by carbon tetrachloride. Manno M, De Matteis F, King LJ.Biochem Pharmacol. 1988 May 15;37(10):1981-90. doi: 10.1016/0006-2952(88)90545-x.PMID: 3377806

A rapid destruction of haem was also observed during the non-enzymatic reductive incubation of CCl4 with soluble haem preparations (methaemalbumin) in presence of sodium dithionite. The results indicate that haem is both the site and the target of the suicidal activation o …

Pharmacological and biological effects of tin-protoporphyrin on neonatal hyperbilirubinemic Gunn rats. Nagae H, Keino H, Watanabe K, Kashiwamata S.Pediatr Res. 1988 Aug;24(2):209-12. doi: 10.1203/00006450-198808000-00014.PMID: 3186333

Methemalbumin interference in various biochemical assays of plasma. Myara I, Myara A, Drupt F, Moatti N, Trivin F.Clin Chem. 1988 Sep;34(9):1919-20.PMID: 3416455 No abstract available.

The degradation of haem by carbon tetrachloride: metabolic activation requires a free axial coordination site on the haem iron and electron donation. Manno M, King LJ, De Matteis F.Xenobiotica. 1989 Sep;19(9):1023-35. doi: 10.3109/00498258909043159.PMID: 2510407

The ability of haem to catalyse the reductive activation of carbon tetrachloride (CCl4) in vitro has been investigated under anaerobic conditions, using methaemalbumin (MHA) and either sodium dithionite or NADPH together with NADPH-cytochrome P-450 reductase (EC 1.6.2.4) a …

Three direct spectrophotometric methods for determination of total bilirubin in neonatal and adult serum, adapted to the Technicon RA-1000 analyzer. Harrison SP, Barlow IM.Clin Chem. 1989 Sep;35(9):1980-6.PMID: 2776331

All methods required only 10 microL of serum, were precise (between-batch CVs less than 4.2%, analyte range 64-310 mumol/L), linear to 1000 mumol/L, and insensitive to interference from hemoglobin to 5 g/L. Lipemia, carotene, and methemalbumin interfered positively with ea …

Heme-peptide/protein interactions: the binding of heme octa and undecapeptides, and microperoxidase-8 and -11, to human serum albumin. Adams PA, Goold RD, Thumser AA.J Inorg Biochem. 1989 Oct;37(2):91-103. doi: 10.1016/0162-0134(89)80032-7.PMID: 2557388

CN- binding has been used to investigate the accessibility of the heme in each complex by comparison with CN- interaction with methemalbumin. A preliminary study of the kinetics of the Fe3+MP-8/11 human serumalbumin (HSA) interaction demonstrates a clear ligand-size-relate …

Methemalbumin in acute pancreatitis: an evaluation of its prognostic value and comparison with multiple prognostic parameters. Lankisch PG, Schirren CA, Otto J.Am J Gastroenterol. 1989 Nov;84(11):1391-5.PMID: 2816872

Methemalbumin (MHA) and Ranson’s prognostic parameters were measured in 90 patients with acute pancreatitis. …

[Hemolytic anemia in patients with heart valve prosthesis]. Cervantes Escárcega JL, Izaguirre Avila R.Arch Inst Cardiol Mex. 1990 Mar-Apr;60(2):201-8.PMID: 2198853 Review. Spanish.

We describe the value of the quantitation of unconjugated bilirubin, free plasmatic hemoglobin, DHL and it’s DHL1 iso enzyme, methemalbumin and urinary hemosiderin for the specific diagnosis of this entity. …

Inhibition of human lymphocyte ferrochelatase activity by hemin. Rossi E, Attwood PV, Garcia-Webb P, Costin KA.Biochim Biophys Acta. 1990 May 8;1038(3):375-81. doi: 10.1016/0167-4838(90)90251-a.PMID: 2340297

Human serum albumin, but not bovine serum albumin was able to prevent the inhibition consistent with the known formation of the tightly bound methemalbumin complex with human albumin. Kinetic studies performed under initial velocity conditions with hemin concentrations ran …

Correlation of pancreatic enzyme levels with the patient’s recovery from acute edematous pancreatitis. Lankisch PG, Buschmann-Kaspari H, Otto J, Schröder K, Koop H.Klin Wochenschr. 1990 Jun 5;68(11):565-9. doi: 10.1007/BF01667149.PMID: 1695957

Levels of total amylase, pancreatic isoamylase, lipase and immunoreactive trypsin were measured in 27 consecutive patients with noncomplicated (methemalbumin negative, no pseudocysts on ultrasound) acute pancreatitis during the acute, subacute, and recovery phases. …

Increased body weight as a prognostic parameter for complications in the course of acute pancreatitis. Lankisch PG, Schirren CA.Pancreas. 1990 Sep;5(5):626-9. doi: 10.1097/00006676-199009000-00021.PMID: 2235973

To evaluate whether obesity is a negative prognostic parameter in the course of acute pancreatitis, we examined 149 patients and divided them into four weight groups. Single (methemalbumin) and multiple (Ranson’s signs) prognostic parameters were found to be independent of …

[The simultaneous establishment of the presence and classification of human (primate), bird and fish blood by electrophoresis]. Il’ina EA.Sud Med Ekspert. 1991 Jan-Mar;34(1):28-32.PMID: 1858114 Russian.

Method is based on detection of diagnostical characteristics possessing specific electrophoretic mobility and doesn’t require use of precipitating sera. Diagnostical feature of human (primates) blood is methaemalbumin detectable in anodic part of electrophoregram and not d … 

[Comparison of 2 methemalbumin measurements and of free hemoglobin in view of their value in the differential diagnosis of edematous and hemorrhagic pancreatitis]. Bosseckert H, Günther E, Striehn E, Felsch B, Erler M, Sallen B.Gastroenterol J. 1991;51(1):1-4.PMID: 1910488 German.

Methemalbumin was estimated by means of electrophoresis and the method of Walberg, respectively in 59 patients (25 pat. with edematous, 34 patients with hemorrhagic pancreatitis) partially over three days. In addition the estimation of free hemoglobin in ascites has been c …

Methods for measuring plasma hemoglobin in micromolar concentration compared. Fairbanks VF, Ziesmer SC, O’Brien PC.Clin Chem. 1992 Jan;38(1):132-40.PMID: 1733585

Of eight methods examined for measuring plasma hemoglobin in micromolar concentration, all exhibited acceptable linearity, reproducibility, and concurrence except when specimens were icteric or lipemic or contained methemoglobin or methemalbumin. Measurement of absorbance …

[Autoimmune hemolytic anemia with medullary erythroplasia and black serum with methemalbuminemia]. Betticher DC, Pugin P.Schweiz Med Wochenschr. 1993 Jan 23;123(3):67-9.PMID: 8426949 French.

On day 5 of therapy with corticosteroids, immunoglobulin and several plasmaphereses, a high level of methemalbumin was found while RBC lysis had ceased and bilirubin and haptoglobin had normalized. …The patient has not had any other hemolytic crisis without treatment (fo …

Discolouration of skin and serum after sweet ingestion. Anttila VJ, Mäkelä JP, Soininen K, Helminen V, Tenhunen R.Lancet. 1993 Jun 5;341(8858):1476-7. doi: 10.1016/0140-6736(93)90919-8.PMID: 8099167 No abstract available.

The stability of the heme-globin linkage: measurement of heme exchange. Benesch RE.Methods Enzymol. 1994;231:496-502. doi: 10.1016/0076-6879(94)31033-5.PMID: 8041271 No abstract available.

Reductive activation of halothane by human haemoglobin results in the modification of the prosthetic haem. Tolando R, Cazzaro S, Ferrara R, Rezzadore M, Manno M.Biochem Pharmacol. 1995 Jan 18;49(2):233-41. doi: 10.1016/s0006-2952(94)00402-1.PMID: 7840801

Reductive halothane metabolism was observed under these conditions as indicated by a dose-dependent inorganic fluoride (F-) production, which was, however, lower than that observed with heated Hb or a water soluble haem preparation (methaemalbumin). A rapid, partial loss o …

Preparative and analytical applications of immobilized haemoglobin. Chiancone E, Gattoni M, Fronticelli C, Termine A, Silvestri L, Sarti P.J Chromatogr B Biomed Appl. 1995 Feb 3;664(1):89-95. doi: 10.1016/0378-4347(94)00405-t.PMID: 7757245

This reaction is difficult to study in solution owing to the overlap of the met-haemoglobin and methaemalbumin spectra….

Suicidal inactivation of haemoproteins by reductive metabolites of halomethanes: a structure-activity relationship study. Manno M, Tolando R, Ferrara R, Rezzadore M, Cazzaro S.Toxicology. 1995 Jun 26;100(1-3):175-83. doi: 10.1016/0300-483x(95)03083-r.PMID: 7624875

Human haemoglobin (Hb), methaemalbumin (MHA) or rat liver microsomal cytochrome P-450 (P-450) were incubated anaerobically at microM concentrations with 1 mM carbon tetrachloride (CCl4), trichlorobromomethane (CCl3Br), chloroform (CHCl3) or methylene chloride (CH2Cl2) in p …

[Methemalbumin–a biocatalyst of aromatic amine oxidation by hydrogen peroxide]. Rus’ OB, Puchkaev AV, Metelitsa DI.Biokhimiia. 1996 Oct;61(10):1813-24.PMID: 9011230 Russian.

The kinetics of hydrogen peroxide-dependent oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and o-phenylenediamine catalyzed by hemin and hemin-bovine serum albumin (BSA) complex (methemalbumin) was studied in buffered physiological solution containing dimethylformamide …

Inhibition of oxidation of aromatic amines in heme-containing hydrogen peroxide systems by substituted 4,6-di-tert-butyl-pyrocatechols. Metelitza DI, Rus OB, Puchkaev AV, Shadyro OI.Biochemistry (Mosc). 1997 Mar;62(3):275-84.PMID: 9275300

The substituted pyrocatechols sodium (2,3-dihydroxy-4,6-di-tert-butyl-phenyl)-S-thiosulfate (InH1) and 3-(S-glutathionyl)-4,6-di-tert-butyl-pyrocatechol (InH2) effectively inhibited oxidation of o-phenyle-nediamine and tetramethylbenzidine (TMB) catalyzed by hemin or its complex …

Hemozoin stability and dormant induction of heme oxygenase in hemozoin-fed human monocytes. Schwarzer E, De Matteis F, Giribaldi G, Ulliers D, Valente E, Arese P.Mol Biochem Parasitol. 1999 May 15;100(1):61-72. doi: 10.1016/s0166-6851(99)00031-6.PMID: 10376994

[Determining role of media in peroxide oxidation of aromatic type antioxidants with participation of methemalbumins]. Rus’ OB, Puchkaev AV, Metelitsa DI.Prikl Biokhim Mikrobiol. 2000 Mar-Apr;36(2):143-52.PMID: 10780000 Russian.

Hematin is one of the cytotoxic factors in pancreatitis-associated ascitic fluid that causes hepatocellular injury. Ueda T, Takeyama Y, Takase K, Hori Y, Kuroda Y, Ho HS.Surgery. 2002 Jan;131(1):66-74. doi: 10.1067/msy.2002.118317.PMID: 11812965

Spectroscopic studies on human serum albumin and methemalbumin: optical, steady-state, and picosecond time-resolved fluorescence studies, and kinetics of substrate oxidation by methemalbumin. Kamal JK, Behere DV.J Biol Inorg Chem. 2002 Mar;7(3):273-83. doi: 10.1007/s007750100294. Epub 2001 Oct 2.PMID: 11935351

The nature of the heme environment in methemalbumin, the Fe(III) protoporphyrin IX (heme)-human serum albumin (HSA) complex, was investigated by optical spectroscopy. …From the time-resolved fluorescence measurements and the energy transfer calculations on methemalbum …

The atomic structure of human methemalbumin at 1.9 A. Wardell M, Wang Z, Ho JX, Robert J, Ruker F, Ruble J, Carter DC.Biochem Biophys Res Commun. 2002 Mar 8;291(4):813-9. doi: 10.1006/bbrc.2002.6540.PMID: 11866438

Effects of hypoxia on heme oxygenase expression in human chorionic villi explants and immortalized trophoblast cells. Appleton SD, Marks GS, Nakatsu K, Brien JF, Smith GN, Graham CH, Lash GE.Am J Physiol Heart Circ Physiol. 2003 Mar;284(3):H853-8. doi: 10.1152/ajpheart.00655.2002.PMID: 12578814 Free article.

[Methemalbuminnemia after massive hemolysis during blackwater fever]. Gidenne S, Ceppa F, Bruneel F, Mérat S, Carde A, Brinquin L, Burnat P.Ann Biol Clin (Paris). 2003 May-Jun;61(3):332-6.PMID: 12805012 Free article. French.

We report a case of blackwater fever with brown plasma due to the presence of methemalbumin. The discovery of plasma with this color is a rare event at the laboratory. …In our case, methemalbumin was detected and quantified using a scanning spectrophotometer. ..

Reductive activation of HCFC-123 by methaemalbumin. Zanovello A, Ferrara R, Manno M.Toxicol Lett. 2003 Sep 15;144(1):127-36. doi: 10.1016/s0378-4274(03)00232-7.PMID: 12919730

Previous work in our laboratory has shown that some haloalkanes, including halomethanes CCl4, CCl3Br, CHCl4 and CH2Cl2 as well as halothane, are activated by different haemoproteins to reactive metabolites resulting in the protein’s suicidal inactivation. Among these is methae …

Effect of glucose and oxygen deprivation on heme oxygenase expression in human chorionic villi explants and immortalized trophoblast cells. Appleton SD, Lash GE, Marks GS, Nakatsu K, Brien JF, Smith GN, Graham CH.Am J Physiol Regul Integr Comp Physiol. 2003 Dec;285(6):R1453-60. doi: 10.1152/ajpregu.00234.2003.PMID: 14615405 Free article.

Although hypoxia induces heme oxygenase (HO)-1 mRNA and protein expression in many cell types, recent studies in our laboratory using human placental tissue have shown that a preexposure to hypoxia does not affect subsequent HO enzymatic activity for optimized assay conditions (2 …

The role of RBC destruction in vascular regions with high turbulence on atherosclerosis. Olgun A, Akman S, Erbil MK.Med Hypotheses. 2004;63(2):283-4. doi: 10.1016/j.mehy.2004.02.034.PMID: 15236792

As a very simple model of this possible very local event we analysed free hemoglobin and methemalbumin levels after blood collection into evacuated tubes, and found that the increase of methemalbumin is more significant than free hemoglobin. The measurement of free … 

Binding of heme to human serum albumin: steady-state fluorescence, circular dichroism and optical difference spectroscopic studies. Kamal JK, Behere DV.Indian J Biochem Biophys. 2005 Feb;42(1):7-12.PMID: 23923575

The source of endogenous carbon monoxide formation in human placental chorionic villi. Ahmed H, McLaughlin BE, Soong J, Marks GS, Brien JF, Nakatsu K.Cell Mol Biol (Noisy-le-grand). 2005 Oct 3;51(5):447-51.PMID: 16309566

Chorionic villi segments were prepared for measurements of CO production in the absence/presence of an exogenous supply of heme substrate (methemalbumin), inhibitors of HO, or inhibitors of lipid peroxidation. …The lipid peroxidation inhibitors used were EDTA (0.1 mM, 0. …

Effect of prototypic drugs ibuprofen and warfarin on global chaotropic unfolding of human serum heme-albumin: a fast-field-cycling 1H-NMR relaxometric study. Fanali G, Ascenzi P, Fasano M.Biophys Chem. 2007 Aug;129(1):29-35. doi: 10.1016/j.bpc.2007.05.002. Epub 2007 May 10.PMID: 17531369

[Substituted aminophenols and flavonoids as potential components for test-systems of total antioxidant activity]. Grigorenko IuA, Karaseva EI, Metelitsa DI, Sorokin VL, Ksendzova GA, Shadyro OI.Biomed Khim. 2007 Sep-Oct;53(5):566-76.PMID: 18078071 Russian.

A comparative kinetic study of ortho-phenylenediamine (PDA) oxidation in the “pseudoperoxidase” system Methemalbumin-H2O2 in the presence of 2-amino-4-tret-butylphenol (ATBP), 2-amino-4,6-di-tret-butylphenol (ADTBP) and its four N-acyl derivates, as well as flavonoids (que … 

Inhibition of heme oxygenase activity in newborn mice by azalanstat. Morisawa T, Wong RJ, Bhutani VK, Vreman HJ, Stevenson DK.Can J Physiol Pharmacol. 2008 Oct;86(10):651-9. doi: 10.1139/y08-069.PMID: 18841169

Purification and viral inactivation of hemoglobin from human placenta blood. Li F, Zhang H, Wang J, Yang C.Artif Cells Blood Substit Immobil Biotechnol. 2009;37(2):57-60. doi: 10.1080/10731190902742547. Epub 2009 Feb 18.PMID: 19229697 

[Highly effective test-system for determination of the total antioxidant activity of human blood serum]. Grigorenko IuA, Metelitsa DI, Piven’ NV, Lukhverchik LN, Shadyro OI.Biomed Khim. 2009 May-Jun;55(3):350-60.PMID: 19663008 Russian.

A highly effective test-system for quantitative characterization of the total antioxidant activity (TAA) of human blood serum (HBS), including methemalbumin (MetHa) as biocatalyst, H2O2 as the oxidant, o-phenylenediamine (PDA) as the acceptor of radicals and 2,2,5,7,8-pent …

HmuY haemophore and gingipain proteases constitute a unique syntrophic system of haem acquisition by Porphyromonas gingivalis. Smalley JW, Byrne DP, Birss AJ, Wojtowicz H, Sroka A, Potempa J, Olczak T.PLoS One. 2011 Feb 17;6(2):e17182. doi: 10.1371/journal.pone.0017182.PMID: 21390208 Free PMC article.

This is the first demonstration of a haemophore working in conjunction with proteases to acquire haem from haemoglobin. In addition, HmuY was able to extract haem from methaemalbumin, and could bind haem, either free in solution or from methaemoglobin, even in the presence …

[Antioxidant activity of oxygen-containing aromatic compounds]. Potapovich MV, Kurchenko VP, Metelitsa DI, Shadyro OI.Prikl Biokhim Mikrobiol. 2011 Jul-Aug;47(4):386-96.PMID: 21950111 Russian.

Inhibition efficiency (antioxidant activity) of 26 oxygen-containing aromatic compounds was studied in methemalbumin-H202-o-phenylenediamine (PDA) or tetramethylbenzidine (TMB) pseudoperoxidase system at 20 degrees C in buffered physiological solution (pH 7.4) containing 6 …

Methaemalbumin formation in sickle cell disease: effect on oxidative protein modification and HO-1 induction. Hanson MS, Piknova B, Keszler A, Diers AR, Wang X, Gladwin MT, Hillery CA, Hogg N.Br J Haematol. 2011 Aug;154(4):502-11. doi: 10.1111/j.1365-2141.2011.08738.x. Epub 2011 May 20.PMID: 21595649 Free PMC article.

In addition, methaemoglobin (metHb) releases the haem moiety, which can bind to albumin to form methaemalbumin (metHSA). This study used electron paramagnetic resonance to detect metHSA in SCD plasma and demonstrated that haptoglobin prevents haem transfer from metHb to HS …

Cola-colored dialysate as a gastrointestinal sequelae of cardiac surgery in a patient who underwent peritoneal dialysis. Ling EK, Lin BS, Chiang SS, Tsai MH.Ren Fail. 2012;34(9):1160-2. doi: 10.3109/0886022X.2012.711731. Epub 2012 Aug 14.PMID: 22888910

The findings of the dialysate analysis and the abdominal computed tomography showed that this discoloration could be due to the presence of methemalbumin caused by pancreatitis (not hemorrhagic) combined with intra-abdominal bleeding-both of which are rare gastrointestinal …

[Antioxidant activity of hydroxy derivatives of coumarin]. Potapovich MV, Metelitsa DI, Shadyro OI.Prikl Biokhim Mikrobiol. 2012 May-Jun;48(3):282-8.PMID: 22834298 Russian.

The inhibition efficiency (antioxidant activity) of hydroxy derivatives of coumarin, such as esculetin, dicumarol, and fraxetin, was studied in the methemalbumin-H2O2-tetramethylbenzidine (TMB) pseudoperoxidase system at 20 degrees C in a buffered physiological solution (p …

Intracellular accumulation of bilirubin as a defense mechanism against increased oxidative stress. Zelenka J, Muchova L, Zelenkova M, Vanova K, Vreman HJ, Wong RJ, Vitek L.Biochimie. 2012 Aug;94(8):1821-7. doi: 10.1016/j.biochi.2012.04.026. Epub 2012 May 10.PMID: 22580386

Evidence of mutualism between two periodontal pathogens: co-operative haem acquisition by the HmuY haemophore of Porphyromonas gingivalis and the cysteine protease interpain A (InpA) of Prevotella intermedia. Byrne DP, Potempa J, Olczak T, Smalley JW.Mol Oral Microbiol. 2013 Jun;28(3):219-29. doi: 10.1111/omi.12018. Epub 2013 Jan 22.PMID: 23336115 Free PMC article.

The haem-binding lipoprotein HmuY haemophore and the gingipain proteases of P. gingivalis form a unique synthrophic system responsible for capture of haem from haemoglobin and methaemalbumin. In this system, methaemoglobin is formed from oxyhaemoglobin by the activities of … 

Tyrosine residues play an important role in heme detoxification by serum albumin.Huang Y, Shuai Y, Li H, Gao Z.Biochim Biophys Acta. 2014 Mar;1840(3):970-6. doi: 10.1016/j.bbagen.2013.11.011. Epub 2013 Nov 16.PMID: 24252277

METHODS: SDS-PAGE and Western blot were used to determine the efficiency of methemalbumin on catalyzing protein carbonylation and nitration. HPLC was used to test the formation of heme to protein cross-linked methemalbumin. …The heme to protein cross-linked form o …

Just Another Hemolysed Sample? Hamilton PK, Aldworth GE, Smye M, McDonnell M, Connolly GM.Clin Chem. 2015 Dec;61(12):1549-50. doi: 10.1373/clinchem.2015.242792.PMID: 26614230 No abstract available.

Heme Oxygenase Activity and Heme Binding in a Neonatal Mouse Model. Kourula S, Ang J, Zhao H, Kalish F, Vandenabeele P, Sylvester KG, Wong RJ, Stevenson DK.Neonatology. 2017;112(4):376-383. doi: 10.1159/000479493. Epub 2017 Sep 20.PMID: 28926834

METHODS: For in vitro studies, NIH3T3 HO-1-luc cells were incubated with 10, 30, or 60 M FH or methemalbumin (MHA). HO-1 promoter activity was assessed 3, 6, and 24 h after treatment. …

Application of HBOCs electrophoretic method to detect a new blood substitute derived from the giant extracellular haemoglobin of lugworm. Marchand A, Crepin N, Roulland I, Semence F, Domergue V, Zal F, Polard V, Coquerel A.Drug Test Anal. 2017 Nov;9(11-12):1762-1767. doi: 10.1002/dta.2127. Epub 2017 Jan 13.PMID: 27787946

An HC-derived product was identified in human plasma up to 72 h after in vitro incubation at 37 C. HC degradation also induced methemalbumin formation. After injecting HC at the effective dose of 200 mg/kg into mice, the HC-derived product was detected only for a few hours …

Acute haemolysis, DIC and renal failure after transfusion of uncross-matched blood during trauma resuscitation: illustrative case and literature review. Fiorellino J, Elahie AL, Warkentin TE.Transfus Med. 2018 Aug;28(4):319-325. doi: 10.1111/tme.12513. Epub 2018 Feb 19.PMID: 29460456 Review.

RESULTS: Acute HTR was shown by haemoglobinuria, free-plasma haemoglobin and methemalbumin, with anti-K and anti-Fy(a) eluted from recipient red cells; acute DIC featured severe hypofibrinogenemia, thrombocytopenia, elevated fibrin D-dimer and multiple bilateral renal infa …

Human Plasma and Recombinant Hemopexins: Heme Binding Revisited. Karnaukhova E, Owczarek C, Schmidt P, Schaer DJ, Buehler PW.Int J Mol Sci. 2021 Jan 26;22(3):1199. doi: 10.3390/ijms22031199.PMID: 33530421 Free PMC article.

Spectrophotometric evaluation of hemolysis in plasma by quantification of free oxyhemoglobin, methemoglobin, and methemalbumin in presence of bilirubin. Heckl C, Lang A, Rühm A, Sroka R, Duffield T, Vogeser M, Paal M.J Biophotonics. 2021 May;14(5):e202000461. doi: 10.1002/jbio.202000461. Epub 2021 Feb 13.PMID: 33527705

Severe intravascular hemolysis leads to the simultaneous presence of free heme pigments (oxyhemoglobin, methemoglobin, and methemalbumin) and bilirubin in human plasma. Standard spectrophotometric methods used to assess in vivo hemolysis inadequately address this complex a …

Abdominal Pain and Cola-Colored Serum. Tiel Groenestege WM, Wijnen EJ, Janssen MJW.Clin Chem. 2023 Feb 1;69(2):207-208. doi: 10.1093/clinchem/hvac193.PMID: 36724483 No abstract available.

Methaemalbumin: a diagnostic surrogate for methaemoglobinaemia and treatment with red cell exchange in a patient with thalassaemia. Prus-Czarnecka Z, Fuenzy AI, Noga T, Lau VI.BMJ Case Rep. 2023 Mar 7;16(3):e252885. doi: 10.1136/bcr-2022-252885.PMID: 36882261 Free PMC article.

However, the patient’s co-oximetry results were suppressed by the blood gas analyser and delayed a definitive diagnosis. A methaemalbumin screen was sent instead, which was positive at 65 mg/L (reference interval: <3 mg/L). …This resulted in rapid improvement without …