Thermolysin is used in the industrial synthesis of aspartame. It catalyzes the formation of the peptide bond between aspartic acid and phenylalanine methyl ester, resulting in aspartame.
Both thermolysin and snake venom metalloproteinases (SVMPs) share a similar mechanism of action, utilizing zinc ions to hydrolyze peptide bonds in proteins
Gomis-Rüth FX, Kress LF, Kellermann J, Mayr I, Lee X, Huber R, Bode W. Refined 2.0 A X-ray crystal structure of the snake venom zinc-endopeptidase adamalysin II. Primary and tertiary structure determination, refinement, molecular structure and comparison with astacin, collagenase and thermolysin. J Mol Biol. 1994 Jun 17;239(4):513-44. doi: 10.1006/jmbi.1994.1392. PMID: 8006965.
Adamalysin II, alias proteinase II, a 24 kDa zinc-endopeptidase isolated from the snake venom of the Eastern diamondback rattlesnake Crotalus adamanteus, is a prototype, of the pr
What Is Metalloproteinase?
A metalloproteinase, or metalloprotease, is any protease enzyme whose catalytic mechanism involves a metal. An example is ADAM12 w