Thiocyanate Metal Binding and metal-binding (PubMed Article Search)
METAL-BINDING ARTICLES
Rocco D, Prescimone A, Constable EC, Housecroft CE.Molecules. 2020 Apr 4;25(7):1663. doi: 10.3390/molecules25071663.PMID: 32260325 Free PMC article.
An increase in the n-alkyloxy chain length has two consequences: there is a change in the conformation of the 3,2′:6′,3″-tpy metal–binding domain, and the (4,4) net comprises identical rhombuses. Similarities and differences between the assemblies with ligands 2-6 a …2Cite
Klein YM, Prescimone A, Karpacheva M, Constable EC, Housecroft CE.Polymers (Basel). 2018 Dec 11;10(12):1369. doi: 10.3390/polym10121369.PMID: 30961295 Free PMC article.
The syntheses and characterizations of three new ligands containing two 4,2′:6′,4″-tpy or two 3,2′:6′,3″-tpy metal–binding domains are reported. The ligands possess different alkyloxy functionalities attached to the central phenylene spacer: n-pentyloxy in 3, 4-phen …3Cite
Gardonio D, Siemann S.Biochem Biophys Res Commun. 2009 Mar 27;381(1):107-11. doi: 10.1016/j.bbrc.2009.02.021. Epub 2009 Feb 12.PMID: 193516044Cite
Thiocyanate hydrolase is a cobalt-containing metalloenzyme with a cysteine-sulfinic acid ligand.
Katayama Y, Hashimoto K, Nakayama H, Mino H, Nojiri M, Ono TA, Nyunoya H, Yohda M, Takio K, Odaka M.J Am Chem Soc. 2006 Jan 25;128(3):728-9. doi: 10.1021/ja057010q.PMID: 16417356
Thiocyanate hydrolase (SCNase) purified from Thiobacillus thioparus THI115 hydrolyzes thiocyanate to carbonyl sulfide and ammonia. …Mass spectrometry for the peptide fragment containing the metal–binding motif of the SCNase gamma subunit indicated th …5Cite
Structural transition in the metal-free hexamer of protein-engineered [B13 Gln]insulin.
Wollmer A, Rannefeld B, Stahl J, Melberg SG.Biol Chem Hoppe Seyler. 1989 Sep;370(9):1045-53. doi: 10.1515/bchm3.1989.370.2.1045.PMID: 2692616
Combination of transformed 2Zn[B13 Gln]insulin and metal-free native insulin in the absence of additives results in a redistribution of the zinc ions in favour of native insulin which is an outcome of the dynamic equilibrium and also demonstrates an influence of B13 charge on …6Cite
Miki M, dos Remedios CG.J Biochem. 1988 Aug;104(2):232-5. doi: 10.1093/oxfordjournals.jbchem.a122448.PMID: 3141394 Free article.
The resonance energy transfer between fluorescein-5-isothiocyanate (FITC) attached to Lys-61 and Co2+ bound to the high-affinity metal binding site was measured. The distance between FITC and Co2+ on the actin molecule was calculated to be either 1.9 nm, using the a …
METAL BINDING ARTICLES
[Mechanism of Thiocyanate Dehydrogenase Functioning Based on Structural Data].
Polyakov KM, Gavryushov S.Mol Biol (Mosk). 2025 Jan-Feb;59(1):141-153.PMID: 40542637 Russian.
Thiocyanate dehydrogenase is enzyme catalyzing transformation of a thiocyanate ion into a cyanate ion with outcome of two electrons, two protons and a neutral atom of sulphur. …The complex with thiourea allows us to model binding of thiocyanate ion t …2Cite
Altın S, Işık M, Alp C, Dikici E, Köksal E, Kırboğa KK, Rudrapal M, Rakshit G, Beydemir Ş, Khan J.Front Chem. 2025 Feb 19;13:1541250. doi: 10.3389/fchem.2025.1541250. eCollection 2025.PMID: 40046016 Free PMC article.
Antioxidant activity was assessed through ferric thiocyanate, DPPH, ABTS, and FRAP assays. Enzyme inhibition assays targeted AChE, BChE, and alpha-GLY, with IC50 values from dose-response curves. In silico analyses were conducted using molecular docking techniques to predi …3Cite
Xie YJ, Li TM, Shang ZT, Lu WT, Yu F.Dalton Trans. 2024 Aug 6;53(31):12985-12994. doi: 10.1039/d4dt01250j.PMID: 39027930
The design and development of new large-capacity and selective materials for extracting rare precious metals via electronic waste is practically essential. In this paper, a new efficient UiO-66-NCS has been obtained as a consequence of the modification of the classical Zr- …4Cite
Heteronuclear Complexes of Hg(II) and Zn(II) with Sodium Monensinate as a Ligand.
Pantcheva I, Petkov N, Encheva E, Kolev S, Simova S, Tsanev A, Dorkov P, Ugrinov A.Molecules. 2024 Jun 29;29(13):3106. doi: 10.3390/molecules29133106.PMID: 38999058 Free PMC article.
The commercial veterinary antibiotic sodium monensinate (MonNa) binds mercury(II) or zinc(II) cations as thiocyanate [Hg(MonNa)(2)(SCN)(2)] (1) or isothiocyanate [Zn(MonNa)(2)(NCS)(2)] (2) neutral coordination compounds. …Sodium cations remain in the hydrophilic c …5Cite
Jevtovic V, Alhar MSO, Milenković D, Marković Z, Dimitrić Marković J, Dimić D.Int J Mol Sci. 2023 Sep 29;24(19):14745. doi: 10.3390/ijms241914745.PMID: 37834192 Free PMC article.
The thermodynamic parameters of the binding process were determined. The binding to DNA, as one of the main pathways in the cell death cycle, was analyzed by molecular docking. …The most active compound was Cu-PLAG due to the presence of PLAG and two thiocyanat …6Cite
Hashemi Haeri H, Schneegans N, Eisenschmidt-Bönn D, Brandt W, Wittstock U, Hinderberger D.Biol Chem. 2023 Aug 17;405(2):105-118. doi: 10.1515/hsz-2023-0187. Print 2024 Feb 26.PMID: 37586381
Upon tissue damage, their myrosinase-catalyzed hydrolysis yields aglucones that rearrange to toxic isothiocyanates. Specifier proteins such as thiocyanate-forming protein from Thlaspi arvense (TaTFP) are non-heme iron proteins, which capture the aglucone to form alternativ …7Cite
Elucidating the concentration-dependent effects of thiocyanate binding to carbonic anhydrase.
Silva JM, Cerofolini L, Carvalho AL, Ravera E, Fragai M, Parigi G, Macedo AL, Geraldes CFGC, Luchinat C.J Inorg Biochem. 2023 Jul;244:112222. doi: 10.1016/j.jinorgbio.2023.112222. Epub 2023 Apr 11.PMID: 37068394 Free article.
Many proteins naturally carry metal centers, with a large share of them being in the active sites of several enzymes. …In this manuscript we investigate the effects of sodium thiocyanate on the chemical environment of the metal ion of the human carbonic anh …8Cite
How to identify a smoker: a salient crystallographic approach to detect thiocyanate content.
Iman K, Ahamad MN, Monika, Ansari A, Saleh HAM, Khan MS, Ahmad M, Haque RA, Shahid M.RSC Adv. 2021 May 7;11(28):16881-16891. doi: 10.1039/d1ra01749g. eCollection 2021 May 6.PMID: 35479719 Free PMC article.
In this study, a series of Co(7) clusters incorporating various flexible polyhydroxyamine ligands are explored, with the first report of thiocyanate recognition triggered by crystal formation using a Co(7) crystal (1). For this, we have fortunately synthesized three new mi …9Cite
Aspelin V, Lidskog A, Solano Arribas C, Hervø-Hansen S, Stenqvist B, Chudoba R, Wärnmark K, Lund M.J Am Chem Soc. 2022 Feb 23;144(7):2921-2932. doi: 10.1021/jacs.1c08507. Epub 2022 Feb 10.PMID: 35142499 Free PMC article.
A counterintuitive result was observed: the enthalpy change associated with the binding of the second ion is more negative than that of the first (deltaH(bind,2)() < deltaH(bind,1)()). …While the observed trend is independent of the counterion, the origin of the signi…10Cite
Jin G, Liu T, Li Y, Zhou J, Zhang D, Pang P, Ye Z, Xing Z, Xing G, Chen J, Ma D.Nanoscale. 2022 Jan 20;14(3):919-929. doi: 10.1039/d1nr06549a.PMID: 34988562
Quasi-2D metal halide perovskites are promising candidates for light-emitting applications owing to their large exciton binding energy and strong quantum confinement effect. …Butylammonium bromide (BABr) and potassium thiocyanate (KSCN) are employed to syne …11Cite
Zhou D, Zhang M, Ma Y, Mukherjee S, Liu J, Bian H.J Phys Chem B. 2021 Nov 25;125(46):12797-12805. doi: 10.1021/acs.jpcb.1c07902. Epub 2021 Nov 11.PMID: 34761933
It is usually believed that the binding affinity and selectivity of an alkali metal ion with crown ether are defined by the size matching model. …The results should help the researchers to unravel the specific host-guest interactions and further reveal the origina …12Cite
Ariffin EY, Zakariah EI, Ruslin F, Kassim M, Yamin BM, Heng LY, Hasbullah SA.Sci Rep. 2021 Apr 12;11(1):7883. doi: 10.1038/s41598-021-86939-z.PMID: 33846405 Free PMC article.
Novel hexaferrocenium tri[hexa(isothiocyanato)iron(III)]trihydroxonium (HexaFc) complex was the product from the reaction of ferrocene, maleic acid and ammonium thiocyanate and was confirmed by elemental analysis CHNS, FTIR and single crystal X-ray crystallography. …The …13Cite
Thermodiffusion of aqueous solutions of various potassium salts.
Mohanakumar S, Luettmer-Strathmann J, Wiegand S.J Chem Phys. 2021 Feb 28;154(8):084506. doi: 10.1063/5.0038039.PMID: 33639776
Thermophoresis or thermodiffusion has become an important tool to monitor protein-ligand binding as it is very sensitive to the nature of solute-water interactions. However, the microscopic mechanisms underlying thermodiffusion in protein systems are poorly understood at t …14Cite
Bai XR, Zhang L, Ren JQ, Shen AG, Hu JM.Anal Methods. 2021 Mar 4;13(8):1049-1057. doi: 10.1039/d0ay02221g.PMID: 33565531
Initially, we investigated the clustering and optical properties of noble metal sol nanoparticles (NPs) due to the competitive interaction of thiocyanate ions (SCN-) and cetyltrimethylammonium bromide (CTAB) under weak acidic conditions, and found that different dim …15Cite
Kang G, Jing Y, Liu W, Zhang C, Lu L, Chen C, Lu Y.Analyst. 2021 Mar 7;146(5):1650-1655. doi: 10.1039/d1an00002k. Epub 2021 Feb 1.PMID: 33522553
In this work, a novel convenient colorimetric method for sensitive detection of thiocyanate (SCN(-)) has been developed based on its suppression of the oxidase-like activity of palladium square nanoplates on reduced graphene oxide (Pd SP@rGO). SCN(-) can be adsorbed onto t …16Cite
Singh PK, Pandey S, Rani C, Ahmad N, Viswanathan V, Sharma P, Kaur P, Sharma S, Singh TP.J Biol Inorg Chem. 2021 Feb;26(1):149-159. doi: 10.1007/s00775-020-01844-6. Epub 2021 Jan 11.PMID: 33427997
Lactoperoxidase, a heme-containing glycoprotein, catalyzes the oxidation of thiocyanate by hydrogen peroxide into hypothiocyanite which acts as an antibacterial agent. The prosthetic heme moiety is attached to the protein through two ester linkages via Glu258 and Asp108. I …17Cite
Fentanyl Detection Using Eosin Y Paper Assays.
Canfield JR, Agarwal S, Fortener SK, Sprague JE.J Forensic Sci. 2020 Sep;65(5):1432-1442. doi: 10.1111/1556-4029.14437. Epub 2020 Apr 29.PMID: 32347988
In the present study, a variety of drugs of abuse and fentanyl analogues were tested to determine which drugs will bind to eosin Y, which functional groups are capable of binding and eliciting a color change, and a mechanism for eosin Y binding to fentanyl. …Throu …18Cite
Rocco D, Prescimone A, Constable EC, Housecroft CE.Molecules. 2020 Apr 4;25(7):1663. doi: 10.3390/molecules25071663.PMID: 32260325 Free PMC article.
An increase in the n-alkyloxy chain length has two consequences: there is a change in the conformation of the 3,2′:6′,3″-tpy metal–binding domain, and the (4,4) net comprises identical rhombuses. Similarities and differences between the assemblies with ligands 2-6 a …19Cite
Interaction of iron ions with melanin.
Żądło AC, Sarna T.Acta Biochim Pol. 2019 Dec 11;66(4):459-462. doi: 10.18388/abp.2019_2889.PMID: 31826048 Free article.
One of the antioxidant roles of melanin is binding redox-active transition metal ions. The aim of this study was to examine the redox reactions accompanying iron binding by melanin. Two kinds of synthetic eumelanin were mixed with iron (II) and iron (III) in …20Cite
Ascenzi P, De Simone G, Ciaccio C, Coletta M.J Inorg Biochem. 2020 Jan;202:110814. doi: 10.1016/j.jinorgbio.2019.110814. Epub 2019 Sep 2.PMID: 31733428
However, azide, thiocyanate and imidazole binding to Hb(III), Hp1-1:Hb(III) and Hp2-2:Hb(III) is characterized by a simple equilibrium, reflecting the compensation of kinetic parameters. The fast and the slow step of azide, thiocyanate and imidazole bindin …21Cite
Application of transition metal ions in a study of photoinduced modifications of melanin.
Żądło AC.Acta Biochim Pol. 2019 May 16;66(2):237-241. doi: 10.18388/abp.2018_2802.PMID: 31095651 Free article.
A synthetic model of eumelanin was treated by intense violet light. The interaction of melanin with metal ions was examined by electron paramagnetic resonance (EPR) spectroscopy and a thiocyanate assay. Weak photodegradation of melanin was shown to increase exposure …22Cite
Klein YM, Prescimone A, Karpacheva M, Constable EC, Housecroft CE.Polymers (Basel). 2018 Dec 11;10(12):1369. doi: 10.3390/polym10121369.PMID: 30961295 Free PMC article.
The syntheses and characterizations of three new ligands containing two 4,2′:6′,4″-tpy or two 3,2′:6′,3″-tpy metal–binding domains are reported. The ligands possess different alkyloxy functionalities attached to the central phenylene spacer: n-pentyloxy in 3, 4-phen …23Cite
Thiocyanate-induced labilization of schwertmannite: Impacts and mechanisms.
Fan C, Guo C, Zhang J, Ding C, Li X, Reinfelder JR, Lu G, Shi Z, Dang Z.J Environ Sci (China). 2019 Jun;80:218-228. doi: 10.1016/j.jes.2018.12.015. Epub 2018 Dec 29.PMID: 30952339
Understanding the mechanisms that induce schwertmannite labilization and affect its capacity to remove heavy metals are of great environmental and geochemical significance. Thiocyanate (SCN) is a hazardous pseudohalide that is also normally found in AMD. However, li …24Cite
Chemical and Clinical Aspects of Metal-Containing Antidotes for Poisoning by Cyanide.
Suman SG, Gretarsdottir JM.Met Ions Life Sci. 2019 Jan 14;19:/books/9783110527872/9783110527872-020/9783110527872-020.xml. doi: 10.1515/9783110527872-020.PMID: 30855115 Free article. Review.
Only a handful of antidotes for treatment of cyanide poisoning are known; they are primarily inorganic compounds and metal complexes which are intended to intercept cyanide before it inhibits cellular respiration. The inorganic compounds manipulate hemoglobin, forming meth …25Cite
Iron is a centrally bound cofactor of specifier proteins involved in glucosinolate breakdown.
Backenköhler A, Eisenschmidt D, Schneegans N, Strieker M, Brandt W, Wittstock U.PLoS One. 2018 Nov 5;13(11):e0205755. doi: 10.1371/journal.pone.0205755. eCollection 2018.PMID: 30395611 Free PMC article.
Iron binding and activity depend on E266, D270, and H274 suggesting a direct interaction of Fe2+ with these residues. …This may explain why AtNSP3 does not support epithionitrile or thiocyanate formation, which likely requires exact positioning of the aglucone thi …26Cite
da Silva VHM, Quattrociocchi DGS, Stoyanov SR, Carneiro JWM, da Costa LM, Ferreira GB.J Mol Model. 2018 Jan 8;24(1):39. doi: 10.1007/s00894-017-3571-x.PMID: 29313121
The electronic effects (resonance and induction) of neighboring groups of the interacting atom modulate the strength of metal-ligand binding. For almost all the O-donor ligands the electrostatic component has the same magnitude as the covalent term, while for the N- …27Cite
Zhou A, Crossland PM, Draksharapu A, Jasniewski AJ, Kleespies ST, Que L Jr.J Biol Inorg Chem. 2018 Jan;23(1):155-165. doi: 10.1007/s00775-017-1517-5. Epub 2017 Dec 7.PMID: 29218640 Free PMC article.
Nonheme oxoiron(IV) complexes can serve as synthons for generating heterobimetallic oxo-bridged dimetal complexes by reaction with divalent metal complexes. The formation of Fe(III)-O-Cr(III) and Fe(III)-O-Mn(III) complexes is described herein. …The positions of these fe …28Cite
Wojtyła S, Macyk W, Baran T.Photochem Photobiol Sci. 2017 Jul 1;16(7):1079-1087. doi: 10.1039/c6pp00465b. Epub 2017 May 17.PMID: 28513736
The photosensitization mechanism encompasses a metal to metal charge transfer, Cu(I) Cu(II). The applied photosensitizer binds to CuI through thiocyanate ligands resulting in the formation of an active Cu(II)/Cu(I) hybrid photocatalyst ([Cu(II)(SCN)(2) …29Cite
Datta D, J Swamy M.J Photochem Photobiol B. 2017 May;170:108-117. doi: 10.1016/j.jphotobiol.2017.03.026. Epub 2017 Apr 1.PMID: 28414980
MLGL (Mulberry Latex Galactose-specific Lectin) is an alpha-d-galactose binding lectin isolated from the latex of mulberry (Morus indica) tree and contains two tryptophan residues in each of its subunits. The fluorescence emission maximum of native MLGL seen at 326nm shift …30Cite
Self-tuning interfacial architecture for Estradiol detection by surface plasmon resonance biosensor.
Boltovets P, Shinkaruk S, Vellutini L, Snopok B.Biosens Bioelectron. 2017 Apr 15;90:91-95. doi: 10.1016/j.bios.2016.11.017. Epub 2016 Nov 12.PMID: 27886605
The sensing interfacial architecture is based on the BSA-E2 conjugate within the BSA matrix immobilized on the “charged” (as a result of guanidine thiocyanate treatment) gold surface at pH 5.0. Kinetic analysis for different E2 concentrations shows that using parameter bet …31Cite
Jupp AR, Geeson MB, McGrady JE, Goicoechea JM.Eur J Inorg Chem. 2016 Feb;2016(5):639-648. doi: 10.1002/ejic.201501075. Epub 2015 Nov 4.PMID: 27134553 Free PMC article.
Our studies reveal that, in the coordination sphere of tungsten(0), the PCO(-) anion preferentially binds through the phosphorus atom in a strongly bent fashion, while NCO(-) and NCS(-) coordinate linearly through the nitrogen atom. …It was possible to establish that, as …32Cite
Komarnicka UK, Starosta R, Płotek M, de Almeida RF, Jeżowska-Bojczuk M, Kyzioł A.Dalton Trans. 2016 Mar 28;45(12):5052-63. doi: 10.1039/c5dt04011f.PMID: 26674970
In this paper we present a first insight into the cytotoxic action mode of copper(I) iodide or copper(I) thiocyanate complexes with a phosphine derivative of sparfloxacin (a 3rd generation fluoroquinolone antibiotic agent) and 2,9-dimethyl-1,10-phenanthroline or 2,2′-biqui …33Cite
Auer M, Nicolussi A, Schütz G, Furtmüller PG, Obinger C.J Biol Chem. 2014 Nov 7;289(45):31480-91. doi: 10.1074/jbc.M114.595157. Epub 2014 Sep 22.PMID: 25246525 Free PMC article.
Moreover, apparent bimolecular rate constants of the reaction of compound I with iodide, thiocyanate, bromide, and tyrosine increased significantly and became similar to LPO. Kinetic data are discussed and compared with known structure-function relationships of the mammali …34Cite
Neu HM, Yang T, Baglia RA, Yosca TH, Green MT, Quesne MG, de Visser SP, Goldberg DP.J Am Chem Soc. 2014 Oct 1;136(39):13845-52. doi: 10.1021/ja507177h. Epub 2014 Sep 19.PMID: 25238495 Free PMC article.
A wide range of OAT reactivity is seen for the different axial ligands, with the following trend determined from a comparison of their second-order rate constants for sulfoxidation: five-coordinate thiocyanate nitrate < cyanate < azide < fluoride cyanide. This tre…35Cite
Hazari A, Das LK, Bauzá A, Frontera A, Ghosh A.Dalton Trans. 2014 Jun 7;43(21):8007-15. doi: 10.1039/c3dt53318b. Epub 2014 Apr 9.PMID: 24715108
Theoretical calculations on the energetic difference between the two possible coordination modes of the thiocyanate anion to the Cd atom reveal that N-coordination is preferred over S-coordination in agreement with the much greater abundance of the reported N-bonded struct …36Cite
Chiniforoshan H, Tabrizi L, Hadizade M, Sabzalian MR, Chermahini AN, Rezapour M.Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15;128:183-90. doi: 10.1016/j.saa.2014.02.135. Epub 2014 Mar 12.PMID: 24667423
The binding modes of the ligands in complexes were established by means of molecular modeling of the complexes, and calculation of their IR, NMR and absorption spectra at DFT (TDDFT)/B3LYP level were studied. The experimental and calculated data verified monodentate bin …37Cite
Hao Y, Xiong D, Wang L, Chen W, Zhou B, Liu YN.Talanta. 2013 Oct 15;115:253-7. doi: 10.1016/j.talanta.2013.04.076. Epub 2013 May 3.PMID: 24054588
A novel reversible colorimetric sensor, which based on a competitive ligation of Hg(2+) by thiols, cysteine (Cys) or glutathione (GSH), and thiocyanate (SCN) on the N3 dye (bis(4,4′-dicarboxy-2,2′-bipyridine) dithiocyanato ruthenium (II)), was developed for the detection o …38Cite
Yamanaka Y, Arakawa T, Watanabe T, Namima S, Sato M, Hori S, Ohtaki A, Noguchi K, Katayama Y, Yohda M, Odaka M.J Biosci Bioeng. 2013 Jul;116(1):22-7. doi: 10.1016/j.jbiosc.2013.01.013. Epub 2013 Mar 1.PMID: 23453853
Thiocyanate hydrolase (SCNase) of Thiobacillus thioparus THI115 is a cobalt (Co)-containing enzyme that catalyzes the hydrolysis of thiocyanate (SCN-), a major component of wastewater from coke oven factories, to carbonyl sulfide and ammonia. …Based on the differe …39Cite
Li X, Reynal A, Barnes P, Humphry-Baker R, Zakeeruddin SM, De Angelis F, O’Regan BC.Phys Chem Chem Phys. 2012 Nov 28;14(44):15421-8. doi: 10.1039/c2cp43347h. Epub 2012 Oct 15.PMID: 23070136
From FTIR results and molecular modelling we show the iodine binds to the thiocyanate group in all these dyes. For the AR24 dye we present evidence that iodine also binds to the amine moiety on this dye. …By comparison of recombination data on full cells, w …40Cite
Cook NL, Pattison DI, Davies MJ.Free Radic Biol Med. 2012 Dec 1;53(11):2072-80. doi: 10.1016/j.freeradbiomed.2012.09.033. Epub 2012 Sep 29.PMID: 23032100
Zinc is an abundant cellular transition metal ion, which binds avidly to protein cysteine (Cys) and histidine (His) residues to form zinc-Cys/His clusters; these play a key role in the function of many proteins (e.g., DNA binding and repair enzymes, transcrip …41Cite
Metal-organic framework supported ionic liquid membranes for CO2 capture: anion effects.
Gupta KM, Chen Y, Hu Z, Jiang J.Phys Chem Chem Phys. 2012 Apr 28;14(16):5785-94. doi: 10.1039/c2cp23972h. Epub 2012 Mar 20.PMID: 22433933
The small anions [PF(6)](-), [BF(4)](-), and [SCN](-) prefer to locate near to the metal-cluster, particularly the quasi-spherical [PF(6)](-) and [BF(4)](-). In contrast, the bulky and chain-like [BMIM](+) and [Tf(2)N](-) reside near the phenyl ring. …This hierarchy is p …42Cite
Souza CE, Maitra D, Saed GM, Diamond MP, Moura AA, Pennathur S, Abu-Soud HM.PLoS One. 2011;6(11):e27641. doi: 10.1371/journal.pone.0027641. Epub 2011 Nov 22.PMID: 22132121 Free PMC article.
Lactoperoxidase (LPO) is the major consumer of hydrogen peroxide (H(2)O(2)) in the airways through its ability to oxidize thiocyanate (SCN(-)) to produce hypothiocyanous acid, an antimicrobial agent. In nasal inflammatory diseases, such as cystic fibrosis, both LPO and mye …43Cite
Lim J, Kwon YS, Park SH, Song IY, Choi J, Park T.Langmuir. 2011 Dec 6;27(23):14647-53. doi: 10.1021/la2026329. Epub 2011 Oct 26.PMID: 21988282
Slowing of adsorption, both by thermal means and through SA-mediated processes, effectively controlled the binding mode of N719 on the surface of TiO(2). The resulting sensitized electrodes displayed enhanced device longevity and improved generation of photoinduced electro …44Cite
Fafarman AT, Koh WK, Diroll BT, Kim DK, Ko DK, Oh SJ, Ye X, Doan-Nguyen V, Crump MR, Reifsnyder DC, Murray CB, Kagan CR.J Am Chem Soc. 2011 Oct 5;133(39):15753-61. doi: 10.1021/ja206303g. Epub 2011 Sep 9.PMID: 21848336
The short, air-stable, environmentally benign thiocyanate ligand electrostatically stabilizes a variety of semiconductor and metallic NCs in polar solvents, allowing solution-based deposition of NCs into thin-film NC solids. …The spectroscopic properties of this l …45Cite
Chen FJ, Xu M, Xi PX, Liu HY, Zeng ZZ.Spectrochim Acta A Mol Biomol Spectrosc. 2011 Oct 15;81(1):21-7. doi: 10.1016/j.saa.2011.05.020. Epub 2011 Jun 12.PMID: 21723777
Two novel copper(II) thiocyanate complexes with 4-(N,N-dimethylamino) pyridine and N,N-dimethylformamide (1) and with 4-(N,N-dimethylamino) pyridine (2) have been synthesized and characterized. …46Cite
Han Y, Lupitskyy R, Chou TM, Stafford CM, Du H, Sukhishvili S.Anal Chem. 2011 Aug 1;83(15):5873-80. doi: 10.1021/ac2005839. Epub 2011 Jul 13.PMID: 21644591
Finally, we also found UV irradiation and ozone had a synergistic effect on silver oxidation and thus a detrimental effect on SERS enhancement of Ag NPs and that such oxidation effects were analyte-dependent, as a result of inherent differences in chemical enhancements and molecu …47Cite
Shin JW, Rowthu SR, Hyun MY, Song YJ, Kim C, Kim BG, Min KS.Dalton Trans. 2011 Jun 7;40(21):5762-73. doi: 10.1039/c1dt10028a. Epub 2011 Apr 27.PMID: 21523305
In complex 1 the Mn(II) is bonded to one bpaeOH and two thiocyanate ions, while in complex 2 it is coordinated to a deprotonated bpaeO(-) and two azide ions. The oxidation states of manganese ions are 2+ for 1 and 3+ for 2, respectively, indicating that the different oxida …48Cite
Shearer J, Callan PE, Amie J.Inorg Chem. 2010 Oct 4;49(19):9064-77. doi: 10.1021/ic101765h.PMID: 20831172 Free PMC article.
These enzymes are redox inactive and perform hydrolytic reactions; SCNase hydrolyzes thiocyanate anions while NHase converts nitriles into amides. Herein an active CoNHase metallopeptide mimic, [Co(III)NHase-m1] (NHase-m1 = AcNH-CCDLP-CGVYD-PA-COOH), that contains Co(III) …49Cite
The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008.
Tsien RY.J Vis Exp. 2010 Jan 13;(35):1575. doi: 10.3791/1575.PMID: 20072108 Free PMC article.
From growing silica gardens of colorful crystallized metal salts to attempting to synthesize aspirin, these early experiments fueled what would become Tsien’s lifelong interest in chemistry and colors. Tsien’s first official laboratory experience was an NSF-supported summe …50Cite
Pseudo-halide derivatives of titanocene: synthesis and cytotoxicity studies.
Claffey J, Deally A, Gleeson B, Hogan M, Méndez LM, Müller-Bunz H, Patil S, Wallis D, Tacke M.Metallomics. 2009 Nov;1(6):511-7. doi: 10.1039/b911753a. Epub 2009 Sep 2.PMID: 21305159
The well-known anticancer drug candidate bis-[(p-methoxybenzyl)cyclopentadienyl] titanium(IV) dichloride (Titanocene ) was reacted with sodium azide or potassium cyanate, thiocyanate or selenocyanate in order to give pseudo-halide analogues of Titanocene . and were charact …51Cite
Kinetics of cyanide binding as a probe of local stability/flexibility of cytochrome c.
Varhac R, Tomásková N, Fabián M, Sedlák E.Biophys Chem. 2009 Sep;144(1-2):21-6. doi: 10.1016/j.bpc.2009.06.001. Epub 2009 Jun 6.PMID: 19545938
Effect of anions of the Hofmeister series (thiocyanate, perchlorate, iodide, bromide, nitrate, chloride, sulfate, and phosphate) on local and global stability and flexibility of horse heart ferricytochrome c (cyt c) has been studied. …Further, our results demonstrate sen …52Cite
Wang F, Habtemariam A, van der Geer EP, Deeth RJ, Gould R, Parsons S, Sadler PJ.J Biol Inorg Chem. 2009 Sep;14(7):1065-76. doi: 10.1007/s00775-009-0549-x. Epub 2009 Jun 5.PMID: 19499253
Detailed kinetic studies suggest that complex 1 binds to guanosine 5′-monophosphate (GMP) through direct N7 substitution of the N-bound SCN ligand. …53Cite
Gardonio D, Siemann S.Biochem Biophys Res Commun. 2009 Mar 27;381(1):107-11. doi: 10.1016/j.bbrc.2009.02.021. Epub 2009 Feb 12.PMID: 19351604
A method involving the reversible chemical modification of an active site, zinc-binding cysteine residue (Cys221) for the specific removal of one of the two zinc ions in the metallo-beta-lactamase IMP-1 was explored. …54Cite
Singh AK, Singh N, Sharma S, Shin K, Takase M, Kaur P, Srinivasan A, Singh TP.Biophys J. 2009 Jan;96(2):646-54. doi: 10.1016/j.bpj.2008.09.019.PMID: 19167310 Free PMC article.
In the presence of hydrogen peroxide, it preferentially converts thiocyanate ion into a toxic hypothiocyanate ion. Samples of bovine lactoperoxidase containing thiocyanate (SCN(-)) and hypothiocyanate (OSCN(-)) ions were purified and crystallized. …A phosphorylate …55Cite
Darensbourg DJ, Frantz EB.Inorg Chem. 2008 Jun 2;47(11):4977-87. doi: 10.1021/ic800286p. Epub 2008 Apr 26.PMID: 18439001
The presence of the electron-withdrawing -CF3 substituents enhances the electrophilicity of the metal center in these (tfacacen)MnX (X=Cl, N3, NCO, NCS) derivatives when compared with their (acacen)MnX (acacen=N, N’-bis(acetylacetone)-1,2-ethylenediimine) analogs. …Indee …56Cite
Wollers S, Heidenreich T, Zarepour M, Zachmann D, Kraft C, Zhao Y, Mendel RR, Bittner F.J Biol Chem. 2008 Apr 11;283(15):9642-50. doi: 10.1074/jbc.M708549200. Epub 2008 Feb 7.PMID: 18258600 Free article.
Furthermore, cyanide-treated ABA3 C terminus was shown to release thiocyanate, indicating that the molybdenum cofactor bound to the C-terminal domain is present in the sulfurated form. …57Cite
Crystal structure of lactoperoxidase at 2.4 A resolution.
Singh AK, Singh N, Sharma S, Singh SB, Kaur P, Bhushan A, Srinivasan A, Singh TP.J Mol Biol. 2008 Feb 29;376(4):1060-75. doi: 10.1016/j.jmb.2007.12.012. Epub 2007 Dec 14.PMID: 18191143
Lactoperoxidase (LPO) is a member of the mammalian peroxidase superfamily. It catalyzes the oxidation of thiocyanate and halides. Freshly isolated and purified samples of caprine LPO were saturated with ammonium iodide and crystallized using 20% polyethylene glycol 3350 in …58Cite
Rangarajan ES, Asinas A, Proteau A, Munger C, Baardsnes J, Iannuzzi P, Matte A, Cygler M.J Bacteriol. 2008 Feb;190(4):1447-58. doi: 10.1128/JB.01610-07. Epub 2007 Dec 7.PMID: 18065529 Free PMC article.
[NiFe] hydrogenases are heterodimeric Fe-S proteins, with a large subunit containing the reaction center involving Fe and Ni metal ions and a small subunit containing one or more Fe-S clusters. Maturation of the [NiFe] hydrogenase involves assembly of nonproteinaceous liga …59Cite
Croucher PD, Klingele MH, Noble A, Brooker S.Dalton Trans. 2007 Sep 28;(36):4000-7. doi: 10.1039/b707905b. Epub 2007 Aug 2.PMID: 1782836060Cite
Wang QX, Jiao K, Liu FQ, Yuan XL, Sun W.J Biochem Biophys Methods. 2007 Apr 10;70(3):427-33. doi: 10.1016/j.jbbm.2006.09.011. Epub 2006 Oct 3.PMID: 17222457
Its DNA-binding properties were studied by electronic absorption spectral, viscositive and electrochemical measurements. …The slight differences of peak profiles and electrochemical parameters between free and DNA-bound Ni(NCS)(2)(Mim)(4) showed the formation of an elect …61Cite
Arakawa T, Kawano Y, Kataoka S, Katayama Y, Kamiya N, Yohda M, Odaka M.J Mol Biol. 2007 Mar 9;366(5):1497-509. doi: 10.1016/j.jmb.2006.12.011. Epub 2006 Dec 8.PMID: 17222425
Thiocyanate hydrolase (SCNase) of Thiobacillus thioparus THI115 is a cobalt(III)-containing enzyme catalyzing the degradation of thiocyanate to carbonyl sulfide and ammonia. …The size as well as the electrostatic properties of the substrate-binding pocket w …62Cite
Huus K, Havelund S, Olsen HB, van de Weert M, Frokjaer S.Pharm Res. 2006 Nov;23(11):2611-20. doi: 10.1007/s11095-006-9098-y. Epub 2006 Sep 13.PMID: 16969698
RESULTS: An excellent correlation between the thermal stabilization by ligand binding and the deamidation of Asn(B3) was observed. The correlation between thermal stability and the formation of covalent dimer and other insulin related products was less clear. …At low tem …63Cite
Dye-sensitized SnO2 electrodes with iodide and pseudohalide redox mediators.
Bergeron BV, Marton A, Oskam G, Meyer GJ.J Phys Chem B. 2005 Jan 20;109(2):937-43. doi: 10.1021/jp0461347.PMID: 16866462
Adsorption isotherms of the sensitizers Ru(deeb)(bpy)2(PF6)2, Ru(deeb)2(dpp)(PF6)2, and Ru(deeb2(bpz)(PF6)2, where deeb is 4,4′-diethylester-2,2′-bipyridine, dpp is 2,3-dipyridyl pyrazine, and bpz is bipyrazine, binding to the SnO2 surface were well described by the Langmu …64Cite
Karmakar S, Weinberg MS, Banik NL, Patel SJ, Ray SK.Neuroscience. 2006 Sep 1;141(3):1265-80. doi: 10.1016/j.neuroscience.2006.04.075. Epub 2006 Jun 12.PMID: 16765523
Two of the inhibitor-of-apoptosis proteins were downregulated because of an increase in ‘second mitochondrial activator of caspases/Direct inhibitor-of-apoptosis protein binding protein with low pI.’ Decrease in nuclear factor kappa B and increase in inhibitor of nuclear f …65Cite
Ligand binding and thermostability of different allosteric states of the insulin zinc-hexamer.
Huus K, Havelund S, Olsen HB, Sigurskjold BW, van de Weert M, Frokjaer S.Biochemistry. 2006 Mar 28;45(12):4014-24. doi: 10.1021/bi0524520.PMID: 16548529
The relative affinity of the four tested anionic ligands was found, by DSC, to be thiocyanate > or = 4-hydroxy-3-nitrobenzoate >> p-aminobenzoate >> chloride. The results correlate with other methods and demonstrate that DSC provides a general and useful met …66Cite
Thiocyanate hydrolase is a cobalt-containing metalloenzyme with a cysteine-sulfinic acid ligand.
Katayama Y, Hashimoto K, Nakayama H, Mino H, Nojiri M, Ono TA, Nyunoya H, Yohda M, Takio K, Odaka M.J Am Chem Soc. 2006 Jan 25;128(3):728-9. doi: 10.1021/ja057010q.PMID: 16417356
Thiocyanate hydrolase (SCNase) purified from Thiobacillus thioparus THI115 hydrolyzes thiocyanate to carbonyl sulfide and ammonia. …Mass spectrometry for the peptide fragment containing the metal–binding motif of the SCNase gamma subunit indicated th …67Cite
Henriques BJ, Saraiva LM, Gomes CM.J Biol Inorg Chem. 2006 Jan;11(1):73-81. doi: 10.1007/s00775-005-0055-8. Epub 2005 Dec 6.PMID: 16331403
Here we report studies on the conformational stability of a new type of rubredoxin from the hyperthermophile Methanocaldococcus jannaschii, having an atypical metal site geometry resulting from a modified iron-binding motif. Absorption and fluorescence spectroscopie …68Cite
Covarrubias AS, Bergfors T, Jones TA, Högbom M.J Biol Chem. 2006 Feb 24;281(8):4993-9. doi: 10.1074/jbc.M510756200. Epub 2005 Dec 1.PMID: 16321983 Free article.
Here we present the structure of the thiocyanate complexed protein in a different crystal form. The protein now forms distinct tetramers and shows large structural changes, including a carboxylate shift yielding the accessible active site. …69Cite
Badr IH, Meyerhoff ME.Anal Chem. 2005 Oct 15;77(20):6719-28. doi: 10.1021/ac050987t.PMID: 16223262 Free PMC article.
The presence of the pH indicator and the simultaneous fluoride/proton coextraction equilibrium chemistry is shown to greatly enhance the reversibility of fluoride binding to the Al(III) porphyrin. Optical response toward fluoride can be observed in the range of 0.1 microM- …70Cite
Thiocyanate modulates the catalytic activity of mammalian peroxidases.
Tahboub YR, Galijasevic S, Diamond MP, Abu-Soud HM.J Biol Chem. 2005 Jul 15;280(28):26129-36. doi: 10.1074/jbc.M503027200. Epub 2005 May 13.PMID: 15894800 Free article.
We investigated the potential role of the co-substrate, thiocyanate (SCN-), in modulating the catalytic activity of myeloperoxidase (MPO) and other members of the mammalian peroxidase superfamily (lactoperoxidase (LPO) and eosinophil peroxidase (EPO)). …Consistent with t …71Cite
Lead(II) thiocyanate complexes with bibracchial lariat ethers: an X-ray and DFT study.
Platas-Iglesias C, Esteban-Gómez D, Enríquez-Pérez T, Avecilla F, de Blas A, Rodríguez-Blas T.Inorg Chem. 2005 Apr 4;44(7):2224-33. doi: 10.1021/ic048768y.PMID: 15792457
The coordination sphere of lead(II) is completed by two thiocyanate groups that coordinate either through their nitrogen (1) or sulfur (2) atoms. …To rationalize these unexpected different conformations of the L2 and L4 receptors in compounds 1 and 2, as well as the diff …72Cite
Jeong JM, Hong MK, Kim YJ, Lee J, Kang JH, Lee DS, Chung JK, Lee MC.Nucl Med Commun. 2004 Dec;25(12):1211-7. doi: 10.1097/00006231-200412000-00010.PMID: 15640781
BACKGROUND AND AIM: Various mannose receptor-binding agents, for example 99mTc-diethylenetriaminepentaacetic acid (DTPA)-mannosyl-polymer, have been developed for sentinel lymph node (SLN) imaging. In order to simplify the synthesis and labelling procedure and to improve t …73Cite
Innocenti A, Zimmerman S, Ferry JG, Scozzafava A, Supuran CT.Bioorg Med Chem Lett. 2004 Sep 6;14(17):4563-7. doi: 10.1016/j.bmcl.2004.06.073.PMID: 15357993
Fluoride, chloride, and sulfate do not inhibit this enzyme appreciably, whereas the CA substrate bicarbonate, or other anions, such as bromide, nitrite, bisulfite, or sulfamate behave as weak inhibitors (Ki in the range of 40-45 mM). It is interesting to note that the metal …74Cite
Blokesch M, Paschos A, Bauer A, Reissmann S, Drapal N, Böck A.Eur J Biochem. 2004 Aug;271(16):3428-36. doi: 10.1111/j.1432-1033.2004.04280.x.PMID: 15291820 Free article.
It does not contain statistically significant amounts of metals or of cofactors absorbing in the UV and visible light range. The protein displays low intrinsic ATPase activity with ADP and phosphate as the products, the apparent K(m) being 25 micro m and the k(cat) 1.7 x 1 …75Cite
Sun YJ, Zhang LZ, Cheng P, Lin HK, Yan SP, Liao DZ, Jiang ZH, Shen PW.Biophys Chem. 2004 May 1;109(2):281-93. doi: 10.1016/j.bpc.2003.12.001.PMID: 1511094676Cite
New inhibitors for the neutral amino acid transporter ASCT2 reveal its Na+-dependent anion leak.
Grewer C, Grabsch E.J Physiol. 2004 Jun 15;557(Pt 3):747-59. doi: 10.1113/jphysiol.2004.062521. Epub 2004 Apr 23.PMID: 15107471 Free PMC article.
We found that the leak anion conductance was inhibited by the binding of the newly characterized inhibitors benzylserine and benzylcysteine to ASCT2. These inhibitors competitively prevent binding of transported substrates to ASCT2, suggesting that they bind to the …77Cite
Elliott SJ, Hoke KR, Heffron K, Palak M, Rothery RA, Weiner JH, Armstrong FA.Biochemistry. 2004 Jan 27;43(3):799-807. doi: 10.1021/bi035869j.PMID: 14730985
This leads to the hypothesis that nitrate binds more tightly to Mo(V) than Mo(IV), so that low levels of nitrate are more effectively reduced at a higher potential despite the lower driving force. However, an alternative interpretation, that nitrate binding is affec …78Cite
Anion selectivity by the sodium iodide symporter.
Van Sande J, Massart C, Beauwens R, Schoutens A, Costagliola S, Dumont JE, Wolff J.Endocrinology. 2003 Jan;144(1):247-52. doi: 10.1210/en.2002-220744.PMID: 1248835179Cite
Goussias C, Deligiannakis Y, Sanakis Y, Ioannidis N, Petrouleas V.Biochemistry. 2002 Dec 24;41(51):15212-23. doi: 10.1021/bi026223e.PMID: 12484759
In the present study, we report on an unusual new mode of transient binding of NO, which is favored in the light-reduced state (Q(A)(-)Fe(2+)Q(B)) of the complex. …(vii) The spectral shape and spin concentration of these signals is successfully reproduced, assuming a wea …80Cite
The first example of a nitrile hydratase model complex that reversibly binds nitriles.
Shearer J, Jackson HL, Schweitzer D, Rittenberg DK, Leavy TM, Kaminsky W, Scarrow RC, Kovacs JA.J Am Chem Soc. 2002 Sep 25;124(38):11417-28. doi: 10.1021/ja012555f.PMID: 12236756 Free PMC article.
Reported herein is a five-coordinate model compound ([Fe(III)(S(2)(Me2)N(3)(Et,Pr))](+)) containing Fe(III) in an environment resembling that of NHase, which reversibly binds a variety of nitriles, alcohols, amines, and thiocyanate. XAS shows that five-coordinate [F …81Cite
Wood CM, Matsuo AY, Gonzalez RJ, Wilson RW, Patrick ML, Val AL.J Exp Biol. 2002 Oct;205(Pt 19):3039-54. doi: 10.1242/jeb.205.19.3039.PMID: 12200407
Na(+), Cl(-) and Ca(2+) uptake were all strongly inhibited by acute exposure to pH 4.0, but efflux rates and Ca(2+) binding to the body surface did not change. Na(+) influx was inhibited by amiloride (10(-4) mol l(-1)) and by two of its analogs, phenamil (4 x 10(-5) mol l( …82Cite
Desmarais WT, Bienvenue DL, Bzymek KP, Holz RC, Petsko GA, Ringe D.Structure. 2002 Aug;10(8):1063-72. doi: 10.1016/s0969-2126(02)00810-9.PMID: 12176384 Free article.
The aminopeptidase from Aeromonas proteolytica (AAP) is a bridged bimetallic enzyme that removes the N-terminal amino acid from a peptide chain. To fully understand the metal roles in the reaction pathway of AAP we have solved the 1.20 A resolution crystal structure of nat …83Cite
Kojoh K, Fukuda E, Matsuzawa H, Wakagi T.J Inorg Biochem. 2002 Apr 10;89(1-2):69-73. doi: 10.1016/s0162-0134(01)00410-x.PMID: 11931965
The stability of the protein was examined by thermal degradation of the clusters. In the absence of guanidium thiocyanate, the T(m), defined as the mid-point temperature of the thermal transition from the native to the denatured state, for every mutant was above 100 degree …84Cite
Cornejo LS, López de Blanc S, Femopase F, Azcurra A, Calamari S, Battellino LJ, Dorronsoro de Cattoni ST.Acta Odontol Latinoam. 1998;11(1):15-25.PMID: 11885450 Clinical Trial.
The parameters evaluated were oral mucous membrane lesion index (MLI), CFU of Candida, saliva flow rate, protein-bound Fe (Fe-prot), Fe-prot binding capacity (Fe-prot cap), IgAs, peroxidase activity (PA), hypothiocyanite and thiocyanite. …85Cite
Piper AS, Greenwood IA, Large WA.J Physiol. 2002 Feb 15;539(Pt 1):119-31. doi: 10.1113/jphysiol.2001.013270.PMID: 11850506 Free PMC article.
These results suggest that NFA and DCDPC, but not DIDS, simultaneously enhance and block I(Cl(Ca)) by binding to an external site, probably close to the mouth of the chloride channel….86Cite
Raman signatures of ligand binding and allosteric conformation change in hexameric insulin.
Ferrari D, Diers JR, Bocian DF, Kaarsholm NC, Dunn MF.Biopolymers. 2001;62(5):249-60. doi: 10.1002/bip.1020.PMID: 11745120
These allosteric states are stabilized by the binding of ligands to the phenolic pockets and by the coordination of anions to the His B10 metal sites. Raman difference (RD) spectroscopy is utilized to examine the binding of phenolic ligands and the binding …87Cite
Shearer J, Kung IY, Lovell S, Kaminsky W, Kovacs JA.J Am Chem Soc. 2001 Jan 24;123(3):463-8. doi: 10.1021/ja002642s.PMID: 11456548 Free PMC article.
Complex 2 is a six-coordinate Co(III) complex containing cis-thiolates and imine nitrogens, and has properties similar to the cobalt center of Co NHase. Substrate binding to 1 is reversible and temperature-dependent, allowing for the determination of the thermodynamic para …88Cite
Decomposition of alkyl-substituted urea molecules at a hydroxide-bridged dinickel center.
Barrios AM, Lippard SJ.Inorg Chem. 2001 Mar 12;40(6):1250-5. doi: 10.1021/ic000933w.PMID: 11300826
After long reaction times, thiourea reacts similarly, producing ammonium thiocyanate. The other substrates are unreactive. These results indicate that the dinickel complex promotes the elimination of alkylamines from urea substrates to form cyanate but cannot effect the di …89Cite
Butler CS, Charnock JM, Garner CD, Thomson AJ, Ferguson SJ, Berks BC, Richardson DJ.Biochem J. 2000 Dec 15;352 Pt 3(Pt 3):859-64.PMID: 11104696 Free PMC article.
In the present study the effect of KSCN (potassium thiocyanate) as an inhibitor and Mo ligand has been investigated. Results are presented that show NAP is sensitive to SCN(-) (thiocyanate) inhibition, with SCN(-) acting as a competitive inhibitor of nitrate (K(i) a …90Cite
Shneine J, Voswinkel M, Federwisch M, Wollmer A.Biol Chem. 2000 Feb;381(2):127-33. doi: 10.1515/BC.2000.018.PMID: 10746744
The dependence of the transition on the nature of the ligands inducing it, such as inorganic anions or phenolic compounds, as well as of the metal ions complexing the hexamer, has been the subject of extensive investigations. This study explores the effect of helix-enhanci …91Cite
Bernardes CF, Meyer-Fernandes JR, Saad-Nehme J, Vannier-Santos MA, Peres-Sampaio CE, Vercesi AE.Int J Biochem Cell Biol. 2000 May;32(5):519-27. doi: 10.1016/s1357-2725(00)00002-9.PMID: 10736567
The presence of EGTA prevented the elimination of the mitochondrial membrane potential (DeltaPsi), supporting previous data suggesting that the binding of Ca(2+) to the mitochondrial membrane exposes buried thiols to react with DIDS. This thiocyanate derivative was …92Cite
Utschig LM, Poluektov O, Tiede DM, Thurnauer MC.Biochemistry. 2000 Mar 21;39(11):2961-9. doi: 10.1021/bi991800t.PMID: 10715116
The coordination environments of two distinct metal sites on the bacterial photosynthetic reaction center (RC) protein were probed with pulsed electron paramagnetic resonance (EPR) spectroscopy. …Thus, metal and EPR analyses confirm that we have developed reliable …93Cite
Elnour A, Hambraeus L, Eltom M, Dramaix M, Bourdoux P.Am J Clin Nutr. 2000 Jan;71(1):59-66. doi: 10.1093/ajcn/71.1.59.PMID: 10617947
Although serum thyroxine and triiodothyronine concentrations were within reference ranges, the median thyrotropin concentration was 3.78 mIU/L and 44% of the children had thyrotropin concentrations above normal. The mean urinary thiocyanate concentration was high (259 +/- …94Cite
Bolognesi M, Boffi A, Coletta M, Mozzarelli A, Pesce A, Tarricone C, Ascenzi P.J Mol Biol. 1999 Aug 20;291(3):637-50. doi: 10.1006/jmbi.1999.2975.PMID: 10448042
Thermodynamics and kinetics for cyanide, azide, thiocyanate and imidazole binding to recombinant ferric Vitreoscilla sp. homodimeric hemoglobin (Vitreoscilla Hb) have been determined at pH 6.4 and 7.0, and 20.0 degrees C, in solution and in the crystalline state. .. …95Cite
Nakasako M, Odaka M, Yohda M, Dohmae N, Takio K, Kamiya N, Endo I.Biochemistry. 1999 Aug 3;38(31):9887-98. doi: 10.1021/bi982753s.PMID: 10433695
The amino acid residues hydrogen-bonded to those hydration water molecules are highly conserved among all known nitrile hydratases and even in the homologous enzyme, thiocyanate hydrolase, suggesting the structural conservation of the water molecules in the NHase family. . …96Cite
Adak S, Bandyopadhyay U, Bandyopadhyay D, Banerjee RK.Biochemistry. 1998 Dec 1;37(48):16922-33. doi: 10.1021/bi980899l.PMID: 9836585
Among various mono- and dihydroxyl aromatic donors tested, only epinephrine shows the oxidase reaction. Binding studies indicate that epinephrine interferes with the binding of CN-, SCN-, and guaiacol indicating that HRP preferentially binds epinephrine near …97Cite
Berardi S, Stieger B, Wachter S, O’Neill B, Krahenbühl S.Hepatology. 1998 Aug;28(2):521-5. doi: 10.1002/hep.510280232.PMID: 9696019
Investigation of several sodium salts revealed that an overshoot could only be observed in the presence of chloride, but not of nitrate, thiocyanate, sulfate, or gluconate. An osmolarity plot in the presence of sodium chloride revealed a slope different from zero and a pos …98Cite
Adak S, Banerjee RK.Biochem J. 1998 Aug 15;334 ( Pt 1)(Pt 1):51-6. doi: 10.1042/bj3340051.PMID: 9693101 Free PMC article.
The reconstituted enzyme oxidizes neither guaiacol (aromatic electron donor) nor iodide or thiocyanate (inorganic donor). Although the reconstituted enzyme binds guaiacol with a similar Kd (13 mM) to that of the native enzyme (10 mM), the Kd for SCN- binding …99Cite
Transport mechanisms for iron and other transition metals in rat and rabbit erythroid cells.
Savigni DL, Morgan EH.J Physiol. 1998 May 1;508 ( Pt 3)(Pt 3):837-50. doi: 10.1111/j.1469-7793.1998.837bp.x.PMID: 9518737 Free PMC article.
The Km and Vmax values and the effects of incubation temperature and metabolic inhibitors were similar for all the metals. NaHCO3 and NaSCN increased the uptake of Zn2+ and Cd2+ but not the other metals. 4. …It is concluded that the high- and low-affinity ‘iron’ t …100Cite
The function of the periplasmic Sud protein in polysulfide respiration of Wolinella succinogenes.
Klimmek O, Kreis V, Klein C, Simon J, Wittershagen A, Kröger A.Eur J Biochem. 1998 Apr 1;253(1):263-9. doi: 10.1046/j.1432-1327.1998.2530263.x.PMID: 9578485 Free article.
Sud modified by a C-terminal His-tag (Sud-His6) was produced in Escherichia coli by expression of the sud gene. Sud-His6 catalyzed thiocyanate formation from cyanide and polysulfide. The Vmax of this activity was more than one order of magnitude higher than that of sulfide …101Cite
Daniel R, Caminade E, Martel A, Le Goffic F, Canosa D, Carrascal M, Abian J.J Biol Chem. 1997 Oct 24;272(43):26934-9. doi: 10.1074/jbc.272.43.26934.PMID: 9341128 Free article.
Escherichia coli dihydroorotase contains six cysteines/subunit, which are potential ligands of structural and catalytic zinc metals at protein sites of the enzyme. Specific thiol reagents modify, in nondenaturing conditions only, two of these cysteines; these two residues …102Cite
Volume regulation in NIH/3T3 cells not expressing P-glycoprotein. I. Regulatory volume decrease.
Pasantes-Morales H, Sánchez Olea R, Miranda D, Morán J.Am J Physiol. 1997 Jun;272(6 Pt 1):C1798-803. doi: 10.1152/ajpcell.1997.272.6.C1798.PMID: 9227407
Most anions were able to permeate, with a permeability ranking of nitrate > benzoate = iodide > thiocyanate > chloride > > gluconate. The pathway was permeable to neutral amino acids, with a permeability ranking of glycine > alanine > glutamate > ta …103Cite
Crystal structure of the gramicidin/potassium thiocyanate complex.
Doyle DA, Wallace BA.J Mol Biol. 1997 Mar 14;266(5):963-77. doi: 10.1006/jmbi.1996.0837.PMID: 9086274
The locations and numbers of potassium binding sites also differ considerably from the locations and numbers of caesium binding sites in the other structure. Combining information from all the cation binding sites in the two gramicidin/ion complexes produces …104Cite
Is cyanate a carbonic anhydrase substrate?
Supuran CT, Conroy CW, Maren TH.Proteins. 1997 Feb;27(2):272-8. doi: 10.1002/(sici)1097-0134(199702)27:2<272::aid-prot12>3.0.co;2-j.PMID: 9061790
In the former case it has been shown that “metal poisons” such as CN- and CNO- are not directly coordinated to the active site Zn(II) ion whereas spectroscopic studies indicate otherwise. …In this paper we prove experimentally that none of them may act as substrates of C …105Cite
Makowski GS, Davis EL, Hopfer SM.J Clin Lab Anal. 1997;11(2):87-93. doi: 10.1002/(sici)1098-2825(1997)11:2<87::aid-jcla4>3.0.co;2-h.PMID: 9058242 Free PMC article.
Amplification of DNA from whole blood collected on Guthrie card filter paper presents considerable technical obstacles due to the presence of natural PCR inhibitors (protein, heavy metals, heme, and heme degradation products) and low copy number of genomic material. For th …106Cite
Effect of activation of protein phosphatase 1 on sulfhydryl reactivity.
Chu Y, Lee EY, Reimann EM, Wilson SE, Schlender KK.Arch Biochem Biophys. 1996 Oct 1;334(1):83-8. doi: 10.1006/abbi.1996.0432.PMID: 8837742 Free article.107Cite
Connern CP, Halestrap AP.Biochemistry. 1996 Jun 25;35(25):8172-80. doi: 10.1021/bi9525177.PMID: 8679570
Conditions known to increase the mitochondrial NADH/NAD+ ratio decreased CyP binding. In contrast, the effects of mitochondrial membrane potential, matrix pH, and adenine nucleotide translocase conformation on the sensitivity of the MTP to [Ca2+] were not associated with a …108Cite
In vitro and in vivo inactivation of transcobalamin II receptor by its antiserum.
Bose S, Komorowski R, Seetharam S, Gilfix B, Rosenblatt DS, Seetharam B.J Biol Chem. 1996 Feb 23;271(8):4195-200. doi: 10.1074/jbc.271.8.4195.PMID: 8626762 Free article.
Exogenously added transcobalamin II-[57Co]cyanocobalamin bound very poorly (2-5%) to the affected rabbit liver, kidney, and intestinal total or intestinal basolateral membrane extracts relative to the binding by membrane extracts from normal rabbit tissues. The activity wa …109Cite
Bonander N, Karlsson BG, Vänngård T.Biochemistry. 1996 Feb 20;35(7):2429-36. doi: 10.1021/bi9522110.PMID: 8652586
The Gly and Asp mutants bind azide less strongly than the Ala mutant, due to competition with water (Gly) and the polar side chain (Asp). Similar trends are found for thiocyanate. Cyanide binds equally well to the Ala and Val mutants. A number of other small potenti …110Cite
Enzymatic determination of sodium and chloride in sweat.
Taylor RP, James TJ.Clin Biochem. 1996 Feb;29(1):33-7. doi: 10.1016/0009-9120(95)02003-9.PMID: 8929821
The sweat, collected with the Macroduct system, was diluted no more than five-fold for the volumes obtained of 16 to 80 mu L, median 32.5 mu L. The sodium assay utilized a sodium-binding cryptand to maximize linearity. RESULTS: Between-run coefficients of variation (%) at …111Cite
A unitary non-NMDA receptor short subunit from Xenopus: DNA cloning and expression.
Ishimaru H, Kamboj R, Ambrosini A, Henley JM, Soloviev MM, Sudan H, Rossier J, Abutidze K, Rampersad V, Usherwood PN, Bateson AN, Barnard EA.Recept Channels. 1996;4(1):31-49.PMID: 8723645
Each agonist shows the same KI value against [3H] kainate and [3H] AMPA binding, suggesting a common agonist site, but two conformations thereof are distinguishable by their different affinities for the antagonist NBQX and by the allosteric effect of thiocyanate ani …112Cite
Anions modulate cation-induced responses of single units of the frog glossopharyngeal nerve.
Kitada Y.Brain Res. 1995 Oct 2;694(1-2):253-63. doi: 10.1016/0006-8993(95)00779-p.PMID: 8974652
We hypothesize that anions can modulate the efficacy of cation transduction by binding to a membrane element that interacts with each of the three distinct receptors for Ca2+, Mg2+ and Na+ ions without altering the affinities of these receptors for the respective cations. …113Cite
Phospholipase A2 down-regulates the affinity of [3H]AMPA binding to rat cortical membranes.
Dev KK, Honoré T, Henley JM.J Neurochem. 1995 Jul;65(1):184-91. doi: 10.1046/j.1471-4159.1995.65010184.x.PMID: 7540662
The effects of exogenous phospholipase A2 on the binding of alpha-[3H]amino-3-hydroxy-5-methylisoxazole-4-propionate ([3H]AMPA) to rat cortical membranes in the presence of the chaotrope potassium thiocyanate were assessed. …In contrast to the effects on [3H]AMPA …114Cite
Seravalli J, Kumar M, Lu WP, Ragsdale SW.Biochemistry. 1995 Jun 20;34(24):7879-88. doi: 10.1021/bi00024a012.PMID: 7794899
Thiocyanate acted as a mixed partial inhibitor with respect to CO. The inhibition constants were pH and temperature dependent. The pH dependencies of the inhibition constants gave pKa values of approximately 7.7. Binding of thiocyanate to the oxidized form of …115Cite
Spectroscopic investigations on the highly purified lactoperoxidase Fe(III)-heme catalytic site.
Ferrari RP, Laurenti E, Cecchini PI, Gambino O, Sondergaard I.J Inorg Biochem. 1995 May 1;58(2):109-27. doi: 10.1016/0162-0134(94)00041-8.PMID: 7769383
Furthermore, the interaction of LPO with its native substrate (the thiocyanate anion), which previously was shown by NMR and EPR (at high substrate concentration) spectroscopies, has been confirmed by EPR at low temperature and low substrate concentration and by optical sp …116Cite
Caesium-binding sites in the gramicidin pore.
Doyle DA, Wallace BA.Biochem Soc Trans. 1994 Nov;22(4):1043-5. doi: 10.1042/bst0221043.PMID: 7535256 No abstract available.117Cite
Stim J, Bernardo AA, Kear FT, Qiu YY, Arruda JA.J Membr Biol. 1994 May;140(1):39-46. doi: 10.1007/BF00234484.PMID: 8051692
In the presence of the substrates Na+ and HCO3-, PITC binds covalently to amino groups unprotected by substrates leaving the Na+/HCO3- cotransporter available for specific labeling with FITC. …118Cite
Wu X, Gutierrez MM, Giacomini KM.Biochim Biophys Acta. 1994 Apr 20;1191(1):190-6. doi: 10.1016/0005-2736(94)90248-8.PMID: 8155674
Na+/thymidine uptake was not significantly affected by replacing chloride with either thiocyanate or sulfate. The Km of Na+/guanosine uptake was 149, 85.2 and 30.5 microM in the presence of a 25, 50 and 100 mM Na+ gradient, respectively, whereas the Vmax was unaffected, su …119Cite
Binding of cyanide, cyanate, and thiocyanate to human carbonic anhydrase II.
Peng Z, Merz KM Jr, Banci L.Proteins. 1993 Oct;17(2):203-16. doi: 10.1002/prot.340170209.PMID: 8265567
Based on these results we are able to explain the observed crystallographic behavior of cyanate and, by inference, thiocyanate….120Cite
Desnoyers L, Manjunath P.Arch Biochem Biophys. 1993 Sep;305(2):341-9. doi: 10.1006/abbi.1993.1431.PMID: 8373172
We have recently shown that the major proteins from bovine seminal plasma, namely BSP-A1, BSP-A2, BSP-A3, and BSP-30-kDa (collectively called BSP proteins) are novel phospholipid-binding proteins. These proteins show a Ca(2+)-independent interaction with phosphorylcholine …121Cite
Lukat GS, Doran MB, Utschig LM, Goff HM.J Inorg Biochem. 1993 May 15;50(3):157-71. doi: 10.1016/0162-0134(93)80022-2.PMID: 8388915
Interaction of lactoperoxidase with thiocyanate ion was monitored by NMR and EPR spectroscopy. Proton NMR spectra of lactoperoxidase in the presence of excess thiocyanate ion illustrated the retention of a high-spin ferric configuration consistent with predominant …122Cite
Evidence for two histidine ligands at the diiron site of methane monooxygenase.
Smith DD, Dalton H.Eur J Biochem. 1992 Dec 1;210(2):629-33. doi: 10.1111/j.1432-1033.1992.tb17463.x.PMID: 1459145 Free article.123Cite
Bubacco L, Magliozzo RS, Beltramini M, Salvato B, Peisach J.Biochemistry. 1992 Sep 29;31(38):9294-303. doi: 10.1021/bi00153a024.PMID: 1327111
A binuclear cobalt derivative of arthropod hemocyanin (Hc) has been prepared by the reaction of apo-Hc with Co(II) in the presence of thiocyanate. The crude product of the reaction contains specifically and adventitiously bound metal, the latter being removable by E …124Cite
Daniel H, Rehner GI.J Nutr. 1992 Jul;122(7):1454-61. doi: 10.1093/jn/122.7.1454.PMID: 1619472
Uptake of riboflavin into the membrane vesicles was predominantly the result of transport into an osmotically reactive space. Binding of the substrate to the membrane surface increased as a function of time and with lowering of the incubation buffer pH. …Because uptake o …125Cite
Zeiske W, Schröder H, Alpert G.J Comp Physiol B. 1992;162(4):331-9. doi: 10.1007/BF00260760.PMID: 1324259
Basolateral, but not apical, chloride, bromide and iodide stimulate IK when compared to the baseline current recorded with gluconate-, nitrate- or thiocyanate-containing salines. So-called “Cl(-)-specific” transport inhibitors (frusemide, 9-anthracene carboxylic acid, diph …126Cite
Upchurch HF, Conway E, Patterson MK Jr, Maxwell MD.J Cell Physiol. 1991 Dec;149(3):375-82. doi: 10.1002/jcp.1041490304.PMID: 1683874
Binding occurred equally well at 4 degrees C or 37 degrees C. Prior incubation of exogenous TGase with guanosine 5′-triphosphate (GTP), guanosine 5′-diphosphate (GDP), or adenosine triphosphate (ATP) had little effect on the amount bound to matrix, but prior treatment with …127Cite
Bracete AM, Sono M, Dawson JH.Biochim Biophys Acta. 1991 Nov 15;1080(3):264-70. doi: 10.1016/0167-4838(91)90012-o.PMID: 1954234
The present MCD study has been extended to include new ligands, NO, thiocyanate and cyanate, which bind to ferric BrCN-Mb. With exogenous ligands such as CO, NO and thiocyanate, the coordination structures of the BrCN-Mb complexes are similar to those of the respect …128Cite
Voltage-dependent phosphate transport in osteoblast-like cells.
Luong KV, Green J, Kleeman CR, Yamaguchi DT.J Bone Miner Res. 1991 Nov;6(11):1161-5. doi: 10.1002/jbmr.5650061104.PMID: 1805540129Cite
David P, Karlish SJ.J Biol Chem. 1991 Aug 15;266(23):14896-902.PMID: 1651313 Free article.
Lanthanides are useful probes in Ca2+ binding proteins, including sarcoplasmic reticulum (Ca2+,Mg2+)-ATPase. Here, we report that lanthanides compete with Rb+ and Na+ for occlusion in renal (Na+,K+)-ATPase. …In the future, lanthanides bound to monovalent cation sites wit …130Cite
Covalent modification of the amine transporter with N,N’-dicyclohexylcarbodiimide.
Suchi R, Stern-Bach Y, Gabay T, Schuldiner S.Biochemistry. 1991 Jul 2;30(26):6490-4. doi: 10.1021/bi00240a020.PMID: 2054348
A study of the mechanism of inhibition is presented together with the demonstration of covalent modification of the protein. DCC inhibits binding of R1 (reserpine) and R2 (tetrabenazine) types of ligands to the transporter as well as transport. Ligands of the R2 type, but …131Cite
Hueso-Ureña F, Moreno-Carretero MN, Salas-Peregrín JM, Alvarez de Cienfuegos-López G.J Inorg Biochem. 1991 Jul;43(1):17-27. doi: 10.1016/0162-0134(91)84065-h.PMID: 1940899
Seven complexes containing neutral isoorotic and 2-thioisoorotic acids, as well as thiocyanate and chloride anions as lignands, have been synthesized and characterized by means of both spectral (IR, 1H, and 13C NMR) and thermal (TG and DSC) methods, as well as conductivity …132Cite
Gavva SR, Harris BG, Weiss PM, Cook PF.Biochemistry. 1991 Jun 11;30(23):5764-9. doi: 10.1021/bi00237a019.PMID: 2043616
A thiol group at the malate-binding site of the NAD-malic enzyme from Ascaris suum has been modified to thiocyanate. The modified enzyme generally exhibits slight increases in KNAD and Ki metal and decreases in Vmax as the metal size increases from Mg2 …133Cite
Technetium labeling of monoclonal antibodies with functionalized BATOs. 1. TcCl(DMG)3PITC.
Linder KE, Wen MD, Nowotnik DP, Malley MF, Gougoutas JZ, Nunn AD, Eckelman WC.Bioconjug Chem. 1991 May-Jun;2(3):160-70. doi: 10.1021/bc00009a005.PMID: 1932215
B72.3 labeled with 99mTcCl(DMG)3PITC displayed high binding to a TAG 72 affinity column and had a distribution in normal mice similar to that reported for iodine-labeled B72.3….134Cite
Faller LD, Diaz RA, Scheiner-Bobis G, Farley RA.Biochemistry. 1991 Apr 9;30(14):3503-10. doi: 10.1021/bi00228a022.PMID: 1849428
Stopped-flow fluorometry has been used to measure the forward and reverse rates of the conformational change from E1 to E2 in the fluorescein-modified proton and sodium pumps (1) as a function of Na+ and K+ concentrations to verify the proposed mechanism of ion interaction with t …135Cite
Actin dynamics studied by solid-state NMR spectroscopy.
Phillips L, Separovic F, Cornell BA, Barden JA, dos Remedios CG.Eur Biophys J. 1991;19(3):147-55. doi: 10.1007/BF00185455.PMID: 2060494
The dynamics of probes attached to dry cysteine powder or F-actin were very similar and the binding of myosin had little effect indicating that the local probe environment imposes the major influence on motion in the solid state. …136Cite
Comparison of the red blood cell Ca2(+)-ATPase in ghost membranes and after purification.
Kosk-Kosicka D.Mol Cell Biochem. 1990 Dec 20;99(2):75-81. doi: 10.1007/BF00230336.PMID: 2149586
Finally, stoichiometry of the formation of phosphorylated enzyme intermediate (EP) and titrations of the ATP binding region with fluorescein 5′-isothiocyanate (FITC) were characterized. For the first time a high phosphorylation level of 2.0-2.4 mmol EP/mg of purified enzym …137Cite
Pola E, Bertran J, Roca A, Palacín M, Zorzano A, Testar X.Biochem J. 1990 Oct 15;271(2):297-303. doi: 10.1042/bj2710297.PMID: 2241916 Free PMC article.138Cite
Jona I, Matko J, Martonosi A.Biochim Biophys Acta. 1990 Oct 5;1028(2):183-99. doi: 10.1016/0005-2736(90)90153-f.PMID: 2145977
Structural fluctuations were also evident in the energy transfer between FITC linked to the nucleotide-binding domain and Nd3+ bound at the putative Ca2+ sites. By contrast the normalized energy transfer efficiency between AEDANS and Pr3+ was relatively insensitive to temp …139Cite
Dux L, Green HJ, Pette D.Eur J Biochem. 1990 Aug 28;192(1):95-100. doi: 10.1111/j.1432-1033.1990.tb19200.x.PMID: 2144818 Free article.
Confirming previous results, fluorescein isothiocyanate (FITC) binding to the enzyme of the chronically stimulated muscle was markedly reduced. The FITC-labeled fraction of the enzyme from both the normal and the stimulated muscle followed similar time courses of tryptic c …140Cite
Alkaline serine proteinase from Thermomonospora fusca YX. Stability to heat and denaturants.
Kristjansson MM, Kinsella JE.Biochem J. 1990 Aug 15;270(1):51-5. doi: 10.1042/bj2700051.PMID: 2396992 Free PMC article.
Although some stabilization occurs upon increasing ionic strength, Ca2+ binding does not appear to play a role in thermal stability. Denaturants, i.e. 8 M-urea, 6 M-guanidinium chloride or 1% SDS, had no significant effect on the activity of the enzyme after 24 h at 25 deg …141Cite
Studies of zinc transport into brush-border membrane vesicles isolated from pig small intestine.
Tacnet F, Watkins DW, Ripoche P.Biochim Biophys Acta. 1990 May 24;1024(2):323-30. doi: 10.1016/0005-2736(90)90361-q.PMID: 2112950
With highly permeant anions such as thiocyanates, zinc uptake was considerably augmented, suggesting a movement of zinc in a complexed form involving the presence of negative species. …142Cite
Halestrap AP, Davidson AM.Biochem J. 1990 May 15;268(1):153-60. doi: 10.1042/bj2680153.PMID: 2160810 Free PMC article.
Titration of the rate of swelling with increasing concentrations of cyclosporin showed the number of cyclosporin-binding sites (+/- S.E.M.) in liver and heart mitochondria to be respectively 113.7 +/- 5.0 (n = 9) and 124.3 +/- 11.2 (n = 10) pmol/mg of protein, with a Ki of …143Cite
Modi S, Saxena AK, Behere DV, Mitra S.Biochim Biophys Acta. 1990 Apr 19;1038(2):164-71. doi: 10.1016/0167-4838(90)90200-y.PMID: 2158822
The binding of thiocyanate and cyanide was shown to be away from the metal center and the distance of the 15N of thiocyanate and cyanide from the paramagnetic manganese ion in Mn(III)HRP was found to be 6.9 and 6.6 A, respectively. Except for cyanide …144Cite
Ronzani N, Hasselbach W, Stephan L.Eur J Biochem. 1990 Mar 30;188(3):557-65. doi: 10.1111/j.1432-1033.1990.tb15436.x.PMID: 2139606 Free article.
Under pressure and in the presence of calcium, A1 and A2 rapidly disappear indicating the presence of calcium-binding sites in these peptides. In contrast, the B1-3 peptides which are most likely derivates of the B fragment accumulate in the presence and absence of calcium …145Cite
Lepock JR, Rodahl AM, Zhang C, Heynen ML, Waters B, Cheng KH.Biochemistry. 1990 Jan 23;29(3):681-9. doi: 10.1021/bi00455a013.PMID: 2140054
Two fluorophores were monitored: intrinsic tryptophan (localized in the transmembrane region) and fluorescein isothiocyanate (FITC)-labeled Lys-515 (located in the nucleotide binding domain). Inactivation, defined as loss of activity, and denaturation, defined as conformat …146Cite
Filippini A, Taffs RE, Agui T, Sitkovsky MV.J Biol Chem. 1990 Jan 5;265(1):334-40.PMID: 2136737 Free article.
The enzyme(s) activity is Ca2+- and Mg2+-dependent and in this respect is similar to recently described hepatic cells ecto-ATPase. We tested effects of known ATP-binding site-specific reagents fluorescein 5′-isothiocyanate (FITC) and 5′-fluorosulfonylbenzoyladenosine (FSBA …147Cite
Freyaldenhoven MP, Royer-Pokora B, Napierski I, Royer HD.Nucleic Acids Res. 1989 Nov 11;17(21):8891. doi: 10.1093/nar/17.21.8891.PMID: 2479912 Free PMC article.148Cite
Fluorescence decay of sarcoplasmic reticulum ATPase. Ligand binding and hydration effects.
Ferreira ST, Verjovski-Almeida S.J Biol Chem. 1989 Sep 15;264(26):15392-7.PMID: 2527852 Free article.
The intrinsic tryptophan fluorescence decay was complex and was fitted either with three exponentials or with bimodal Lorentzian distributions of lifetimes. Ca2+ binding to the high affinity sites in the ATPase produced an increase of 11% in the center of the main componen …149Cite
Salvato B, Beltramini M, Ricchelli F, Tallandini L.Biochim Biophys Acta. 1989 Sep 14;998(1):14-20. doi: 10.1016/0167-4838(89)90112-x.PMID: 2790051
Three cobalt derivatives of bovine erythrocyte superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) have been prepared under different pH conditions using a cobalt-thiocyanate complex which has already proved to yield specific substitutions on other cop …150Cite
Structural transition in the metal-free hexamer of protein-engineered [B13 Gln]insulin.
Wollmer A, Rannefeld B, Stahl J, Melberg SG.Biol Chem Hoppe Seyler. 1989 Sep;370(9):1045-53. doi: 10.1515/bchm3.1989.370.2.1045.PMID: 2692616
For hexamer formation of native insulin the repulsive potential of six B13 Glu carboxylate groups coming together in the centre is overcome by zinc binding to B10 His. Substitution of Gln for Glu in position B13 by site-directed mutagenesis, i.e. replacement of the repelli …151Cite
Ahlsten G, Tuvemo T, Gebre-Medhin M.Acta Paediatr Scand. 1989 Sep;78(5):671-6. doi: 10.1111/j.1651-2227.1989.tb11124.x.PMID: 2596272
Cigarette smoke exposure was verified by determinations of nicotine, cotinine and thiocyanate concentrations in cord blood. Infants of smoking mothers had a slightly lower mean birth weight (3490 +/- 430 g) than control infants (3780 +/- 460 g). …Infants of smoking mothe …152Cite
Shortle D, Meeker AK, Gerring SL.Arch Biochem Biophys. 1989 Jul;272(1):103-13. doi: 10.1016/0003-9861(89)90200-2.PMID: 2544138
Unlike GuHCl, urea exhibited a slightly reduced value of d delta Gapp/dCurea at low concentrations. Results with a number of thiocyanate, perchlorate, and iodide salts confirmed that the Hofmeister series holds for concentrations below 0.1 M; that is, with regard to effica …153Cite
Biotinylphallotoxins: preparation and use as actin probes.
Faulstich H, Zobeley S, Bentrup U, Jockusch BM.J Histochem Cytochem. 1989 Jul;37(7):1035-45. doi: 10.1177/37.7.2499619.PMID: 2499619154Cite
Saito K, Yamamoto K, Nagayama I, Uemura J, Kuzuya T.Endocrinol Jpn. 1989 Jun;36(3):325-33. doi: 10.1507/endocrj1954.36.325.PMID: 2555140 Free article.
The one is preservation of the Na+-dependent I- transport activity by protecting a sulfhydryl group, and the other is reduction of nonspecific I- binding to P-vesicles. In addition, C1O4- is a more specific inhibitor of thyroid I- transport than SCN-, when non-specific I- …155Cite
Carraway KL 3rd, Koland JG, Cerione RA.J Biol Chem. 1989 May 25;264(15):8699-707.PMID: 2498317 Free article.
Both of these derivatives were shown to be active in binding to membrane receptors and in the activation of the endogenous receptor/tyrosine kinase activity. …However, divalent metal ion activators of the EGF receptor/tyrosine kinase caused a significant increase …156Cite
Tait JF, Gibson D, Fujikawa K.J Biol Chem. 1989 May 15;264(14):7944-9.PMID: 2524476 Free article.
Human placental anticoagulant protein-I (PAP-I) is a member of the lipocortin/calpactin/annexin family of Ca2+-dependent phospholipid binding proteins. PAP-I was labeled with fluorescein 5-isothiocyanate (1 mol/mol); this derivative had anticoagulant activity identical to …157Cite
Fowler C, Huggins JP, Hall C, Restall CJ, Chapman D.Biochim Biophys Acta. 1989 Apr 28;980(3):348-56. doi: 10.1016/0005-2736(89)90323-4.PMID: 2540839
The data are more consistent with phospholamban associating with the Ca2+-ATPase following phosphorylation, or more complex models in which only the hydrophilic domain of phospholamban binds with and dissociates from the Ca2+-ATPase….158Cite
Friedman ML, Ball WJ Jr.Biochim Biophys Acta. 1989 Mar 16;995(1):42-53. doi: 10.1016/0167-4838(89)90231-8.PMID: 2466488
Mg2+ binding to the enzyme alters both the conformation of this epitope region and its coupling of ligand interactions. …These studies indicate that the 9-A5 epitope participates in the E1 in equilibrium E2 conformational transitions, links Na+-K+ interactions and ouabai …159Cite
Markus S, Priel Z, Chipman DM.Biochemistry. 1989 Jan 24;28(2):793-9. doi: 10.1021/bi00428a057.PMID: 2523730
The vanadate species bound to the enzyme is clearly monomeric orthovanadate, probably H2VO4-. Vanadate binding is noncooperative, suggesting an absence of interactions between the Ca2+-ATPase subunits. …On the basis of the fluorescence properties of this complex, it is s …160Cite
The fluorescein isothiocyanate-binding site of the plasma-membrane H+-ATPase of Neurospora crassa.
Pardo JP, Slayman CW.J Biol Chem. 1988 Dec 15;263(35):18664-8.PMID: 2904434 Free article.
The mammalian (Na+,K+), Ca2+-, and (H+,K+)-ATPases contain a well-characterized lysine residue that reacts with fluorescein 5′-isothiocyanate (FITC); enzymatic activity is protected by ATP, suggesting that the residue is located in or near the nucleotide-binding domain. In …161Cite
Thiocyanate stabilizes AMPA binding to the quisqualate receptor.
Nielsen EO, Cha JH, Honoré T, Penney JB, Young AB.Eur J Pharmacol. 1988 Nov 22;157(2-3):197-203. doi: 10.1016/0014-2999(88)90383-4.PMID: 2906292 Free article.
In contrast, calcium and chloride had little effect on the binding of [3H](RS)-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid ([3H]AMPA), but 100 mM thiocyanate stimulated binding 4-fold. AMPA displaced little [3H]glutamate binding from quisq …162Cite
Champeil P, Riollet S, Orlowski S, Guillain F, Seebregts CJ, McIntosh DB.J Biol Chem. 1988 Sep 5;263(25):12288-94.PMID: 2970458 Free article.
Acceleration in the absence of Mg2+ was observed at relatively low concentrations of ATP and 8-bromo-ATP (0.01-0.1 mM) and binding of metal-free ATP and ADP, but not Mg.ATP, to the phosphoenzyme in this concentration range was demonstrated directly. Modification of …163Cite
Calcium-activated neutral proteases (calpains) are carbohydrate binding proteins.
Zimmerman UJ, Schlaepfer WW.J Biol Chem. 1988 Aug 25;263(24):11609-12.PMID: 2841330 Free article.
Other calcium binding proteins, including calmodulin, calpastatin, casein, and neurofilament proteins, fail to bind under the same conditions. …When the enzyme subunits are dissociated in 1 M NaSCN, only the 30-kDa subunit binds to the agarose matrix. Furthermore, …164Cite
Miki M, dos Remedios CG.J Biochem. 1988 Aug;104(2):232-5. doi: 10.1093/oxfordjournals.jbchem.a122448.PMID: 3141394 Free article.
The resonance energy transfer between fluorescein-5-isothiocyanate (FITC) attached to Lys-61 and Co2+ bound to the high-affinity metal binding site was measured. The distance between FITC and Co2+ on the actin molecule was calculated to be either 1.9 nm, using the a …165Cite
Ferreira ST, Verjovski-Almeida S.J Biol Chem. 1988 Jul 15;263(20):9973-80.PMID: 2968343 Free article.
The data indicate that a peptide sequence of fragment A2 is involved in the binding of the nucleoside moiety of UTP and possibly belongs to the nucleotide domain of the ATPase in addition to the sequence of fragment A1 which contains the phosphorylation residue….166Cite
Lewinsohn DM, Nickoloff BJ, Butcher EC.J Immunol Methods. 1988 May 25;110(1):93-100. doi: 10.1016/0022-1759(88)90087-7.PMID: 3131438
We describe the effect of different time and temperature incubations of FITC-labeled cells on fluorescence intensity, and we use the method to analyze the binding of peripheral blood mononuclear leukocytes to interferon-gamma-treated keratinocytes. …167Cite
Meers P, Feigenson GW.Biochim Biophys Acta. 1988 Mar 3;938(3):469-82. doi: 10.1016/0005-2736(88)90145-9.PMID: 3349073 Free article.
These resonances were used to examine the location, orientation and ion-binding of membrane-bound valinomycin. The order of affinity of membrane-bound valinomycin for cations is Rb+ greater than K+ greater than Cs+ greater than Ba2+, and binding is sensitive to surf …168Cite
Interaction of anions with the active site of carboxypeptidase A.
Bicknell R, Schäffer A, Bertini I, Luchinat C, Vallee BL, Auld DS.Biochemistry. 1988 Feb 9;27(3):1050-7. doi: 10.1021/bi00403a030.PMID: 2835074
The cobalt electronic absorption spectrum is insensitive to azide binding at the first site but shows marked changes upon azide binding to the second site. Thus, azide elicits a spectral change with new lambda max (epsilon M) values of 590 (330) and 540 nm (190) and …169Cite
The mechanism of peroxidase-mediated cytotoxicity. II. Role of the heme moiety.
Lin H, McFaul SJ, Brady JC, Everse J.Proc Soc Exp Biol Med. 1988 Jan;187(1):7-13. doi: 10.3181/00379727-187-42629.PMID: 3340620
This conclusion was confirmed by demonstrating that microperoxidase, hemin, and hematoheme also exert cytotoxic activity in the presence of hydrogen peroxide and iodide, the kinetics of which were identical to those obtained with the peroxidases. Fluoride, bromide, and thiocya …170Cite
Eriksson AE, Kylsten PM, Jones TA, Liljas A.Proteins. 1988;4(4):283-93. doi: 10.1002/prot.340040407.PMID: 3151020
The binding of four inhibitors–mercuric ion, 3-acetoxymercuri-4-aminobenzenesulfonamide (AMS), acetazolamide (Diamox), and thiocyanate ion–to human carbonic anhydrase II (HCA II) has been studied with X-ray crystallography. …One of these sites is pointing toward …171Cite
Binding of calcium to the vanadate complex of FITC-labelled Ca-ATPase.
Markus S, Priel Z, Chipman DM.Prog Clin Biol Res. 1988;273:171-6.PMID: 2971226 No abstract available.172Cite
Transformation of the [3H]aldosterone-type I receptor complex to a DNA-binding species.
Luttge WG, Emadian SM.Life Sci. 1988;43(10):837-43. doi: 10.1016/0024-3205(88)90510-3.PMID: 2970566
For most steroid receptor complexes, the transformation to a DNA-binding species can be achieved readily in vitro by incubation at elevated temperatures and/or salt concentrations. Although the aldosterone-Type I receptor complex forms a clear exception to this generalizat …173Cite
Gandhi CR, Ross DH.J Neurochem. 1988 Jan;50(1):248-56. doi: 10.1111/j.1471-4159.1988.tb13257.x.PMID: 2961847174Cite
Gengo P, Su CM, Yousif FB, Triggle DJ.Can J Physiol Pharmacol. 1987 Dec;65(12):2472-82. doi: 10.1139/y87-392.PMID: 3449204
In vivo administration of o-NCS-DHP (5-25 mg/kg) produced a persistent reduction of [3H]nitrendipine binding in rat brain, gut, and heart characterized as Bmax, but not KD, changes. No effects on [3H]dihydroalprenolol or [3H]quinuclidinyl benzilate binding were dete …175Cite
Nucleotide binding sites and chemical modification of the chromaffin granule proton ATPase.
Moriyama Y, Nelson N.J Biol Chem. 1987 Oct 25;262(30):14723-9.PMID: 2889730 Free article.
The purified enzyme was reconstituted as a highly active proton pump, and the binding of N-ethylmaleimide and nucleotides to individual subunits was studied. N-Ethylmaleimide binds to subunits I, II, and IV, but inhibition of both ATPase and proton pumping activity …176Cite
Festin R, Björklund B, Tötterman TH.J Immunol Methods. 1987 Jul 16;101(1):23-8. doi: 10.1016/0022-1759(87)90211-0.PMID: 2956330177Cite
Peerce BE, Wright EM.Biochemistry. 1987 Jul 14;26(14):4272-9. doi: 10.1021/bi00388a014.PMID: 3663588
In the first, we have measured the effect of Na+ on the binding of fluorescein isothiocyanate (FITC) to the glucose site; 100 mM Na increased the specific [blocked by D-glucose, p-(chloromercuri)benzenesulfonic acid, and N-acetylimidazole] FITC binding to a 75-kilod …178Cite
Kinetics and mechanism of anionic ligand binding to carbonic anhydrase.
Prabhananda BS, Rittger E, Grell E.Biophys Chem. 1987 May 9;26(2-3):217-24. doi: 10.1016/0301-4622(87)80024-8.PMID: 3111557
These kinetic results strongly indicate that the deprotonated anion binds to an unoccupied coordination position of the protein-bound heavy metal ion in the form of an addition reaction. Upon binding of the anion, the coordination number of the Co2+ in the ac …179Cite
Sawaya A, Deyme M, Benoit JP.Biomaterials. 1987 May;8(3):211-6. doi: 10.1016/0142-9612(87)90066-4.PMID: 3607154
With strongly crosslinked albumin, the Scatchard plots of equilibrium isotherms indicate a maximum number of binding sites for Ca2+ ions, when N is equal to 12 and an apparent association constant, K’ is equal to 7.8 X 10(3) l/mol. …180Cite
Inhibition of rat liver microsomal Ca2+-ATPase by fluorescein-5′-isothiocyanate.
Fleschner CR, Kraus-Friedmann N.Arch Biochem Biophys. 1987 May 1;254(2):448-53. doi: 10.1016/0003-9861(87)90123-8.PMID: 2953307
These data taken together are consistent with the proposal that fluorescein-5′-isothiocyanate binds the Ca2+-ATPase ATP-binding site, suggesting the presence of a lysine residue in this domain. …181Cite
Ferry DR, Goll A, Glossmann H.Biochem J. 1987 Apr 1;243(1):127-35. doi: 10.1042/bj2430127.PMID: 2440422 Free PMC article.
The arylazide 1,4-dihydropyridine (-)-[3H]azidopine binds to a saturable population of sites in guinea-pig heart membranes with a dissociation constant (KD) of 30 +/- 7 pM and a density (Bmax.) of 670 +/- 97 fmol/mg of protein. This high-affinity binding site is ass …182Cite
Papain fragmentation of the gastric (H+ + K+)-ATPase.
Saccomani G, Mukidjam E.Biochim Biophys Acta. 1987 Mar 18;912(1):63-73. doi: 10.1016/0167-4838(87)90248-2.PMID: 3030430
With further exposure to papain the 77 kDa peptide became fragmented into a 28 kDa soluble peptide that retained the phosphorylating site. Binding of fluorescein 5′-isothiocyanate (FITC) to the native enzyme did not affect the sites of papain hydrolysis because the same pe …183Cite
Ross DC, McIntosh DB.J Biol Chem. 1987 Feb 15;262(5):2042-9.PMID: 2950084 Free article.
Tryptic cleavage patterns of the glutaraldehyde-modified enzyme were consistent with covalent linkage of A1 and B fragments that have been postulated to comprise the phosphorylation and nucleotide-binding domains (MacLennan, D. H., Brandt, C. J., Korczak, B., and Green, N. …184Cite
Leberer E, Härtner KT, Pette D.Eur J Biochem. 1987 Feb 2;162(3):555-61. doi: 10.1111/j.1432-1033.1987.tb10675.x.PMID: 2951251 Free article.
Kinetic analyses demonstrated that affinities of the SR Ca2+-ATPase towards Ca2+ and ATP were unaltered. Positive cooperativity for Ca2+ binding (h = 1.5) was maintained. However, a 50% decrease in Ca2+-dependent phosphoprotein formation indicated the presence of inactive …185Cite
Teruel JA, Gómez-Fernández JC.Biochim Biophys Acta. 1986 Dec 16;863(2):178-84. doi: 10.1016/0005-2736(86)90257-9.PMID: 2947628
Measurements of fluorescence energy transfer have been performed to determine the distance between the lipid-water interface and the ATP-binding site in the (Ca2+ + Mg2+)-ATPase from sarcoplasmic reticulum. The calculated distance between the donor, FITC bound to the prote …186Cite
Nakahara M.Hinyokika Kiyo. 1986 Nov;32(11):1689-99.PMID: 3825817 Free article. Japanese.
For improved purification of human prostate androgen receptors (AR), the effect of molybdate, NaSCN and heparin on the binding capacity of human prostate cytosol AR was investigated. When 10 mM of molybdate in final concentration was added to cytosol, the increase in the m …187Cite
Distance between substrate sites on the Na-glucose cotransporter by fluorescence energy transfer.
Peerce BE, Wright EM.Proc Natl Acad Sci U S A. 1986 Nov;83(21):8092-6. doi: 10.1073/pnas.83.21.8092.PMID: 2430277 Free PMC article.
Covalent fluorescent probes were used to label the rabbit intestinal brush border Na+-glucose cotransporter at the putative glucose and Na+ binding sites, and a steady-state fluorescence energy transfer technique was used to measure the distance between the two binding …188Cite
LoBrutto R, Smithers GW, Reed GH, Orme-Johnson WH, Tan SL, Leigh JS Jr.Biochemistry. 1986 Sep 23;25(19):5654-60. doi: 10.1021/bi00367a046.PMID: 3022800
In transition-state analogue complexes of creatine kinase (enzyme-MnIIADP-anion-creatine), superhyperfine interactions from the directly coordinated nitrogen of the thiocyanate ligand give envelope modulations. The source of the modulations was confirmed by measurements wi …189Cite
1H n.m.r. studies of insulin. Reversible transformation of 2-zinc to 4-zinc insulin hexamer.
Ramesh V, Bradbury JH.Int J Pept Protein Res. 1986 Aug;28(2):146-53.PMID: 3533813
1H n.m.r. studies at 270 MHz were made of the transformation of 2 Zn insulin hexamer to 4 Zn hexamer produced by the addition of anions (thiocyanate ion). Four separate H2 histidine resonances were observed for the B5 and B10 histidines in 2 Zn hexamer at pH 7 and 9 and fo …190Cite
Arakawa T.Arch Biochem Biophys. 1986 Jul;248(1):101-5. doi: 10.1016/0003-9861(86)90405-4.PMID: 3729409
From the previously observed results of preferential interactions for salting-out salts with proteins, it was shown that the free energy of the protein is increased by addition of the salts and this unfavorable free energy is smaller for the proteins bound to the columns because …191Cite
Conformational transitions in the Ca2+ + Mg2+-activated ATPase and the binding of Ca2+ ions.
Froud RJ, Lee AG.Biochem J. 1986 Jul 1;237(1):197-206. doi: 10.1042/bj2370197.PMID: 3026311 Free PMC article.
Effects of Ca2+ as a function of pH allow an estimate of the effect of pH on the E1/E2 ratio, consistent with kinetic studies. A model is presented for binding of Ca2+ to the ATPase as a function of pH that is consistent both with the data on the E1/E2 equilibrium and with …192Cite
Amiloride-sensitive sodium-hydrogen exchange in osmotically shrunken rabbit red blood cells.
Jennings ML, Douglas SM, McAndrew PE.Am J Physiol. 1986 Jul;251(1 Pt 1):C32-40. doi: 10.1152/ajpcell.1986.251.1.C32.PMID: 2425629
Replacement of chloride with acetate does not inhibit the sodium flux, but replacement with either nitrate or thiocyanate is inhibitory. An inward sodium gradient in shrunken cells induces a net uphill efflux of acid equivalents, indicating that the sodium transport is a s …193Cite
Ionic requirements of peritubular taurine transport in Fundulus kidney.
Wolff NA, Perlman DF, Goldstein L.Am J Physiol. 1986 Jun;250(6 Pt 2):R984-90. doi: 10.1152/ajpregu.1986.250.6.R984.PMID: 3717388
In the absence of Cl- the Na+ dependence of taurine influx was reduced to a stoichiometric relationship of 1.4 Na+:1 taurine, indicating an effect of Cl- on the binding of Na+ to the transport system. A model for Na+ -Cl- -taurine cotransport is presented….194Cite
Nagamune H, Urayama O, Hara Y, Nakao M.J Biochem. 1986 Jun;99(6):1613-24. doi: 10.1093/oxfordjournals.jbchem.a135635.PMID: 3017924 Free article.
These values are consistent with their Kd values for the high-affinity ATP binding site of the Na,K-ATPase (Hegyvary, C. & Post, R.L. (1971) J. Biol. Chem. 246, 5234-5240). …On the basis of these results, it is strongly suggested that the (-K)phosphatase reaction is …195Cite
Cooperative interaction of histone H1 with DNA.
Watanabe F.Nucleic Acids Res. 1986 Apr 25;14(8):3573-85. doi: 10.1093/nar/14.8.3573.PMID: 2939395 Free PMC article.
The cooperative binding of histone H1 with DNA was studied using a fluorescently labelled histone H1. The titration data were analysed in terms of the large ligand model. …An extrapolation of K’ to 1M NaCl yielded a small value, K’ = 5 +/- 2 M-1. Thus the binding …196Cite
Ohta T, Nagano K, Yoshida M.Proc Natl Acad Sci U S A. 1986 Apr;83(7):2071-5. doi: 10.1073/pnas.83.7.2071.PMID: 3008150 Free PMC article.197Cite
Jona I, Martonosi A.Biochem J. 1986 Mar 1;234(2):363-71. doi: 10.1042/bj2340363.PMID: 2941009 Free PMC article.
The effects of Ca2+, lanthanide ions (Gd3+, La3+ and Pr3+) and membrane potential on the fluorescence of tryptophan and covalently bound fluorescein were analysed in native and fluorescein isothiocyanate (FITC)-labelled sarcoplasmic reticulum vesicles. The binding of Ca2+ …198Cite
Takeguchi N, Yamazaki Y.J Biol Chem. 1986 Feb 25;261(6):2560-6.PMID: 3005257 Free article.
Preincubation of gastric vesicles with picoprazole, which is a specific inhibitor of H,K-ATPase and binds to 100-kDa polypeptides of the enzyme, dose dependently inhibited opening of the Cl- conductance by Cu2+-o-phenanthroline, indicating that the Cl- conductance is part …199Cite
Olsnes S, Sandvig K.J Biol Chem. 1986 Feb 5;261(4):1553-61.PMID: 3944101 Free article.
The concentration of diphtheria toxin required to obtain maximal inhibition (0.3 micrograms/ml) was sufficient to ensure close to maximal toxin binding to the cells. Even in ATP-depleted cells and in the absence of permeant anions, low pH induced inhibition of anion antipo …200Cite
Nandi J, Ray TK.Arch Biochem Biophys. 1986 Feb 1;244(2):701-12. doi: 10.1016/0003-9861(86)90639-9.PMID: 3004348
Two hypotheses have recently been proposed for the thiocyanate inhibition of gastric acid secretion–a protonophore mechanism and an uncoupling model. …Gastric microsomes bind 5 nmol of SCN-/mg, and a “pure” and highly active fraction of H+,K+-ATPase prepared from gastri …
201Cite
Miyamoto H, Ikehara T, Yamaguchi H, Hosokawa K, Yonezu T, Masuya T.J Membr Biol. 1986;92(2):135-50. doi: 10.1007/BF01870703.PMID: 3761359
Results suggested that one unit molecule of this transport system has one Na+, one K+ and two Cl- sites with different affinities, one of the Cl- sites related with binding of Na+, and the other with binding of K+(Rb+). A 1:1 stoichiometry was demonstrated between o …202Cite
Tu CK, Silverman DN.Biochemistry. 1985 Oct 8;24(21):5881-7. doi: 10.1021/bi00342a029.PMID: 3936538
These results are applied to models derived from proton-relaxation enhancement of water exchanging from the inner coordination shell of the cobalt in carbonic anhydrase. The anions iodide, cyanate, and thiocyanate inhibited catalysis of 18O exchange by Co(II)-substituted c …203Cite
Csermely P, Varga S, Martonosi A.Eur J Biochem. 1985 Aug 1;150(3):455-60. doi: 10.1111/j.1432-1033.1985.tb09043.x.PMID: 3160591 Free article.
Monovanadate binds to the Ca2+-transport ATPase at a single high affinity site (site 1), that is presumably related to the binding site for inorganic orthophosphate, and to one of the two sites for decavanadate. …Decavanadate also binds with high affinity t …204Cite
Highsmith S, Barker D, Scales DJ.Biochim Biophys Acta. 1985 Jul 11;817(1):123-33. doi: 10.1016/0005-2736(85)90074-4.PMID: 3159430
The Ca2+ and Mg2+ dependencies for high-affinity vanadate binding were similar but not identical to those for orthophosphate. In addition, it was found that there is low-affinity (Ka = 380 M-1) vanadate binding, which causes a 25% decrease in fluorescence. The Ca2+ …205Cite
The role of transferrin and ferritin in the fetal-maternal-placental unit.
Okuyama T, Tawada T, Furuya H, Villee CA.Am J Obstet Gynecol. 1985 Jun 1;152(3):344-50. doi: 10.1016/s0002-9378(85)80225-8.PMID: 4003478
In both anemic and nonanemic patients serum ferritin levels decreased and total iron-binding capacity increased as gestation progressed. The total iron-binding capacity is higher in maternal than in umbilical cord blood at delivery; this suggests that little or no f …206Cite
Roy S, Galton N, Michaelis E.J Neurochem. 1985 Jun;44(6):1809-15. doi: 10.1111/j.1471-4159.1985.tb07172.x.PMID: 2859356
Antibodies (Abs) raised against the L-glutamate-binding protein (GBP) purified from bovine brain were used to define the possible physiologic activity of GBP in synaptic membranes. Three processes were examined for their sensitivity to the Abs: the excitatory amino acid st …207Cite
Evidence for tyrosyl residues at the Na+ site on the intestinal Na+/glucose cotransporter.
Peerce BE, Wright EM.J Biol Chem. 1985 May 25;260(10):6026-31.PMID: 3922968 Free article.
A tyrosine group has been identified at, or near, the Na+-binding site of the Na+/glucose and Na+/proline cotransporters of rabbit intestinal brush-borders. …All three reagents inhibited glucose transport, phlorizin binding, and fluorescein isothiocyanate quenchin …208Cite
Saito K, Yamamoto K, Takai T, Kuzuya T.J Biochem. 1985 Feb;97(2):599-604. doi: 10.1093/oxfordjournals.jbchem.a135095.PMID: 2989257 Free article.209Cite
Sodium-dependent conformational changes in the intestinal glucose carrier.
Wright EM, Peerce BE.Ann N Y Acad Sci. 1985;456:108-14. doi: 10.1111/j.1749-6632.1985.tb14852.x.PMID: 3004286 No abstract available.210Cite
Reversible dihydropyridine isothiocyanate binding to brain calcium channels.
Greenberg DA, Cooper EC, Carpenter CL.J Neurochem. 1985 Jan;44(1):319-21. doi: 10.1111/j.1471-4159.1985.tb07148.x.PMID: 2578060
Voltage-dependent calcium channels from ileal smooth muscle can be affinity-labeled with a [3H]dihydropyridine isothiocyanate radioligand. We examined the binding of this agent to brain membranes, to compare the properties of calcium channel drug binding sites in br …211Cite
Highsmith S, Murphy AJ.J Biol Chem. 1984 Dec 10;259(23):14651-6.PMID: 6238964 Free article.
Both methods indicated that Nd3+ inhibited the ATPase activity by binding in the high-affinity Ca2+ binding sites. The stoichiometry of binding was about 11 nmol of Nd3+ bound per mg of SR proteins at pNd = 6.5. At higher [Nd3+], substantial nonspecific bi …212Cite
Sodium-induced conformational changes in the glucose transporter of intestinal brush borders.
Peerce BE, Wright EM.J Biol Chem. 1984 Nov 25;259(22):14105-12.PMID: 6438091 Free article.
Competition experiments with Na+ and phlorizin or glucose indicated that FITC binds to the glucose site on the carrier. Carrier-bound FITC displays a saturable quenching of fluorescence in the presence of Na+. …FITC labeled a 75-kilodalton peptide on sodium dodecyl sulfa …213Cite
Highsmith S.Biochem Biophys Res Commun. 1984 Oct 15;124(1):183-9. doi: 10.1016/0006-291x(84)90934-3.PMID: 6238593
The CaATPase of skeletal muscle sarcoplasmic reticulum was specifically labeled in the ATP binding site with fluorescein isothiocyanate under gentle conditions (pH 7 X 5). Fluorescence energy transfer from the attached fluorescein to Nd3+ indicated that a cation binding …214Cite
Synthetic neoglycoproteins: a class of regents for detection of sugar-recognizing substances.
Kataoka M, Tavassoli M.J Histochem Cytochem. 1984 Oct;32(10):1091-8. doi: 10.1177/32.10.6434628.PMID: 6434628
Detection of the marker then indicates the presence of the sugar-binding protein. Control experiments in the presence of unlabeled neoglycoprotein or specific sugar indicated the specificity of the reaction. This method was used to analyze the kinetics of binding fo …215Cite
Moncharmont B, Anderson WL, Rosenberg B, Parikh I.Biochemistry. 1984 Aug 14;23(17):3907-12. doi: 10.1021/bi00312a018.PMID: 6207854
The 4S “high-salt” form of the estrogen receptor has one determinant per molecule, appearing to be the smallest form of the receptor not containing repetitive structures associated with the steroid binding site. The nuclear receptor also shows the presence of more than one …216Cite
Rothman RB, Danks JA, Pert CB, Jacobson AE, Burke TR Jr, Rice KC.Neuropeptides. 1984 May;4(3):261-8. doi: 10.1016/0143-4179(84)90108-2.PMID: 6087192
Science 220:314-316, 1983), we recently described (Rothman et al, Neuropeptides, in press) the preparation of membranes possessing only lower affinity 3H-DADL binding sites (FIT-treated membranes, type-I delta sites) as well as membranes greatly enriched with higher affini …217Cite
Binding of ligands to the catalytic zinc ion in horse liver alcohol dehydrogenase.
Syvertsen C, McKinley-McKee JS.Arch Biochem Biophys. 1984 Jan;228(1):159-69. doi: 10.1016/0003-9861(84)90057-2.PMID: 6364988
Closely related pyridine compounds gave a regular increase in affinity for the enzyme with increasing basicity, as expected for coordination to a metal ion. The sulfur compounds penicillamine and mercaptoethanol also activated the affinity labeling reaction, but dimercapto …218Cite
Colloidal gold and biotin-avidin conjugates as ultrastructural markers for neural antigens.
van den Pol AN.Q J Exp Physiol. 1984 Jan;69(1):1-33. doi: 10.1113/expphysiol.1984.sp002771.PMID: 6201943 Free article. Review.
Biotin can be easily conjugated to immunoglobulins and, due to its high affinity for avidin (10(15) M-1) which has four biotin binding sites, can be localized with avidin markers, or biotin-avidin-marker complexes. …219Cite
Ziegelhöffer A, Breier A, Dzurba A, Vrbjar N.Gen Physiol Biophys. 1983 Dec;2(6):447-56.PMID: 6088362220Cite
Effects of thiocyanate on cytosol androgen receptor from Shionogi carcinoma 115.
Omukai Y, Nohno T, Ikeda M, Watanabe S, Senoo T, Saito T, Hosokawa K.J Steroid Biochem. 1983 Aug;19(2):1055-9. doi: 10.1016/0022-4731(83)90397-7.PMID: 6887917
When 0.5 M KSCN-treated androgen-cytosol mixture was subjected to gel filtration or (NH4)2SO4 fractionation to remove the salt, a partial recovery (30-40%) in specific binding activity was observed. The binding activity of androgen receptor was unaffected by a treat …221Cite
Activation and changes in the sedimentation properties of rat liver glucocorticoid receptor.
Alexis MN, Djordević-Marković R, Sekeris CE.J Steroid Biochem. 1983 Jun;18(6):655-63. doi: 10.1016/0022-4731(83)90243-1.PMID: 6865409
The state of activation was judged by both DNA-cellulose binding and DEAE-Sephadex A-50 anion exchange chromatography. In isotonic phosphate buffer, unactivated and activated glucocorticoid–receptor complex sedimented as a 5 S and a 4 S peak, respectively. …The state of …222Cite
Spectroscopic and kinetics studies of the inhibition of pig kidney diamine oxidase by anions.
Dooley DM, Golnik KC.J Biol Chem. 1983 Apr 10;258(7):4245-8.PMID: 6403525 Free article.
These bands are assigned as ligand-to-metal charge-transfer transitions, N3-/SCN- leads to Cu(II). One anion/Cu(II) is coordinated in an equitorial position. …Azide and thiocyanate are linear uncompetitive inhibitors with respect to the amine substrate when O2 is …223Cite
Muallem S, Karlish SJ.J Biol Chem. 1983 Jan 10;258(1):169-75.PMID: 6129250 Free article.
Catalytic and regulatory binding sites for ATP on the red cell Ca2+ pump have been investigated using fluorescein isothiocyanate (FITC). …Analysis of the kinetics of inactivation by FITC and protection by ATP reveals the participation of both high and low affinity bin …224Cite
Leyh TS, Sammons RD, Frey PA, Reed GH.J Biol Chem. 1982 Dec 25;257(24):15047-53.PMID: 6294089 Free article.
Superhyperfine coupling between the 17O nucleus and Mn(II) produces a characteristic inhomogeneous broadening of the EPR signals for Mn(II) whenever the 17O is in the first coordination sphere of the metal ion. Previous experiments with ADP regio-specifically labeled with …225Cite
The interaction of vanadate ions with the Ca-ATPase from sarcoplasmic reticulum.
Pick U.J Biol Chem. 1982 Jun 10;257(11):6111-9.PMID: 6210692 Free article.
The enzyme-vanadate complex was identified by the appearance of a time lag in the onset of Ca2+ uptake and by a slowing of the fluorescence quenching response to Ca2+. Ca2+ prevented the binding of vanadate to the enzyme. Pyrophosphate (Kd = 2 mM) and ATP (Kd = 25 microM) …226Cite
McRoberts JA, Erlinger S, Rindler MJ, Saier MH Jr.J Biol Chem. 1982 Mar 10;257(5):2260-6.PMID: 6277890 Free article.
Chloride could be replaced in the buffers by Br-, but not by F-, I-, acetate, nitrate, thiocyanate, sulfate, or gluconate. The effect of Cl- was saturating, and Na+-stimulated 86RB+ uptake as well as K+-stimulated 22Na+ uptake was shown to be dependent on the square of the …227Cite
Lorenson MY, Lee YC, Jacobs LS.J Biol Chem. 1981 Dec 25;256(24):12802-10.PMID: 6118365 Free article.
Inhibitory anions in decreasing potency order were azide greater than thiocyanate greater than fluoride greater than nitrate. Anionic stimulation of the granule membrane Mg2+-ATPase linearized the Lineweaver-Burk plots by shifting the enzyme to its higher Km state. …Anio …228Cite
Strothkamp KG, Lippard SJ.Biochemistry. 1981 Dec 22;20(26):7488-93. doi: 10.1021/bi00529a025.PMID: 7326242
The binding of thiocyanate, azide, and other anions to superoxide dismutase (SOD) containing copper in both the copper and zinc sites of the native enzyme (Cu2Cu2SOD) has been studied. …Parallel behavior was observed for SCN- and N3- binding to a model comp …229Cite
Pick U.Eur J Biochem. 1981 Dec;121(1):187-95. doi: 10.1111/j.1432-1033.1981.tb06448.x.PMID: 6459929 Free article. No abstract available.230Cite
Free radical reactions with proteins and enzymes. The inactivation of bovine carbonic anhydrase B.
Hoe ST, Bisby RH, Cundall RB, Anderson RF.Biochim Biophys Acta. 1981 Nov 13;662(1):55-64. doi: 10.1016/0005-2744(81)90223-0.PMID: 6796125231Cite
Lack of binding of human C3, in its native state, to C3b receptors.
Berger M, Gaither TA, Hammer CH, Frank MM.J Immunol. 1981 Oct;127(4):1329-34.PMID: 7276560 No abstract available.232Cite
Hegyvary C, Jorgensen PL.J Biol Chem. 1981 Jun 25;256(12):6296-303.PMID: 6263913 Free article.
We studied conformational changes of purified renal sodium plus potassium ion-transport adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3) labeled with fluorescein isothiocyanate. Fluorescein covalently binds to the alpha-subunit of the enzyme and inhibits the ATP …233Cite
Soumarmon A, Abastado M, Bonfils S, Lewin MJ.J Biol Chem. 1980 Dec 25;255(24):11682-7.PMID: 7440565 Free article.
The sensitivity of those uptakes to a C1- in equilibrium OH- ionophore and to osmotic swelling suggested they were due to transport rather than to binding. The ATP effect was labile, but dithiothreitol and methanol improved its stability. …234Cite
Reed GH, Leyh TS.Biochemistry. 1980 Nov 25;19(24):5472-80. doi: 10.1021/bi00565a002.PMID: 6257280
In the complex with thiocyanate replacing formate as the stabilizing anion, previous infrared experiments [Reed, G. …The results suggest that for creatine kinase the activating metal ion is bound to all three phosphate groups in the transition state of the reactio …235Cite
Pick U, Karlish SJ.Biochim Biophys Acta. 1980 Nov 20;626(1):255-61. doi: 10.1016/0005-2795(80)90216-0.PMID: 6450619
ATP protected specifically against fluorescein isothiocyanate inhibition, indicating that fluorescein isothiocyanate may react at the nucleotide binding site of the ATPase (probably with a reactive lysine residue). The fluorescein is incorporated almost exclusively into th …236Cite
Asher IM, Phillies GD, Geller RB, Stanley HE.Biochemistry. 1980 Apr 29;19(9):1805-13. doi: 10.1021/bi00550a012.PMID: 7378373237Cite
Mechanisms of inactivation of molybdoenzymes by cyanide.
Coughlan MP, Johnson JL, Rajagopalan KV.J Biol Chem. 1980 Apr 10;255(7):2694-9.PMID: 6244290 Free article.
Following gel filtration to remove excess of reductant and cyanide, the isolated enzymes remain inactive. Thiocyanate, a product of inactivation of the oxidized forms of the xanthine- and aldehyde-oxidizing enzymes by cyanide, is not released during inactivation of the red …238Cite
Conformational analysis by nuclear magnetic resonance: insulin.
Williamson KL, Williams RJ.Biochemistry. 1979 Dec 25;18(26):5966-72. doi: 10.1021/bi00593a032.PMID: 574774
Analysis of difference spectra indicates that this new hexamer (which should be capable of binding six zinc ions) binds 2 equiv of SCN- at two sites which are assumed to be identical and independent (K1 = 10(3), K2 = 2.5 X 10(2) M-1)….239Cite
Johnson RG, Pfister D, Carty SE, Scarpa A.J Biol Chem. 1979 Nov 10;254(21):10963-72.PMID: 40978 Free article.
The chromaffin ghosts were formed by hypo-osmotic lysis of isolated bovine chromaffin granules and extensive dialysis in order to remove intragranular binding components and dissipate the endogenous electrochemical gradients. Upon ATP addition to suspensions of chromaffin …240Cite
Effect of anions on folate binding by isolated brush border membranes from rat kidney.
Selhub J, Gay AC, Rosenberg IH.Biochim Biophys Acta. 1979 Nov 2;557(2):372-84. doi: 10.1016/0005-2736(79)90335-3.PMID: 497188
The characteristics of folate binding by brush border membranes from rat kidney homogenates were investigated. At pH 7.4, binding of [3′,5′,9-3H]-pteroylglutamic acid to membranes containing endogenous folate is inhibited by anions, with chloride being most effectiv …241Cite
Liao TH, McKenzie LJ.J Biol Chem. 1979 Oct 10;254(19):9598-601.PMID: 489554 Free article.242Cite
Neya S, Morishima I.J Biol Chem. 1979 Sep 25;254(18):9107-12.PMID: 479181 Free article.
In order to investigate the effect of the alpha beta subunit contacts on the subunit structure of human adult methemoglobin, the hyperfine shifted proton NMR spectra of several high spin complexes (water, cyanate, thiocyanate, formate, fluoride, and nitrite) and low spin c …243Cite
Functional aspects of the superoxide dismutative action of Cu-penicillamine.
Lengfelder E, Fuchs C, Younes M, Weser U.Biochim Biophys Acta. 1979 Apr 12;567(2):492-502. doi: 10.1016/0005-2744(79)90135-9.PMID: 36162244Cite
Effects of possible beta-cell membrane label, metahexamide-isothiocyanate, on insulin release.
Joost HG.Horm Metab Res. 1979 Feb;11(2):104-6. doi: 10.1055/s-0028-1092689.PMID: 437672
It is suggested that the irreversible stimulation of insulin release by MITC reflects the convalent linkage of the label to the sulfonylurea receptor site, while excess isothiocyanate inhibited insulin release by reacting on less specific binding sites involved in the secr …245Cite
Effect of SCN on potassium contracture in twitch muscle fibers of the frog.
Nagai I, Oota I, Nagai T.Jpn J Physiol. 1979;29(1):61-73. doi: 10.2170/jjphysiol.29.61.PMID: 449123246Cite
Bradbury JH, Howell JR, Johnson RN, Warren B.Eur J Biochem. 1978 Mar 15;84(2):503-11. doi: 10.1111/j.1432-1033.1978.tb12193.x.PMID: 25182 Free article. No abstract available.247Cite
Höbel M, Kreye VA, Pill J.Klin Wochenschr. 1978;56 Suppl 1:147-52. doi: 10.1007/BF01477466.PMID: 732247
In the presence of thiosulfate, the plasma level of thiocyanate rose linearly with the infusion time indicating a rapid detoxification of cyanide released in vivo from SNP, whereas at the end of the infusion of SNP alone no increase in plasma thiocyanate could be me …248Cite
Amino acid transport in brush-border-membrane vesicles isolated from human small intestine.
Lücke H, Haase W, Murer H.Biochem J. 1977 Dec 15;168(3):529-32. doi: 10.1042/bj1680529.PMID: 606251 Free PMC article.
L-Alanine entered the same osmotically reactive space as D-glucose, indicating that transport into the vesicle rather than binding to the membranes was being observed. The uptake rate for L-alanine was higher in the presence of a Na+ gradient than in the presence of a K+ g …249Cite
Levy RS, Villafranca JJ.Biochemistry. 1977 Jul 26;16(15):3293-31. doi: 10.1021/bi00634a003.PMID: 19044
This suggests direct coordination of isocitrate to the Mn(II) but perphaps a different mode of binding for alpha-ketoglutarate. Other studies demonstrated mutually exclusive binding relationships between TPN and TPNH, between Mn-isocitrate and TPNH, and between HCO3 …250Cite
Tropomyosin fragments cleaved at the cysteinyl residue.
Ueno H, Takahashi S, Ooi T.J Biochem. 1977 Jul;82(1):131-8. doi: 10.1093/oxfordjournals.jbchem.a131661.PMID: 561059 Free article. No abstract available.251Cite
Felgner PL, Wilson JE.Arch Biochem Biophys. 1977 Jul;182(1):282-94. doi: 10.1016/0003-9861(77)90309-5.PMID: 18993 No abstract available.252Cite
Cox RP, Hollaway MR.Eur J Biochem. 1977 Apr 15;74(3):575-87. doi: 10.1111/j.1432-1033.1977.tb11427.x.PMID: 856586 Free article.
The ligands were azide, cyanide, fluoride, imidazole, thiocyanate and water. 2. The reduction of Fe(III) myoglobin cyanide led to the transient formation of Fe(II) myoglobin cyanide but no intermediate species were observable during the reductions of the other derivatives. …253Cite
Cholesterol Stimulation of Penetration of Unilamellar Liposomes by Hydrophobic Compounds.
LaBelle EF, Racker E.J Membr Biol. 1977 Mar 8;31(3):301-15. doi: 10.1007/BF01869410.PMID: 321787254Cite
Tannenbaum C, Toggenburger G, Kessler M, Rothstein A, Semenza G.J Supramol Struct. 1977;6(4):519-33. doi: 10.1002/jss.400060406.PMID: 413010
In the presence of an NaSCN gradient phlorizin binds with a high affinity (Kd similar or equal to 4.7 micron) to vesicles derived from brush border membranes of intestinal cells of rabbits. …The apparent affinity for phlorizin is decreased if NaCl is substituted for NaSC …255Cite
A nonelectrogenic H+ pump in plasma membranes of hog stomach.
Sachs G, Chang HH, Rabon E, Schackman R, Lewin M, Saccomani G.J Biol Chem. 1976 Dec 10;251(23):7690-8.PMID: 12175 Free article.
The ionophoric and osmotic sensitivity showed that these ion movements were due to transport rather than binding. The cation selectivity sequences, substrate specificities and action of inhibitors indicated that the transport was a function of K+ATPase activity. …256Cite
Crane RK, Malathi P, Preiser H.Biochem Biophys Res Commun. 1976 Aug 23;71(4):1010-6. doi: 10.1016/0006-291x(76)90755-5.PMID: 971296 No abstract available.257Cite
Sica V, Nola E, Puca GA, Bresciani F.Biochemistry. 1976 May 4;15(9):1915-23. doi: 10.1021/bi00654a019.PMID: 178358
Sodium thiocyanate up to 0.5 M is compatible with a stable estradiol-t-receptor complex during sucrose gradient centrifugation; however, the maximum permissible concentration in 0.1 M during Sephadex G-100 and G-200 chromatography. …These modifications appear to depend o …258Cite
Wikström MK, Saari HT.Mol Cell Biochem. 1976 Mar 26;11(1):17-33. doi: 10.1007/BF01792831.PMID: 5667 Review.
The conformational change is brought about by an electrical field-driven binding of one proton per aa3 to the complex. At pH 7.2 the concentration of the two conformations is equal at a membrane potential of 170 mV corresponding to about 4 kcal/mole. 3. …259Cite
Blair DG, Helinski DR.J Biol Chem. 1975 Nov 25;250(22):8785-9.PMID: 1102543 Free article.260Cite
A kinetic study of mitochondrial calcium transport.
Reed KC, Bygrave FL.Eur J Biochem. 1975 Jul 15;55(3):497-504. doi: 10.1111/j.1432-1033.1975.tb02187.x.PMID: 240699 Free article.
Both permeant acids increase the maximum velocity to an extent dependent on their concentration. The Ca2+-binding site(s) of the carrier contains a group ionizing at pH approximately 7.5 at 0 degrees C, which is functional in the dissociated state. …261Cite
Ceruloplasmin-anicn interaction. A resonance Raman spectroscopic study.
Tosi L, Garnier A, Herve M, Steinbuch M.Biochem Biophys Res Commun. 1975 Jul 8;65(1):100-6. doi: 10.1016/s0006-291x(75)80066-0.PMID: 167763 No abstract available.262Cite
Thauer RK, Fuchs G, Käufer B.Hoppe Seylers Z Physiol Chem. 1975 Jun;356(6):653-62. doi: 10.1515/bchm2.1975.356.s1.653.PMID: 241689
The three activities were found to be equally affected by monovalent anions known to be ligands to transition metals: The enzyme was reversibly inhibited by azide (Ki = 0.004mM), cyanate (Ki = 0.3 mM), thiocyanate (Ki = 1mM), nitrite (Ki = 0.4mM), nitrate (Ki = 6mM) …263Cite
Stimulation of rat liver mitochondrial adenosine triphosphatase by anions.
Ebel RE, Lardy HA.J Biol Chem. 1975 Jan 10;250(1):191-6.PMID: 124729 Free article.
These data are interpreted to indicate the existence of two substrate sites for MgATP and an anion-binding site on the isolated enzyme….264Cite
Yount RG.Adv Enzymol Relat Areas Mol Biol. 1975;43:1-56. doi: 10.1002/9780470122884.ch1.PMID: 890 Review. No abstract available.265Cite
Hepatic sulfite oxidase. Effect of anions on interaction with cytochrome c.
Kessler DL, Rajagopalan KV.Biochim Biophys Acta. 1974 Dec 29;370(2):389-98. doi: 10.1016/0005-2744(74)90100-4.PMID: 4374240 No abstract available.266Cite
Acid-base titration of hemocyanin from Octopus vulgaris Lam.
Salvato B, Ghiretti-Magaldi A, Ghiretti F.Biochemistry. 1974 Nov 5;13(23):4778-83. doi: 10.1021/bi00720a015.PMID: 4429662 No abstract available.267Cite
Cleere WF, O’Regan C, Coughlan MP.Biochem J. 1974 Nov;143(2):465-8. doi: 10.1042/bj1430465.PMID: 4462563 Free PMC article.268Cite
Blok MC, De Gier J, Van Deenen LL.Biochim Biophys Acta. 1974 Oct 29;367(2):210-24. doi: 10.1016/0005-2736(74)90044-3.PMID: 4473216 No abstract available.269Cite
Raman spectroscopic study of the valinomycin–KSCN complex.
Asher IM, Rothschild KJ, Stanley HE.J Mol Biol. 1974 Oct 15;89(1):205-22. doi: 10.1016/0022-2836(74)90171-5.PMID: 4444048 No abstract available.270Cite
Dixon JW, Sarkar B.J Biol Chem. 1974 Sep 25;249(18):5872-7.PMID: 4414013 Free article. No abstract available.271Cite
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Buttlaire DH, Cohn M.J Biol Chem. 1974 Sep 25;249(18):5741-8.PMID: 4369851 Free article. No abstract available.273Cite
Glucocorticoid binding in the chicken liver cytosol.
Cochet C, Chambaz EM.Biochim Biophys Acta. 1974 Aug 7;362(1):37-47. doi: 10.1016/0304-4165(74)90023-3.PMID: 4371348 No abstract available.274Cite
Hirabayashi T, Perry SV.Biochim Biophys Acta. 1974 Jun 7;351(2):273-89. doi: 10.1016/0005-2795(74)90189-5.PMID: 4209892 No abstract available.275Cite
Wallace WJ, Maxwell JC, Caughey WS.Biochem Biophys Res Commun. 1974 Apr 23;57(4):1104-10. doi: 10.1016/0006-291x(74)90810-9.PMID: 4830750 No abstract available.276Cite
Relative affinities of alkali metal ions to the ligands in ionophores.
Balasubramanian D, Misra BC.FEBS Lett. 1974 Apr 15;41(1):78-80. doi: 10.1016/0014-5793(74)80958-0.PMID: 4854091 Free article. No abstract available.277Cite
Menzel J, Steffen C.Immunology. 1974 Feb;26(2):417-23.PMID: 4852359 Free PMC article.278Cite
Dailey HA Jr, Lascelles J.Arch Biochem Biophys. 1974 Feb;160(2):523-9. doi: 10.1016/0003-9861(74)90429-9.PMID: 4364772 No abstract available.279Cite
Trotta PP, Pinkus LM, Haschemeyer RH, Meister A.J Biol Chem. 1974 Jan 25;249(2):492-9.PMID: 4358555 Free article. No abstract available.280Cite
Binding of ATP to Na+, K+-ATPase.
Norby JG, Jensen J.Ann N Y Acad Sci. 1974;242(0):158-67. doi: 10.1111/j.1749-6632.1974.tb19088.x.PMID: 4279585 No abstract available.281Cite
Structural studies of transition state analog complexes of creatine kinase.
Reed GH, McLaughlin AC.Ann N Y Acad Sci. 1973 Dec 31;222:118-29. doi: 10.1111/j.1749-6632.1973.tb15256.x.PMID: 4361852 No abstract available.282Cite
Unemoto T, Tanaka M, Hayashi M.Biochim Biophys Acta. 1973 Dec 19;327(2):490-500. doi: 10.1016/0005-2744(73)90432-4.PMID: 4360431 No abstract available.283Cite
Inhibition of fumarylacetoacetate fumarylhydrolase by monovalent anions.
Braun CV, Schmidt DE Jr.Biochemistry. 1973 Nov 20;12(24):4878-81. doi: 10.1021/bi00748a011.PMID: 4357550 No abstract available.284Cite
Reaction of celite-bound fluorescein isothiocyanate with the 50S E. coli ribosomal subunit.
Hsiung N, Cantor CR.Arch Biochem Biophys. 1973 Jul;157(1):125-32. doi: 10.1016/0003-9861(73)90397-4.PMID: 4352054 No abstract available.285Cite
Anion-binding and the state of copper in caeruloplasmin.
Byers W, Curzon G, Garbett K, Speyer BE, Young SN, Williams RJ.Biochim Biophys Acta. 1973 May 17;310(1):38-50. doi: 10.1016/0005-2795(73)90006-8.PMID: 4351064 No abstract available.286Cite
Kragh-Hansen U, Moller JV.Biochim Biophys Acta. 1973 Feb 21;295(2):438-46.PMID: 4573078 No abstract available.287Cite
Uren JR, Neurath H.Biochemistry. 1972 Nov 21;11(24):4483-92. doi: 10.1021/bi00774a010.PMID: 4675873 No abstract available.288Cite
Krigbaum WR, Kuegler FR, Oelschlaeger H.Biochemistry. 1972 Nov 21;11(24):4548-52. doi: 10.1021/bi00774a018.PMID: 4654142 No abstract available.289Cite
Anion effects on glycine entry into pigeon red blood cells.
Imler JR, Vidaver GA.Biochim Biophys Acta. 1972 Oct 23;288(1):153-65. doi: 10.1016/0005-2736(72)90233-7.PMID: 4640384 No abstract available.290Cite
Amino acid sequence of human erythrocyte carbonic anhydrase B.
Andersson B, Nyman PO, Strid L.Biochem Biophys Res Commun. 1972 Aug 7;48(3):670-7. doi: 10.1016/0006-291x(72)90400-7.PMID: 4625868 No abstract available.291Cite
Studies on succinate dehydrogenase. XX. Amino acid sequence around the flavin site.
Kenney WC, Walker WH, Singer TP.J Biol Chem. 1972 Jul 25;247(14):4510-3.PMID: 4339718 Free article. No abstract available.292Cite
Dixon JW, Sarkar B.Biochem Biophys Res Commun. 1972 Jul 11;48(1):197-200. doi: 10.1016/0006-291x(72)90362-2.PMID: 5064954 No abstract available.293Cite
An anion-sensitive ATPase in lizard gastric mucosa.
de Pont JJ, Hansen T, Bonting SL.Biochim Biophys Acta. 1972 Jul 3;274(1):189-200. doi: 10.1016/0005-2736(72)90293-3.PMID: 4261420 No abstract available.294Cite
Conformational interconversions of the cyclic hexapeptide cyclo(Pro-Gly) 3.
Deber CM, Torchia DA, Wong SC, Blout ER.Proc Natl Acad Sci U S A. 1972 Jul;69(7):1825-9. doi: 10.1073/pnas.69.7.1825.PMID: 4505660 Free PMC article.
In addition, it has been found that this cyclic peptide binds alkali metal cations. While the predominant conformation of this cyclic peptide is 3-fold symmetric in the apolar solvent methylene chloride, an asymmetric structure is preferred in some polar solvents (w …295Cite
Gel chromatographic studies of the dissociation equilibrium in hemerythrin.
Rao AL, Keresztes-Nagy S.Arch Biochem Biophys. 1972 Jun;150(2):493-502. doi: 10.1016/0003-9861(72)90067-7.PMID: 5044038 No abstract available.296Cite
Temperature and competitive anion-binding studies of carbonic anhydrase.
Ward RL, Cull MD.Arch Biochem Biophys. 1972 Jun;150(2):436-9. doi: 10.1016/0003-9861(72)90060-4.PMID: 4625501 No abstract available.297Cite
Denaturation of globular proteins. Interaction of guanidinium salts with three proteins.
Gordon JA.Biochemistry. 1972 May 9;11(10):1862-70. doi: 10.1021/bi00760a021.PMID: 5063539 No abstract available.298Cite
Fee JA, Gaber BP.J Biol Chem. 1972 Jan 10;247(1):60-5.PMID: 4336044 Free article. No abstract available.299Cite
The mechanism of anion permeation in thorium-treated gallbladder.
Machen TE, Diamond JM.J Membr Biol. 1972;8(1):63-96. doi: 10.1007/BF01868095.PMID: 4343307 No abstract available.300Cite
Nonequivalent binding to human methemoglobin. Thermodynamic and kinetic comparison.
Klapper MH, Uchida H.J Biol Chem. 1971 Nov 25;246(22):6849-54.PMID: 5126226 Free article. No abstract available.301Cite
Effect of structural perturbations on the ligand-binding properties of human methemoglobin A.
Uchida H, Heystek J, Klapper MH.J Biol Chem. 1971 Nov 25;246(22):6843-8.PMID: 5126225 Free article. No abstract available.302Cite
[Chemical and immunological aspects of ferrocene marking of antibodies].
Franz H, Wildführ W.Z Immunitatsforsch Exp Klin Immunol. 1971 Nov;142(4):334-41.PMID: 4257659 German. No abstract available.303Cite
The influence of anions on enzymic activity and metal cation binding of leucine aminopeptidase.
Ludewig M, Lasch J, Kettmann U, Frohne M, Hanson H.Enzymologia. 1971 Jul 31;41(1):59-67.PMID: 4329993 No abstract available.304Cite
Chung AE, Franzen JS, Braginski JE.Biochemistry. 1971 Jul 20;10(15):2872-6. doi: 10.1021/bi00791a012.PMID: 4398870 No abstract available.305Cite
Calorimetric studies of quaternary structure and ligand-binding hemerythrin.
Langerman N, Sturtevant JM.Biochemistry. 1971 Jul 20;10(15):2809-15. doi: 10.1021/bi00791a001.PMID: 4329807 No abstract available.306Cite
Succinate dehydrogenase. II. Enzymatic properties.
Hanstein WG, Davis KA, Ghalambor MA, Hatefi Y.Biochemistry. 1971 Jun 22;10(13):2517-24. doi: 10.1021/bi00789a015.PMID: 4326770 No abstract available.307Cite
Knowles AF, Guillory RJ, Racker E.J Biol Chem. 1971 Apr 25;246(8):2672-9.PMID: 4251853 Free article. No abstract available.308Cite
Evidence for nonequivalent binding sitesin human methemoglobin.
Uchida H, Heystk J, Klapper MH.J Biol Chem. 1971 Apr 10;246(7):2031-4.PMID: 5549599 Free article. No abstract available.309Cite
Binding of water and solute to DNA.
Chattoraj DK, Bull HB.Arch Biochem Biophys. 1971 Jan;142(1):363-70. doi: 10.1016/0003-9861(71)90294-3.PMID: 5101856 No abstract available.310Cite
Anion inhibition of firefly luciferase.
Denburg JL, McElroy WD.Arch Biochem Biophys. 1970 Dec;141(2):668-75. doi: 10.1016/0003-9861(70)90187-6.PMID: 5497150 No abstract available.311Cite
Preferential solvation of S-cysteinyl bovine serum albumin in aqueous solutions of lithium salts.
Noelken ME.Biochemistry. 1970 Oct 13;9(21):4122-8. doi: 10.1021/bi00823a014.PMID: 5466418 No abstract available.312Cite
Fluorescent-protein staining of nucleoli in monolayer cell cultures: determinants and significance.
Maisel JC.J Histochem Cytochem. 1970 Oct;18(10):746-55. doi: 10.1177/18.10.746.PMID: 4100511 No abstract available.313Cite
Poisner AM, Hava M.Mol Pharmacol. 1970 Jul;6(4):407-15.PMID: 4246825 No abstract available.314Cite
Jolin T, Morreale de Escobar G, Escobar del Rey F.Endocrinology. 1970 Jul;87(1):99-110. doi: 10.1210/endo-87-1-99.PMID: 4192261 No abstract available.315Cite
Paradoxical temperature dependence of sodium and potassium fluxes in human red cells.
Wieth JO.J Physiol. 1970 May;207(3):563-80. doi: 10.1113/jphysiol.1970.sp009081.PMID: 5499736 Free PMC article.
Passive sodium and potassium fluxes varied in a complex way with temperature, when the isotonic electrolyte media contained 120 mM thiocyanate or salicylate. Between 0 and 18 degrees C the fluxes decreased with increasing temperature. …It is proposed that the anion induc …316Cite
Physicochemical and kinetic properties of mushroom tyrosinase.
Duckworth HW, Coleman JE.J Biol Chem. 1970 Apr 10;245(7):1613-25.PMID: 4985615 Free article. No abstract available.317Cite
Water and solute binding by proteins. 1. Electrolytes.
Bull HB, Breese K.Arch Biochem Biophys. 1970 Apr;137(2):299-305. doi: 10.1016/0003-9861(70)90443-1.PMID: 5439298 No abstract available.318Cite
Shukla PS, Mahadevan S.Arch Biochem Biophys. 1970 Mar;137(1):166-74. doi: 10.1016/0003-9861(70)90423-6.PMID: 5435053 No abstract available.319Cite
Effects of various salts on the binding of indole compounds with bovine albumin.
McMenamy RH, Madeja MI, Watson F.J Biol Chem. 1968 May 25;243(10):2625-30.PMID: 4297267 Free article. No abstract available.320Cite
Marriott J, Perkins DJ.Biochim Biophys Acta. 1968 Apr 9;154(3):501-6. doi: 10.1016/0005-2795(68)90010-x.PMID: 5650417 No abstract available.321Cite
Ion binding by tobacco mosaic virus and its protein.
Shalaby RA, Banerjee K, Lauffer MA.Biochemistry. 1968 Mar;7(3):955-9. doi: 10.1021/bi00843a011.PMID: 5657860 No abstract available.322Cite
Effects of potentiators of muscular contraction on binding of cations by sarcoplasmic reticulum.
Carvalho AP.J Gen Physiol. 1968 Mar;51(3):427-42. doi: 10.1085/jgp.51.3.427.PMID: 4231148 Free PMC article.
Anionic (NO(3) (-), Br(-), I(-), and SCN(-)) and cationic (Zn(++) and Cd(++)) potentiators of the twitch output of skeletal muscle depress the active binding of Ca by sarcoplasmic reticulum isolated from rabbit skeletal muscle. Zinc and Cd exchange for Ca and Mg at the …323Cite
The thiocyanate oxygen equilibrium in “Helix pomatia” haemocyanin.
De Ley M, Lontie R.Arch Int Physiol Biochim. 1968 Feb;76(1):175-6.PMID: 4175040 No abstract available.324Cite
Klapper MH, Klotz IM.Biochemistry. 1968 Jan;7(1):223-31. doi: 10.1021/bi00841a027.PMID: 5758545 No abstract available.325Cite
Pollay M, Stevens A, Davis C Jr.Anal Biochem. 1966 Nov;17(2):192-200. doi: 10.1016/0003-2697(66)90197-7.PMID: 5971417 No abstract available.326Cite
THE BINDING OF HEAVY METAL IONS BY PAPAIN.
HERBLIN WF, RITT PE.Biochim Biophys Acta. 1964 Jun 1;85:489-90. doi: 10.1016/0926-6569(64)90313-x.PMID: 14194864 No abstract available.327Cite
THE ZINC-BINDING GROUPS OF CARBOXYPEPTIDASE A.
COOMBS TL, OMOTE Y, VALLEE BL.Biochemistry. 1964 May;3:653-62. doi: 10.1021/bi00893a010.PMID: 14193635 No abstract available.